GROWTH-STIMULATING ACTIVITY OF PLATELET PREPARATIONS IN RELATION TO MESENCHYMAL STEM CELLS IN VITRO

The goal of the study was to evaluate the effects of platelet lysate (PL) and platelet releatase (PR) on human mesenchymal stem cell (MSC) proliferation and apoptosis in vitro. As shown, the content of epidermal growth factor, basic fibroblast growth factor, but not transforming growth factor-β1 was 3 to 9 times higher in PL when compared to PR. The MSC proliferation in vitro for 7 days was equally increased 5 to 7 times by PL and PR generated at a platelet concentration of 5∙109 per ml. The PL/PR-mediated favourable effect on the MSC proliferation was potentiated by the presence of 5 % human AB serum when compared to 10 % fetal calf serum in the vulture medium. Both PL and PR generated at a platelet concentration of 10∙109 per ml decreased 2 to 4 times the level of early and late apoptotic cells after MSC culturing for 5 days. Generally, we concluded that PL and PR preparations are equal to potentiate the MSC growth in vitro for biotechnological purposes.

лизат тромбоцитов, релизат тромбоцитов, ростовые факторы, мезенхимальные стволовые клетки человека, пролиферация, апоптоз, platelet lysate, platelet releasate, human mesenchymal stem cells, proliferation, apoptosis

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