OPTIMIZATION OF BASOPHIL ACTIVATION TEST CONDITIONS
Abstract
The basophil activation test (BAT) is a perspective method for allergy diagnosis. However, the optimization and standardization of the method are required. The optimal conditions for the peripheral blood storage for BAT were established: the blood should be stored at room temperature (18–25 °C), and a test should be performed within 4 h after blood collection. The optimal positive control – phorbol 12-myristate 13-acetate was chosen due to effective basophil activation and low cost. The expression of molecules on both unstimulated and activated basophils was studied. It was shown that CD11b, CD13, CD69, CD107a, CD164 and CD300a molecules may be used to improve the BAT efficiency.
About the Authors
I. U. Ramanava
The Republican Research and Practical Center for Epidemiology and Microbiology, Minsk
Belarus
Belarus
Researcher
23 Filimonov Str., 220114
A. Y. Hancharou
The Republican Research and Practical Center for Epidemiology and Microbiology, Minsk
Belarus
Belarus
Ph. D. (Med.), Head of the Laboratory
23, Filimonov Str., 220114
N. I. Dudarava
10th City Clinical Hospital, Minsk
Belarus
Belarus
Ph. D. (Med.), Head of the Department
73, Uborevich Str., 220096
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Review
For citations:
Ramanava I.U., Hancharou A.Y., Dudarava N.I. OPTIMIZATION OF BASOPHIL ACTIVATION TEST CONDITIONS. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2017;(4):48-59. (In Russ.)
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