Changes in the concentration of sulfur-containing amino acids in the brain after methionine load in the experimentChanges in the concentration of sulfur-containing amino acids in the brain after methionine load in the experiment
https://doi.org/10.29235/1814-6023-2020-17-4-461-469
Abstract
The effect of methionine overload on the state of the pool of sulfur-containing amino acids and their metabolites was studied in the various brain structures determined by reverse phase high performance liquid chromatography (HPLC). In all regions of the brain studied, methionine led to a unidirectional imbalance of sulfur-containing compounds: there was an increase in the concentrations of methionine, cystathionine and hypotaurine. The most pronounced increase in methionine and hypotaurine levels was observed in the striatum, cystathionine in the hemispheres. A significant increase in taurine concentration was observed only in the hypothalamus and striatum. In other parts of the brain a tendency to increase its level was shown. In all brain regions studied except the striatum, serine levels were decreased. In the cerebellum, in comparison with other regions, an increase in the level of cysteic acid and a decrease in the level of cysteinesulfinic acid were observed, which indicates that taurine synthesis is occurred mainly through the cysteine sulfinate oxidation.
About the Authors
Ya. I. Novogrodskaya
Grodno State Medical University
Belarus
Belarus
Yana I. Novogrodskaya ‒ Postgraduate student
80, Gorky Str., 230015, Grodno
Ye. M. Doroshenko
Grodno State Medical University
Belarus
Belarus
Yevgeny M. Doroshenko ‒ Ph. D. (Biol.), Assistant Professor, Leading Researche
80, Gorky Str., 230015, Grodno
M. N. Kurbat
Grodno State Medical University
Belarus
Belarus
Mikhail N. Kurbat ‒ Ph. D. (Med.), Assistant Professor, Head of the Laboratory
80, Gorky Str., 230015, Grodno
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For citations:
Novogrodskaya Ya.I., Doroshenko Ye.M., Kurbat M.N. Changes in the concentration of sulfur-containing amino acids in the brain after methionine load in the experimentChanges in the concentration of sulfur-containing amino acids in the brain after methionine load in the experiment. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2020;17(4):461-469. (In Russ.) https://doi.org/10.29235/1814-6023-2020-17-4-461-469
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