When studying the efficiency of impact of folic acid competitors on intertwined Lewis carcinoma in experimental animals, it is established that 3-(couple-aminobenzene-sulfamido)-5-metilizoksazol and 2,4-diamino-5-(3,4,5-metoksibenzyl)-pyrimidine have a certain therapeutic effect. The mechanism of action consists both of the cytotoxic influence on tumor cells and of a decrease in the level of their proliferation. The noticeable damaging effect on surrounding non-neoplastic cells is not registered.

In order to study whether dopamine agonists have influence on metabolic and hormonal changes in the diet-induced obesity, a set of tests was performed in young male rats (n = 48), divided into groups according to a food caloric value and a pharmacological agent influencing dopamine receptors (DR). A weight gain and a decrease in the heat production intensity of animals take place when a daily energy intake is increased regardless of the DR functional state. Serum leptin concentration and leptin resistance levels decrease in rats with a prolonged administration of DR2 agonist (Bromocriptin®). A regulated growth of the DR2 functional activity in diet-induced obese animals leads to leptin secretion weakening and leptin resistance decrease, which is directed to decrease appetite.

A widespread use of RT-PCR as an effective method of RNA-viruses diagnostics has created a need for stable and relevant controls. Unfortunately, controls based on plasmids and vial viruses have several critical disadvantages. The armored RNA technology shows ways to avoid these limitations. Armored RNA is a complex of MS2 bacteriophage coat protein and RNA produced in Escherichia coli by the induction of an expression plasmid that encodes the coat protein and an RNA control sequence. The RNA sequences are protected from RNase digestion within the pseudoviral complex. These particles can work as a control on every stage of PCR virus detection - lysis, purification, revert transcription and amplification. This article describes the process of development and construction of armored RNA for a subsequent use as control for RT-PCR detection of RNA-viruses.

The quantitative X-ray computed tomography data in 150 patients with cervical osteochondrosis aged 20 to 77 years were analyzed. The dependence of changes in gravitational and antigravitational forces on the elements of the vertebral segment, leading to the deformation of the vertebrae, is established. Possibly, the differenсe in the pathological deformation, which accompanies the development of degenerative-dystrophic changes in the cervical level, is due to the specificity of mineral metabolism and bone density changes.

A preclinical study of biological compatibility and toxicological safety is a necessary condition in the development of new implantable biomaterials. The results of the study prove that titanium implants without coating and with different-type physical and chemical, and solely chemical coating are biologically compatible and toxicologically safe, because they don't cause haemolytic and cytotoxic complications, allergic reactions, system toxic reaction, and local irritation. They don't lead to an inflammatory, alterative or toxic reaction of surrounding tissues, and obey all the necessary requirements for medical technique devices according to these parameters.

The fibrin gel with 4 and 8 mg/ml fibrinogen was proved to maintain and enhance the differentiation of human bone marrow induced mesenchymal stem cells into chondrogenic direction. The efficiency of the chondrogenic differentiation of human bone marrow induced mesenchymal stem cells was higher in the fibrin gel with 8 mg/ml fibrinogen.

The aim of this work was a morphological study of lipofuscin in the liver of rats with experimental cirrhosis. 96 white rats were divided into six experimental groups and the control group. Cirrhosis was induced by long-term intragastric administration of an oil solution of carbon tetrachloride and ethanol by free watering. The results showed that both the main and additional staining techniques allow one to clearly identify the presence of lipofuscin in the liver of experimental rats and its absence in the control animals. The characteristic dynamics of lipofuscin localization and content in the liver during the development of the cirrhotic process were identified. At the beginning of the experiment its main localization was characterized by a connective tissue cells around the vessels, and by the end of the experiment, it also appeared in hepatocytes of individual lobules. It can be assumed that originally pigment accumulates in the cells, the destruction of which leads to its passage into the intercellular substance of newly formed connective tissue. In 19 weeks of the experiment, the total lipofuscin amount increased by a factor of 4.03, as compared to that in 3 weeks. Identified in this study lipofuscin is undoubtedly a result of the pathological process, and it is not a marker of aging and is not a result of metabolic processes in the organ.

