OPTIMIZATION OF BASOPHIL GATING ALGORITHM ON FLOW CYTOMETRY: MULTICOLOR ANALYSIS
Abstract
Seven strategies of basophil gating were analyzed using multicolor flow cytometry. It was shown that the most effective and reliable gating strategies for BAT were IgEhi and CD123+HLA-DR–. The stability of basophil identification markers depending on the activation status of basophils was assayed. It was established that IgE and CD123 molecules exhibited no significant difference unlike molecules CD203c and CD193. It was shown that the IgEhi and CD123+HLA-DR– regions had the lowest contamination by other cell subsets, which indicates that the both gating strategies may be used successfully for basophil activation test.
About the Authors
I. U. Ramanava
Republican Scientific and Practical Center for Epidemiology and Microbiology
Belarus
Researcher of the Laboratory for Immunology and Cellular Biotechnology
Belarus
Researcher of the Laboratory for Immunology and Cellular Biotechnology
A. Y. Hancharou
Republican Scientific and Practical Center for Epidemiology and Microbiology
Belarus
Belarus
N. I. Dudarava
10th City Clinical Hospital
Russian Federation
Ph. D. (Med.), Head of the Depart-ment of Allergology and Occupational Pathology
Russian Federation
Ph. D. (Med.), Head of the Depart-ment of Allergology and Occupational Pathology
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Review
For citations:
Ramanava I.U., Hancharou A.Y., Dudarava N.I. OPTIMIZATION OF BASOPHIL GATING ALGORITHM ON FLOW CYTOMETRY: MULTICOLOR ANALYSIS. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2016;(4):15-24. (In Russ.)
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