Expression of the  Fasn  and  Sirt4  genes and the proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake

O. Y. Poluliakh; E. I. Kalinovskaya; A. A. Basalai; A. S. Mihalevich

doi:10.29235/1814-6023-2022-19-1-62-69

Expression of the Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake

O. Y. Poluliakh, E. I. Kalinovskaya, A. A. Basalai, A. S. Mihalevich

https://doi.org/10.29235/1814-6023-2022-19-1-62-69

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Abstract

The changes in the expression of the lipogenic Fasn and Sirt4 genes and the Fasn and Sirt4 proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake were studied.

In addition to the standard vivarium diet, the rats received the animal fats (45 % of daily caloric value) for 8 weeks. A relative gene expression was determined by real-time PCR, protein content in the visceral adipose tissue - by the ELISA method.

It was found that the excess animal fat intake leads to a decreased expression of lipogenic Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats, which indicates the formation of the adipose tissue dysfunction, which may result in the impared lipid and carbohydrate metabolism, the insulin resistance development.

Keywords

obesity, gene expression, adipose tissue dysfunction, insulin resistance, metabolic disorders

References

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For citations:

Poluliakh O.Y., Kalinovskaya E.I., Basalai A.A., Mihalevich A.S. Expression of the Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2022;19(1):62-69. (In Russ.) https://doi.org/10.29235/1814-6023-2022-19-1-62-69

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ISSN 1814-6023 (Print)
ISSN 2524-2350 (Online)

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Proceedings of the National Academy of Sciences of Belarus, Medical series

Expression of the Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake

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