Effectiveness of wound coatings with chitosan nanofibers in combination with necrectomy in frostbite treatment in experiment

The problem of treatment of the patients with deep frostbite is still relevant due to its great social and economic significance, which necessitates further research. In general injuries structure, the proportion of cold injury can reach 10 %. The variety of the pathophysiological cryotrauma mechanisms explains the treatment complexity, which leads to frequent complications and significant disability of patients, among them the persons of working age are predominate. However, the generally accepted practice of managing patients with deep frostbite has not yet been developed, for example, the effectiveness of various drugs for local treatment has not been substantiated, the timing and scope of surgical benefits have not been determined. The aim of the study was to examine the effectiveness of wound coatings with chitosan nanofibers in combination with necrectomy on the 5th day during deep contact frostbite treatment. On the 5th day after modeling the standard deep experimental frostbites in 45 laboratory rats, the non-viable tissues in the cryopreservation zone were removed. For treatment of wound defects, the Mekol ointment, sterile gauze napkins, and wound coatings with chitosan nanofibers “Hitomed-wound healing” were used. Reparative processes in the frostbite area were assessed histologically, morphometrically, and statistically. The effectiveness of wound coatings with chitosan nanofibers in combination with necrectomy of the cryoinjured zone on the 5th day in the deep frostbite treatment was established.

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