Osteogenic and chondrogenic differentiation potential of mesenchymal stem cells obtained from the bone marrow and placenta
https://doi.org/10.29235/1814-6023-2021-18-1-36-45
Abstract
Mesenchymal stem cells (MSC) represent a perspective resource for cell biotechnology. However the question of chondrogenic and osteogenic capacity of MSC of different origin remains under study.
The aim of this study was to analyze the osteo-chondrogenic differentiation potential of MSC obtained from the bone marrow and placenta. The results of our studies have indicated that bone marrow-derived and placenta-derived MSC showed a chondrogenic potential in vitro after a chondrogenic induction with specific differentiation media. But for bone marrowderived MSC, the chondrogenic program was realized by expression of collagens (Coll2, Coll10), while in placenta-derived MSC cultures we found a progressive increase in COMP and Ver expression, so bone marrow-derived MSC is more preferable for use in cartilage tissue engineering. Regarding the results on alkaline phosphatase and alizarin red staining, bone marrowderived MSC showed a more significant osteogenic potential compared to placenta-derived MSC. Bone marrow-derived MSC in the composition of fibrin gel after osteogenic induction on the 14th day exhibited the activity of alkaline phosphatase, calcium depositions inside the cells and extracellular matrix, the increase in Sp7 and DMP expression.
About the Authors
H. A. Zhernasechanka
Republican Research Center for Pediatric Oncology, Hematology and Immunology
Belarus
Belarus
Hanna A. Zhernasechanka – Researcher
43, Frunzenskaya Str., 223053, v. Borovliany, Minsk Region, Republic of Belarus
Ya. I. Isaikina
Republican Research Center for Pediatric Oncology, Hematology and Immunology
Belarus
Belarus
Yanina I. Isaikina ‒ Ph. D. (Biol.), Head of the Laboratory
43, Frunzenskaya Str., 223053, v. Borovliany, Minsk Region, Republic of Belarus
T. V. Filipovich
Republican Research Center for Pediatric Oncology, Hematology and Immunology
Belarus
Belarus
Tatiana V. Filipovich ‒ Junior Researcher
43, Frunzenskaya Str., 223053, v. Borovliany, Minsk Region, Republic of Belarus
E. G. Liakh
Republican Research Center for Pediatric Oncology, Hematology and Immunology
Belarus
Belarus
Elena G. Liakh ‒ Researcher
43, Frunzenskaya Str., 223053, v. Borovliany, Minsk Region, Republic of Belarus
References
1. Rakic R., Bourdon B., Demoor M., Maddens St., Saulnier N., Galéra Ph. Differences in the intrinsic chondrogenic potential of equine umbilical cord matrix and cord blood mesenchymal stromal/stem cells for cartilage regeneration. Scientific Reports, 2018, vol. 88, art. 13799. https://doi.org/10.1038/s41598-018-28164-9
2. Mushahary D., Spittler A., Kasper C., Weber V., Charwat V. Isolation, cultivation, and characterization of human mesenchymal stem cells. Cytometry. Part A, 2018, vol. 93, no. 1, pp. 19–31. https://doi.org/10.1002/cyto.a.23242
3. Dominici M., le Blanc K., Mueller I., Slaper-Cortenbach I., Marini F. C., Krause D. S., Deans R. J., Keating A., Prockop D. J., Horwitz E. M. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 2006, vol. 8, no. 4, pp. 315–317. https://doi.org/10.1080/14653240600855905
4. Heo J. S., Choi Y., Kim H. S., Kim H. O. Comparison of molecular profiles of human mesenchymal stem cells derived from bone marrow, umbilical cord blood, placenta and adipose tissue. International Journal of Molecular Medicine, 2016, vol. 37, no. 1, pp. 115–125. https://doi.org/10.3892/ijmm.2015.2413
