Pool of free amino acids in the blood plasma of rats undergoing subtotal cerebral ischemia after L-name administration
https://doi.org/10.29235/1814-6023-2019-16-2-185-191
Abstract
A stroke is one of the leading causes of morbidity, disability and mortality in many countries. Mechanisms of development of ischemic stroke are complex and have not been fully established.
The aim of this study was to estimate the changes in the pool of free amino acids and their derivatives in the plasma of rats undergoing subtotal cerebral ischemia (SCI) and treated with L-NAME.
Experiment was made on 18 rats: 12 animals were undergoing bilateral flament occlusion of arteries carotid, 6 of them were treated with L-NAME. The analyses of free amino acids levels in the blood plasma extracts were carried out by reversedphase HPLC. Concentrations of several amino acids were elevated after 1 hour of ischemia, including aspartate, asparagine, glutamine, glycin, alanine, taurine, phenylalanine, gistidine, 3-methilgistidine, treonine, citrulline, ornitine, as well as branched-chain amino acids.
Administration of L-NAME partially prevented the imbalance of the amino acids pool caused by SCI. Preventive injection of L-NAME alleviated the imbalance in the pool of free amino acids of blood plasma caused by SCI.
About the Authors
Y. E. Razvodovsky
Grodno State Medical University
Belarus
Belarus
Yury E. Razvodovsky – Researcher
80, Gorky Str., 230009, Grodno
V. Y. Smirnov
Grodno State Medical University
Belarus
Belarus
Vitaly Yu. Smirnov – Ph. D. (Biol.), Assistant Professor, Senior researcher
80, Gorky Str., 230009, Grodno
N. Ye. Maksimovich
Grodno State Medical University
Belarus
Belarus
Natalia Ye. Maksimovich – D. Sc. (Med.), Professor, Head of the Department
80, Gorky Str., 230009, Grodno
I. N. Semenenya
Institute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus
Belarus
Belarus
Igor N. Semenenya – D. Sc. (Med.), Professor, Director
50, BLK, 230030, Grodno
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Review
For citations:
Razvodovsky Y.E., Smirnov V.Y., Maksimovich N.Ye., Semenenya I.N. Pool of free amino acids in the blood plasma of rats undergoing subtotal cerebral ischemia after L-name administration. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2019;16(2):185-191. (In Russ.) https://doi.org/10.29235/1814-6023-2019-16-2-185-191
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