Preview

Proceedings of the National Academy of Sciences of Belarus, Medical series

Advanced search

PROCARIOTIC IMMUNE SYSTEM: MOLECULAR MECHANISMS, APPLICATION IN MICROBIOLOGY

Abstract

During a short time, the CRISPR/Cas system has gained popularity among scientists in various fields of medicine, biology, and chemistry. Prokaryotes use the CRISPR/Cas system to protect their own genome from foreign genetic material, the cassette of which consists of Cas-protein coding genes, leader sequence, speisers and palindromes. Cas-proteins are the main thing without which the CRISPR/Cas system cannot work. While interacting with foreign DNA, the CRISPR/Cas system passes 3 stages: immunization, expression, and interference. Immunization takes place during the first bacterial contact with foreign DNA collecting information about an invasive agent. The next meeting reveals the protein complex creation and the foreign DNA destruction. The differences in the mechanisms of action depend on the system class and type. There are 2 classes of the CRISPR/Cas system separating into 5 types and 16 subtypes. Bioinformation methods are used to find the CRISPR/Cas system in a bacterial cell. Due to the high efficiency and the easy individual component assembly, scientists quickly learned how to benefit from the CRISPR/Cas system, applying it for a wide range of tasks. The CRISPR/Cas system is used in microbiology, genetics, molecular epidemiology, gene engineering, applied medicine, and pharmacology for genome editing, gene expression control, pathological process treatment and modeling, microorganism typing, determination of phylogenetic relationships of microorganisms and so on. The CRISPR/Cas system of N. meningitidis can successfully edit eucariotic genome. But experiments with human genome are connected with biomedical ethics.

For citations:


Kharkhal A.N., Titov L.P. PROCARIOTIC IMMUNE SYSTEM: MOLECULAR MECHANISMS, APPLICATION IN MICROBIOLOGY. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2017;(3):121-128. (In Russ.)

Views: 533


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1814-6023 (Print)
ISSN 2524-2350 (Online)