Investigation of mitochondrial oxidation of the small intestine mucosa under the conditions of incorporation of ¹³⁷Cs

In an experiment on white rats, the parameters of mitochondrial oxidation of small intestine tissue during the incorporation of ¹³⁷Cs were studied by polarographic method. The animals of the experimental group received radioactive feed for 14–30 days, the control group was kept on a standard vivarium diet. Experimental groups with the level of accumulation of radionuclides of 600–800, 3 000–3 300 and 10 000 Bq/kg were formed. The assessment of absorbed doses of internal radiation was calculated from the content of ¹³⁷Cs in rat carcasses. The “inverted intestinal sac” method was used to obtain tissue fragments of the small intestine. Dosimetric control was carried out on a gamma-ray spectrometer LP 4900B (Finland). Experimental data were obtained when working with tissue fragments. The studies reflect the high sensitivity of the small intestine tissue to the effects of internal radiation. Significant changes in the studied indicators of energy metabolism have been revealed. A change in the integral index of mitochondrial oxidation (respiration rate on endogenous substrates) was noted, which consistently increased with an increase in the level of caesium incorporation. The greatest uncoupling effect of 2.4-DNF was detected at the incorporation level of 10 000 Bq/kg. It has been shown that the intensity of tissue respiration on both endogenous and exogenous substrates depends on the level of incorporation of ¹³⁷Cs. It has been shown that the intensity of tissue respiration on both endogenous and exogenous substrates depends on the level of incorporation of ¹³⁷Cs.

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