Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells using human group AB (IV) serum
Abstract
This study showed the possibility of using human group AB (IV) serum in the protocol of osteogenic induction of human bone marrow-derived mesenchymal stem cells in vitro. Human bone marrow-derived mesenchymal stem cells were an object of research. The osteogenic differentiation was controlled using the methods of light microscopy, histochemistry (von Kossa and alizarin S red staining) and quantitative RT-PCR. The cultivation of human bone marrow-derived mesenchymal stem cells in the osteoinduction medium with human group AB (IV) serum during 21 days was proved to lead to the differentiation of cells of this type into the osteogenic direction as the evidence of characteristic morphological indications of bone tissue, the formation of ossifications, an increased gene expression of alkaline phosphatase, and osteopontin.
About the Authors
E. V. Kuvyrkov
Белорусский государственный технологический университет; Республиканский научно-практический центр трансфузиологии и медицинских биотехнологий
Belarus
Belarus
I. M. Seviaryn
Республиканский научно-практический центр трансфузиологии и медицинских биотехнологий
Belarus
Belarus
N. A. Belyasova
Белорусский государственный технологический университет
Belarus
Belarus
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Review
For citations:
Kuvyrkov E.V., Seviaryn I.M., Belyasova N.A. Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells using human group AB (IV) serum. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2015;(2):89-91. (In Russ.)
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