5. Bernardo M. E., Emons J. A. M., Karperien M., Nauta A. J., Willemze R., Roelofs H. [et al.]. Human mesenchymal stem cells derived from bone marrow display a better chondrogenic differentiation compared with other sources. Journal Connective Tissue Research, 2007, vol. 48, no. 3, pp. 132–140. https://doi.org/10.1080/03008200701228464
6. Hsu Sh., Huang T., Cheng Sh., Weng S. Y., Tsai Ch. L., Tseng Ch. Sh., Chen D. Ch., Liu T. Y., Fu K. Y., Yen B. L. Chondrogenesis from human placenta-derived mesenchymal stem cells in three-dimensional scaffolds for cartilage tissue engineering. Tissue Engineering. Part A, 2011, vol. 17, no. 11–12, pp. 1549–1560. https://doi.org/10.1089/ten.tea.2010.0419
7. Beeravolu N., Khan I., McKee C., Dinda S., Thibodeau B., Wilson G., Perez-Cruet M., Bahado-Singh R., Chaudhry G. R. Isolation and comparative analysis of potential stem/progenitor cells from different regions of human umbilical cord. Stem Cell Research, 2016, vol. 16, no. 3, pp. 696–711. https://doi.org/10.1016/j.scr.2016.04.010
8. Kock L., van Donkelaar C. C., Ito K. Tissue engineering of functional articular cartilage: the current status. Cell and Tissue Research, 2012, vol. 347, no. 3, pp. 613–627. https://doi.org/10.1007/s00441-011-1243-1
9. Muraglia A., Cancedda R., Quarto R. Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. Journal of Cell Science, 2000, vol. 113, pt. 7, pp. 1161–1166.
10. Russell K. C., Phinney D. G., Lacey M. R., Barrilleaux B. L., Meyertholen K. E., O’Connor K. C. In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment. Stem Cells, 2010, vol. 28, no. 4, pp. 788–798. https://doi.org/10.1002/stem.312
11. Kim Y. H., Yoon D. S., Kim H. O., Lee J. W. Characterization of different subpopulations from bone marrow-derived mesenchymal stromal cells by alkaline phosphatase expression. Stem Cells and Development, 2012, vol. 21, no. 16, pp. 2958–2968. https://doi.org/10.1089/scd.2011.0349
12. Martinez E. F., Herdy da Silva L. A., Furuse Cr., Soares de Araújo N., Cavalcanti de Araújo V. Dentin matrix protein 1 (DMP1) expression in developing human teeth. Brazilian Dental Journal, 2009, vol. 20, no. 5, pp. 365–369. http://dx.doi.org/10.1590/S0103-64402009000500002]
13. Kamilov F. Kh., Farshatova E. R., Enikeev D. A. Cell-molecular mechanisms of bone tissue remodeling and its regulation. Fundamental’nye issledovaniya [Fundamental research], 2014, no. 7-4, pp. 836–842 (in Russian).
14. Hutchings G., Moncrieff L., Dompe C., Janowicz K., Sibiak R., Bryja A. [et al.]. Bone regeneration, reconstruction and use of osteogenic cells from basic knowledge, animal models to clinical trials. Journal of Clinical Medicine, 2020, vol. 9, no. 1, pii E139. https://doi.org/10.3390/jcm9010139
15. Lee M. H., Javed A., Kim H. J., Shin H. I., Gutierrez S., Choi J. Y. [et al.]. Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor beta1 in C2C12 myogenic cells coincides with suppression of the myogenic phenotype but is not suffcient for osteoblast differentiation. Journal of Cellular Biochemistry, 1999, vol. 73, no. 1, pp. 114–125. https://doi.org/10.1002/(sici)1097-4644(19990401)73:1<114::aid-jcb13>3.0.co;2-m
Review
For citations:
Zhernasechanka H.A., Isaikina Ya.I., Filipovich T.V., Liakh E.G. Osteogenic and chondrogenic differentiation potential of mesenchymal stem cells obtained from the bone marrow and placenta. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2021;18(1):36-45. (In Russ.) https://doi.org/10.29235/1814-6023-2021-18-1-36-45
Views: 800
Email this article 