Transplantation of mesenchymal stem cells (MSC) seems to be an effective method for cartilage lesion treatment. However, there is no common approach for selection of a stem cells source, a surgical method of transplantation, a MSC graft differentiation degree. An experiment by the developed method was performed on dogs. Grafts with different degree of differentiation were used. The results revealed that the chondrogenic differentiated MSCs graft allows regenerative recovery of cartilage injury to be achieved.

The aim of this study was to determine the participation of M-cholino- and β-adrenoreactive systems in mechanisms of development of cardioprotective effects of phenomena of remote ischemic pre- and postconditioning in old rats. It was found that the activity of M-cholino- and β-adrenoreactive systems did not play a significant role in mechanisms of development of the antiischemic effect of phenomena of remote ischemic pre- and postconditioning in old rats. Administration of metoprolol before remote ischemic preconditioning enhances the antiarrhythmic effect of phenomena of remote ischemic preconditioning in old rats.

We enrolled 126 patients at the age of 39 to 85 who underwent primary percutaneous coronary intervention for ST-segment elevation acute coronary syndrome. Depending on the development of recurrent coronary events, we divided all patients into 2 groups: with recurrent coronary events (with a high cardiovascular risk, n = 40) and without recurrent coronary events (control group, n = 86). We studied the peculiarities of coronary artery lesions and the influence of АВСВ1 and CYP2C19 genetic polymorphisms.

Plasma levels of prostate-specific antigen, insulin-like growth factor-1, cholesterol, triglyceride were investigated in the patients with prostate cancer and healthy donors. We found a casual relation between metabolic syndrome and prostate cancer.

The formula for assessment of a pulse wave velocity in the common carotid artery of patients with essential arterial hypertension of 1 degree considering a heart rate is proposed. For this formula to be used, minimum data on a patient are required: arterial pressure, pulse rate, and age.

The spread of nosocomial multidrug-resistant strains of P. aeruginosa, A. baumannii and K. pneumoniae brought back the need of colistin use. However, the data concerning pharmacokinetics of colistin is lacking, precluding from optimal dosing of the drug, especially in critically ill patients.The pharmacokinetics of colistin and colistimethate sodium in children with chemotherapy-induced neutropenia was studied. The serum concentration of colistin was determined by high performance liquid chromatography (HPLC) and mass spectrometry. The concentration of colistin after intravenous administration of colistimethate sodium was determined in 21 children (13 patients with sepsis, 8 children in the control group) with chemotherapy-induced neutropenia.The significant variability of pharmacokinetic parameters of colistin in patients with sepsis and in the control group was revealed. HPLC and mass spectrometry can be used for therapeutic drug monitoring and optimizing of the dosing regime.

). Some neuronal stem/progenitor markers (nestin, b3-tubulin, vimentin, p75, GFAP) were observed in the analyzed cells, suggesting that hOM-MSCs may represent a distinct tissue-specific population of stem cells. The presence of co-inhibitory molecules may explain the immunosuppressive effect of MSC on human T-cells.

Every year there is a steady increase in the number of surgeries performed for basal cerebrospinal fluid fistulae plastics. Currently, there are no clearly determined indications for transcranial and endoscopic surgical methods and algorithms for treating such patients in the early postoperative period. We introduced several methods of surgical treatment of nasal liquorrhea that are successfully used in our neurosurgical departments. The article shows the results of different methods of surgical interventions and their comparative analysis.

Co-culture of hematopoietic stem cells from umbilical cord blood with umbilical cord and placental mesenchymal stromal cells resulted in a significant decrease in the expression level of hematopoietic stem cells marker CD34 and an increase in the proportion of unipotent granulocyte and macrophage colony-forming units.

