Experimental justification of the method of mesenchymal stem cell autotransplantation for regeneration of the femoral head bone tissue
https://doi.org/10.29235/1814-6023-2020-17-1-7-19
Abstract
The results of experimental justification of a new method for restoring the femoral head bone tissue with the use of mesenchymal stem cells (MSCs) are presented. Surgical interventions were performed on 5 dogs, including the bone marrow exfusion, the obtaining of MSCs and their subsequent osteogenic differentiation, the selection of a carrier for MSCs, the creation of femoral head defects, and the implantation of a cellular product into a bone defect. Experimental animals were observed and cared after operations, and removed from the experiment, as well as the biological material was taken. The histological results of the femoral head micropreparations of dogs confirm the formation of the mature bone tissue of compact structure with osteoblasts at the site of MSCs injection. In the control group of the experimental samples (without MSCs), the The obtained experimental material will allow us to develop a method for autologous transplantation of MSCs for regenerative restoration of bone tissue damage in the adult’s femoral head osteonecrosis, which will find use in the practical healthcare system.
About the Authors
A. E. Murzich
Republican Scientific and Practical Centre of Traumatology and Orthopedics
Belarus
Belarus
Alyaksandr E. Murzich – Ph. D. (Med.), Head of the Laboratory
60, Bld. 4, Kizhevatov Str., 220024, Minsk
L. A. Pashkevich
Republican Scientific and Practical Centre of Traumatology and Orthopedics
Belarus
Belarus
Lyudmila A. Pashkevich – D. Sc. (Med.), Head of the Laboratory
60, Bld. 4, Kizhevatov Str., 220024, Minsk
H. A. Zhernasechanka
Republican Research Center for Pediatric Oncology, Hematology and Immunology
Belarus
Belarus
Hanna A. Zhernasechanka – Researcher
43, Frunzenskaya Str., v. Borovliany, 223053, Minsk Region
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Review
For citations:
Murzich A.E., Pashkevich L.A., Zhernasechanka H.A. Experimental justification of the method of mesenchymal stem cell autotransplantation for regeneration of the femoral head bone tissue. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2020;17(1):7-19. (In Russ.) https://doi.org/10.29235/1814-6023-2020-17-1-7-19
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