Integral evaluation of the antioxidant status and physical properties of membrane lipids of peripheral blood erythrocytes of patients with metabolic syndrome

Abstract

The status of the erythrocytes antioxidant system (AOS) and the plasma total antioxidant capacity (TAC) of patients with metabolic syndrome (MS), cerebral infarction. and discirculatory encephalopathy was investigated. We found the opposite changes in enzymes AOS activities (superoxide dismutase, glutathione peroxidase, catalase), a reduced glutathione level in erythrocytes, and a significant increase of plasma peripheral blood TAC of patients with the studied disorders compared to healthy donors. Using the lipophilic fluorescent probes, an increase of the lipid bilayer microviscosity of erythrocyte membranes of patients with MS was revealed. This testifies the changes of the physicochemical state of the cell membrane which probably is the result of lipid metabolism disturbance in the body. Thus, the study revealed the disbalance between antioxidants and prooxidants in red blood cells of patients with MS as a consequence of oxidative stress. The observed changes in the erythrocyte membranes lipid fluidity can be a cause of myocardial circulatory disorders detected with single-photon emission computed tomography.