Features of the expression of biomolecular markers (CD44, MMP9, TGFβ1) in assessing the effectiveness of treatment of endothelial-epithelial corneal dystrophy
https://doi.org/10.29235/1814-6023-2019-16-4-477-487
Abstract
The purpose was to determine the effectiveness of endothelial-epithelial dystrophy treatment with subconjunctival injection of low molecular weight hyaluronic acid.
Prospective material of 14 corneas with different epithelialepithelial dystrophy forms were examined and treated. To study the morphological features of keratopathy with the background of glaucoma staining of histological preparations with hematoxylin and eosin; histochemical staining (PAS reaction, MSB,Masson,Congored; IHC with CD44, MMP9, TGFβ1 were used.
According to the results obtained, in the group of patients with EED, with the background of the use of an injectable form of hyaluronic acid, the activity of inflammation decreases due to a decrease in the epithelial and stromal expression of CD44 and MMP9. The proapoptotic and profibrotic action of TGFβ1 decreases.
About the Authors
G. R. Semak
Belarusian State Medical University
Belarus
Belarus
Galina R. Semak – Ph. D. (Med.), Assistant Professor.
83, Dzerzhinski Ave., 220116, Minsk
V. A. Zakharova
Belarusian State Medical University
Belarus
Belarus
Viktoria A. Zakharova – Ph. D. (Med.), Assistant Professor.
83, Dzerzhinski Ave., 220116, Minsk
S. K. Kletsky
Belarusian State Medical University
Belarus
Belarus
Semion K. Kletsky – Ph. D. (Med.), Assistant Professor.
83, Dzerzhinski Ave., 220116, Minsk
T. A. Letkovskaya
Belarusian State Medical University
Belarus
Belarus
Tatiana A. Letkovskaya – Ph. D. (Med.), Assistant Professor.
83, Dzerzhinski Ave., 220116, Minsk
I. Yu. Zherko
Belarusian State Medical University
Belarus
Belarus
Iryna Yu. Zherko – Junior researcher.
83, Dzerzhinski Ave., 220116, Minsk
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Review
For citations:
Semak G.R., Zakharova V.A., Kletsky S.K., Letkovskaya T.A., Zherko I.Yu. Features of the expression of biomolecular markers (CD44, MMP9, TGFβ1) in assessing the effectiveness of treatment of endothelial-epithelial corneal dystrophy. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2019;16(4):477-487. (In Russ.) https://doi.org/10.29235/1814-6023-2019-16-4-477-487
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