Expression of the Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake
https://doi.org/10.29235/1814-6023-2022-19-1-62-69
Abstract
The changes in the expression of the lipogenic Fasn and Sirt4 genes and the Fasn and Sirt4 proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake were studied.
In addition to the standard vivarium diet, the rats received the animal fats (45 % of daily caloric value) for 8 weeks. A relative gene expression was determined by real-time PCR, protein content in the visceral adipose tissue - by the ELISA method.
It was found that the excess animal fat intake leads to a decreased expression of lipogenic Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats, which indicates the formation of the adipose tissue dysfunction, which may result in the impared lipid and carbohydrate metabolism, the insulin resistance development.
About the Authors
O. Y. Poluliakh
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Olga Y. Poluliakh – Researcher, Institute of Physiology of the National Academy of Sciences of Belarus.
28, Akademicheskaya Str., 220072, Minsk.
E. I. Kalinovskaya
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Elena I. Kalinovskaya - Ph. D. (Med.), Leading Researcher, Institute of Physiology of the National Academy of Sciences of Belarus.
28, Akademicheskaya Str., 220072, Minsk.
A. A. Basalai
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Anastasia A. Basalai – Researcher, Institute of Physiology of the National Academy of Sciences of Belarus.
28, Akademicheskaya Str., 220072, Minsk.
A. S. Mihalevich
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Anastasia S. Mihalevich - Junior Researcher, Institute of Physiology of the National Academy of Sciences of Belarus.
28, Akademicheskaya Str., 220072, Minsk.
References
1. Khamnueva L. Yu., Andreeva L. S., Kosikova I. N. Obesity. Classification, etiology, pathogenesis, clinical picture, diagnosis, treatment: study guide. Irkutsk, Irkutsk State Medical University Publ., 2007. 33 p. (in Russian).
2. Poltorak V. V., Krasova N. S., Gorshunskaya M. Yu. Sirtuins as a promising targets for the prevention and therapy of diabetes mellitus. Problemi endokrynnoi patologii [Problems of endocrine pathology], 2014, no. 3, pp. 97-104 (in Russian).
3. Min Zh., Gao J., Yu Y. The roles of mitochondrial SIRT4 in cellular metabolism. Frontiers in Endocrinology, 2019, vol. 9, art. 783. https://doi.org/10.3389/fendo.2018.00783
4. Loftus T. M., Jaworsky D. E., Frehywot G. L., Townsend C. A., Ronnett G. V., Lane M. D., Kuhajda F. P. Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors. Science, 2000, vol. 288, no. 5475, pp. 2379-2381. https://doi.org/10.1126/science.288.5475.2379
5. Kumar M. V., Shimokawa T., Nagy T. R., Lane M. D. Differential effects of a centrally acting fatty acid synthase inhibitor in lean and obese mice. Proceedings of the National Academy of Sciences, 2002, vol. 99, no. 4, pp. 1921-1925. https://doi.org/10.1073/pnas.042683699
6. Mayas M. D., Ortega F. J., Madas-Gonzalez M., Bernal R., Gomez-Huelgas R., Fernandez-Real J. M., Tinahones F. J. Inverse relation between FASN expression in human adipose tissue and the insulin resistance level. Nutrition and Metabolism, 2010, vol. 7, no. 1, pp. 1-7. https://doi.org/10.1186/1743-7075-7-3
7. Schmid B., Rippmann J. F., Tadayyon M., Hamilton B. S. Inhibition of fatty acid synthase prevents preadipocyte differentiation. Biochemical and Biophysical Research Communications, 2005, vol. 328, no. 4, pp. 1073-1082. https://doi.org/10.1016/j.bbrc.2005.01.067
8. Hariri N., Thibault L. High-fat diet-induced obesity in animal models. Nutrition Research Reviews, 2010, vol. 23, no. 2, pp. 270-299. https://doi.org/10.1017/S0954422410000168
9. Kravchuk E. N., Galagudza M. M. Experimental models of metabolic syndrome. Arterial'naya gipertenziya [Arterial hypertension], 2014, vol. 20, no. 5, pp. 377-383 (in Russian).
10. Diaz-Villasenor A. A high fat diet reduces the expression of lipogenic, lipolytic and oxidative genes in white adipose tissue. The effect of the concentration and type of fatty acid is dependent of the dietary protein. FASEB Journal, 2010, vol. 24, p. 938.11. https://doi.org/10.1096/fasebj.24.1_supplement.938.11
11. Eissing L., Scherer T., Todter K., Knippschild U., Greve J. W., Buurman W. A. [et al.] De novo lipogenesis in human fat and liver is linked to ChREBP-band metabolic health. Nature Communications, 2013, vol. 4, no. 1, pp. 1-11. https://doi.org/10.1038/ncomms2537
12. Berndt J., Kovacs P., Ruschke K., Kloting N., Fasshauer M., Schon M. R., Korner A., Stumvoll M., Bluher M. Fatty acid synthase gene expression in human adipose tissue: association with obesity and type 2 diabetes. Diabetologia, 2007, vol. 50, no. 7, pp. 1472-1480. https://doi.org/10.1007/s00125-007-0689-x
13. Moreno-Indias I., Tinahones F. J. Impaired adipose tissue expandability and lipogenic capacities as ones of the main causes of metabolic disorders. Journal of Diabetes Research, 2015, vol. 2015, art. 970375. https://doi.org/10.1155/2015/970375
14. Tkachuk V. A., Vorotnikov A. V. Molecular mechanisms of development of insulin resistance. Sakharnyi diabet [Diabetes mellitus], 2014, no. 2, pp. 29-40 (in Russian).
15. Zaganjor E., Vyas S., Haigis M. C. SIRT4 is a regulator of insulin secretion. Cell Chemical Biology, 2017, vol. 24, no. 6, pp. 656-658. https://doi.org/10.1016/j.chembiol.2017.06.002
16. Lopaschuk G. D. Fatty acid oxidation and its relation with insulin resistance and associated disorders. Annals of Nutrition and Metabolism, 2016, vol. 68, suppl. 3, pp. 15-20. https://doi.org/10.1159/000448357
17. Fernandez-Real J. M., Menendez J. A., Moreno-Navarrete J. M., Bluher M., Vazquez-Martin A., Vazquez M., Ortega J. F., Dieguez C., Fruhbeck G., Ricart W., Vidal-Puig A. Extracellular fatty acid synthase: a possible surrogate biomarker of insulin resistance. Diabetes, 2010, vol. 59, no. 6, pp. 1506-1511. https://doi.org/10.2337/db09-1756
18. Abdulkadirova F. R., Ametov A. S., Doskina E. V., Pokrovskaya R. A. The role of lipotoxicity in the pathogenesis of type 2 diabetes mellitus and obesity. Ozhireniye i metabolizm [Obesity and metabolism], 2014, vol. 11, no. 2, pp. 8-12 (in Russian).
Review
For citations:
Poluliakh O.Y., Kalinovskaya E.I., Basalai A.A., Mihalevich A.S. Expression of the Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2022;19(1):62-69. (In Russ.) https://doi.org/10.29235/1814-6023-2022-19-1-62-69
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