Diagnostic criteria of angiospastic disorders and decreased blood flow in the brain and kidneys in neonates with hypoxic-ischemic and hypoxic-traumatic encephalopathy in 5-14 days are established: basilar artery RI ≥ 0.68 (sensitivity (Se) 83.8 %, specificity (Sp) 92.9 %, likelihood ratio (LR) 11.7), anterior cerebral artery RI ≥ 0.67 (Se 81.0 %, Sp 92.3 %, LR 10.5), middle cerebral artery RI ≥ 0.66 (right Se 77.5 %, Sp 92.3 %, LR 10.1; left Se 77.5 %, Sp 92.3 %, LR 10.1); stem renal artery RI ≥ 0.74 (right Se 70.2 %, Sp 88.9 %, LR 6.3; left Se 72.3 %, Sp 88.9 %, LR 6.5), segmental branches of the renal artery RI ≥ 0.70 (right Se 61.7 %, Sp 77.8 %, LR 2.8; left Se 70.2 %, Sp 83.3 %, LR 4.2), interlobar branches of the renal artery RI ≥ 0.62 (right Se 70.2 %, Sp 77.8 %, LR 3.2; left Se 70.2 %, Sp 72.2 %, LR 2.5).

-weighted MRI revealed the oscillating flow of CSF in the duct of Silvius with its global flow directed either into or out of the ventricles. An asymmetry parameter was introduced to quantify the flow. The results have been considered from both the classical approach and in view of the latest knowledge of cerebral fluid metabolism and the role of aquaporins AQP4 and AQP1 in the development of brain edema.

In this paper we study the processes of apoptosis and necrosis in cultured glioma C6 cells and human fibroblasts FLv in the simulation of microgravity. It is found that under microgravity, the conditions for interaction between the molecules providing contacts of cells with each other change.

The possibilities of atomic force microscopy (AFM) allow one to estimate, in contrast to other methods, a surface topography and a three-dimensional image of an object in its native state, which provides valuable information for a further analysis of the efficacy of vaccine based not only on its composition, but also on its structural features by varying them in the experiment. In the immunorehabilitological laboratory of the Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine” atomic force microscopy was applied in the study of the morphological and functional state of liposomal vaccine Inflexal V and experimental sample No. 1 using AFM NT-206.

(for plasma) are presented. “Photolon” (manufactured by RUP “BelMedPreparaty”) was used as a photosensitizer. The rheological properties of whole blood and plasma of rats (intact, with glioma С6, and with glioma С6 and photosensitizer) at the same temperature and in the range of the shear rates are presented for comparison.

The article considers the main ways of assessment and correction of cardiovascular risks in patients of cardiologic profile, who have a co-morbid pathology of the hepatobiliary system. The article reveals the drawbacks of the existing assessment scales, the difficulties in making a differential diagnosis and in prescribing medication treatment. Criteria are found, which must be taken into account in selecting the therapeutic and diagnostic approach and prevention policy for patients with the co-morbid pathology.

This article reviews the data on the use of bevacisumab both with chemotherapy and with monotherapy in treatment of glioblastoma. The reasonability of using dendrite cells as a major component of specific antitumor vaccines in glioblastoma treatment has been discussed.

The article presents an overview of the recent data on the role of an imbalance between cholinergic and dopaminergic neurotransmission in the development of locomotor and autonomic disorders that accompany Parkinson's disease. Particular attention is paid to a selective role of different subtypes of muscarinic and nicotinic receptors in the allocation of dopamine dopaminergic neurons. Among different subtypes (α7, α6, α4 and β2) of nicotinic cholinergic receptors, of the greatest interest is α7-subunit involved in reducing L-DOPA-induced dyskinesias. Therefore, drugs with their target one or more subtypes of n-cholinergic receptors may be optimal for the modulation induced by chronic administration of L-DOPA dyskinesias.

The studies of brain and renal hemodynamics in neonates with hypoxic brain damage are fragmented and nonintegrated. The data of different authors on the changes in the peripheral resistance of cerebral and renal arteries are contradictory. The relationship of cerebral and renal blood flow in these children is not regulated in detail so far. In addition, the statistical data analysis in most of the analyzed research works was carried out using parametric methods. Experience shows that in medical research, not more than 20 % of quantitative traits satisfy these conditions. To improve the efficiency of the Doppler examination of cerebral and renal blood flow in newborns with hypoxic brain damage, it is necessary to use non-parametric statistical methods of data processing and methods of evidence-based medical practice.

In this article we examine modern data on anatomic and functional disorders in the cardiovascular system during both physiological pregnancy and pregnancy complicated with preeclampsia. We demonstrate the significance of these disorders not only in the development of complications during pregnancy but also cardiovascular diseases in later stages of life. The review considers the nature of gestational and perinatal complications in maternal metabolic syndrome. Basic guidelines for the management of pregnancy in this category of patients are given.