and 9.2 ± 1.3 %. The body fat proportion is a reliable (p < 0.05) correlation with the values of the adaptation potential of the circulatory system, the blood pressure, the general hemodynamic parameter, the index of the physical state, the height of the jump. The basal metabolism value in youth athletes is equal to 1804-1892 kcal/day - 1.166-1.185 kcal/kg h, while junior athletes - 2068 ± 60 kcal/day, 1.12 ± 0.01 kcal/kg h. The average basal metabolism value of young athletes is significantly (p < 0.05) correlated with muscle strength hands, the vital lung capacity, the blood pressure, the adaptive capacity of the circulatory system, the general hemodynamic parameter, the body fat proportion. The values of basal metabolism and body fat in athletes during a two-week training camp vary 0.9 and 1.1 %, respectively, which indicates that the average daily food ration is adequate to the physiological requirements of the body, and the physical activity intensity is adequate to the functional state of athletes.

In experiments on 92 sexually mature male rats, the hypothesis about the regulation functions of neurons in the nucleus tractus solitarii (NTS) proline-rich peptide (PRP-1) of hypothalamus is confirmed. Latent periods of responses of neurons NTS after the application of 100 ul of PRP-1 (1000 or 2000 μg/ml) on the surface of the large hemispheres of brain of rats are equal to 1555 ± 205 Ms. The increase in the frequency of discharges of neurons in NTS, on the average, shows by a factor of 2.3 times the impact of the neuropeptide on the transmission of electrical signals in a population of neurons that specialize in providing a responsive answer to the nociceptive incentive. A comparative study of intramuscular and intranasal application demonstrated that PRP-1 is involved in the control of thermal nociceptive reactions. The cardio-vascular responses to the presence in the nasal cavities of neuropeptide PRP-1 in concentrations of 1000, 100, and 10 μg/ml are not observed in contrast to the intramuscular method of introduction. The obtained experimental data point to a perspective study PRP-1 with the aim of developing a new neurotropic drug for the correction of pain syndromes.

. Photodynamic therapy of the liver with Photolon was used to test groups of animals. Electron microscopy of liver tissues was performed. The developed method of photodynamic therapy for liver cirrhosis resulted in a significant reduction in the severity of alternative changes of hepatocytes and lipid infiltration, promoted the activation of biosynthetic processes of the liver. Hyperplasia of the smooth endoplasmic reticulum, lysosomes, and macrophages with signs of high phagocytic activity testified to the activation of protective systems of cells in response to residual effects of the pathogenic factor. Ito cells were mainly in a passive state, indicating a positive influence on the course of collagen formation processes. The study of the results of photodynamic therapy seems to be promising in clinical conditions in patients with liver cirrhosis.

In evaluating the nutritional status of young persons with the use of the correlation analysis it is established that the body mass index has a low diagnostic value. In order to increase the diagnostic value of anthropometry it is recommended to use additionally the indices the "body fat component" and the abdomen circumference-to-height ratio in the medical-flight examination practice.

The aim of the study is to assess the adequacy of the protein nutrition in patients with chronic pancreatitis in the hospital. Dietary intake of patients is characterized by a low energy density and a lack of protein. The values of urinary excretion of total nitrogen and the value of the negative nitrogen balance confirm the presence of the syndrome of gipermetabolizma-hy-percatabolism in patients, more pronounced in the first three days of hospital treatment. These biochemical parameters of blood and urine tests and anthropometric indicators show that the lack of protein providing patients are fully compensated by the body through muscle proteins.

In acute experiments on rats, the study of sleep apnea mechanisms has found that the functional heterogeneity of the structures of the ventral medulla is the basis for the implementation of countervailing effects of hypercapnic stimulus in vivo and for the modeling of the functional failure of these structures (with lidocaine). The blockade of structures of caudal parts of the ventral medulla is accompanied by the activation of the electrical activity of the diaphragm due to the "disinhi-bition" in these conditions of neurons of rostral parts of the ventral medulla. Inhibition of the functions of structures of rostral parts of the ventral medulla is accompanied by an irreversible violation of the electrical activity of the diaphragm and respiratory arrest.

