PARTICIPATION OF LIVER ARGINASE AND KUPFFER’S CELLS IN THE PROCESSES OF DETOXICATION AND DEVELOPMENT OF THE OXYDATIVE STRESS IN RATS WITH CHRONIC ETHANOL INTOXICATION

Abstract. Alcohol pathology is one of the most important problems of modern medicine. It is known that a high rate of morbidity and mortality caused by a regular use of alcoholic beverages is associated with toxic effects of ethanol on the most important human organs, primarily liver. To date, a sufficient number of facts have been accumulated, indicating the significance of Kupffer’s cells and liver arginase in the processes of life in normal and pathological conditions.The aim of the present investigation was to determine the liver arginase activity and the significance of Kupffer’s cells in the processes of detoxication and development of the oxydative stress in rats with chronic ethanol intoxication.1 In experiments on rats it was established that chronic ethanol intoxication is accompanied by a decrease in body temperature, liver arginase activity and by an increase in the level of «middle molecules», NO⁻₃ / NO⁻₂, lipide peroxidation products in plasma, the extent of blood toxicity, the activity of plasma alanineaminotransferase and aspartateaminotransferase, as well as in the duration of narcotic sleep. In the conditions of liver arginase depression by N^ω-hydroxy-nor-L-arginine, chronic alcoholization is accompanied by a more significant inhibition of the liver detoxication function, an increase in the content of NO⁻₃ / NO⁻₂ in plasma, lipid peroxidation products in the blood and liver and by a decrease in body temperature. Inhibition of the activity of Kupffer’s cells by gadolinium chloride reduces the toxic effect of ethanol on the liver, as well as on typical changes in the liver arginase activity, detoxification processes, and body temperature in rats with chronic ethanol intoxication.

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