Experimental model for assessing the readiness of the wound surface to accept skin grafts
https://doi.org/10.29235/1814-6023-2021-18-3-340-350
Abstract
The aim of the study, performed on 65 Wistar rats, was to develop a new model and, as an example, use it to identify the extent to which quercetin and the quercetin-2-hydroxypropyl-β-cyclodextrin nanocomplex, applied to the wound, affect the engraftment of skin autografts. Grafts were obtained from the ear shells of animals, and graft’s survival rate was assessed by the degree of inhibition of wound contraction. It is shown that the latter depends on the area of grafts and the time after which they were located on the wound surface after its creation. It was shown that quercetin worsens, and quercetin-2- hydroxypropyl-β-cyclodextrin does not impair the ability of the wound surface to accept the graft.
Keywords
quercetin, nanocomplex quercetin-2-hydroxypropyl-β-cyclodextrin,
full-layer skin wound,
auricle,
autotransplantation,
wound contraction
Supporting agencies: The work was carried out with the financial support of the Belarusian Republican Foundation for Basic Research (BRFFD, contract No. М19М-145, No. state registration 20.191.840).
About the Authors
A. A. Bakunovich
Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus
Belarus
Belarus
Aliaksei A. Bakunovich – Junior Researcher
50, BLK, 230030, Grodno
Ju. V. Yarashenka
Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus
Belarus
Belarus
Juliya V. Yarashenka – Junior Researcher
50, BLK, 230030, Grodno
E. F. Raduta
Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus
Belarus
Belarus
Elena F. Raduta – Senior Researcher
50, BLK, 230030, Grodno
E. V. Kononov
Grodno State Medical University
Belarus
Belarus
Evgeny V. Kononov – Assistant
80, Gorky Str., 230009, Grodno
V. U. Buko
Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus
Belarus
Belarus
Vyacheslav U. Buko – D. Sc. (Biol.), Professor, Head of the Department
50, BLK, 230030, Grodno
A. A. Astrowski
Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus
Belarus
Belarus
Alexander A. Astrowski – D. Sc. (Med.), Professor, Leading Researcher
50, BLK, 230030, Grodno
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Review
For citations:
Bakunovich A.A., Yarashenka J.V., Raduta E.F., Kononov E.V., Buko V.U., Astrowski A.A. Experimental model for assessing the readiness of the wound surface to accept skin grafts. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2021;18(3):340-350. (In Bel.) https://doi.org/10.29235/1814-6023-2021-18-3-340-350
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