The method of application of mesenchymal cells in the peripheral parts of the cranial nerves (Gasser's node area in Meckel's space) in effort to analyze the tracing dynamics of these cells in the nucleus of the trigeminal nerve in the brain stem was developed in experiments on rats. It has been established that mesenchymal cells are detected not only in the nuclei of the trigeminal nerve in the brain stem, but also in the destroyed cerebellum areas after the preliminary damage of cerebellum areas.

Alpha2-adrenergic agonist clonidine and its antihypertensive effects are widely used in experimental studies of neu-rotransmitter systems. The aim of this work was to study the characteristics of the electrical activity in the caudal part of the brain stem after the application of clonidine on the nasal mucosa of the rat. Multicellular electrical activity of neurons was recorded in the nucleus of the solitary tract in animals anesthetized by nembutal-urethane. After the application of 50 mkl of 0.1 % clonidine on the nasal mucosa after a 1 min increase in the activity of neurons of the nucleus of the solitary tract was marked from 55 to 112 pulses/s. The phenomenon of anesthesia recovery was discovered after the application of clonidine and hypothetically due to an increase in the previous electrical activity in the brain stem.

This study showed the possibility of using human group AB (IV) serum in the protocol of osteogenic induction of human bone marrow-derived mesenchymal stem cells in vitro. Human bone marrow-derived mesenchymal stem cells were an object of research. The osteogenic differentiation was controlled using the methods of light microscopy, histochemistry (von Kossa and alizarin S red staining) and quantitative RT-PCR. The cultivation of human bone marrow-derived mesenchymal stem cells in the osteoinduction medium with human group AB (IV) serum during 21 days was proved to lead to the differentiation of cells of this type into the osteogenic direction as the evidence of characteristic morphological indications of bone tissue, the formation of ossifications, an increased gene expression of alkaline phosphatase, and osteopontin.

The analysis of the dynamics of clinical and nerve conduction parameters in patients with dysmetabolic (diabetic and alcoholic) polyneuropathy after the course of polyregional magnetic stimulation was performed. It was proved that the predictors of efficiency of this technique are the severity of polyneuropathy rated on TSS and NDSm scales, as well as the disease duration and the HBa1c level in patients with diabetes mellitus type 2.

M decrease the level of the phospholipase activity of staphylococcus by 43.8 %. A complex effect of salicylic acid and copper sulfate on the functional activity of Pseudomonas hemolysin is expressed in the hemolysis suppression by a factor of 2.4 (p < 0.05).

The effect of human lactoferrin derived from the milk of transgenic goats on the testosterone levels in homogenate tissues of the hypothalamus, testes, and blood serum of rats is studied. The effective concentration of the protein with its system use for the stimulation of testosterone biosynthesis is determined.

The discussion material presented in the review of suppressive therapy used in patients with operated high differentiated thyroid cancer justifies the need in suppressive therapy in effort to suppress the proliferation of residual thyroid cells and to reduce a risk of a re-growth of tumors. Along with this, the data on the side effects of subclinic hyperthyroidism developing due to the use of supraphysiological thyroxine doses are presented. Having considered the literature data, normative documents and own experience, it is expedient to find a balanced approach allowing both for the risks of a re-growth of tumors and for the risks of side effects of levothyroxine suppressive therapy.

The data on the bacteriophage role in the human body have been summarized. Key aspects of interaction of the human immune system and bacteriophage particles - components of prophylactic-therapeutic biopreparations have been considered.

A complex of natural and anthropogenic factors, such as a level of urbanization, a quality of air and water, a high level of noise, vibration and electromagnetic emanation, socioeconomic conditions (living conditions, caloric capacity and food quality, information stress) influences the children's organism in big cities. The goal of this article is the assay of principal progress of modern science in the ecological aspect and the adaptative capacity of children to environmental factors. Many factors (biological and sociological) in big cities than in the rural area influence children. This consists in the diversity of the process of adaptation and physical development of children. A main focus is paid to the factors of stress, exceptionally the mechanisms of adaptation of intensive pressure on anthropogenic factors.