Biological potential of a genetic engineering construction encoding the gene for the human vascular endothelium growth factor
https://doi.org/10.29235/1814-6023-2024-21-2-95-103
Abstract
The high biological potential of the developed genetically engineered plasmid construct pcDNATM3.1(–) VEGF165 has been established. It has been proven to induce a significant increase in the expression of the gene VEGF165 in mesenchymal multipotent stromal cells and human endothelial cells and to increase the production of the VEGF165 protein by cells. The identified temporary functional activity of the genetically engineered construct in cells and the absence of genotoxic effects minimize the likelihood of oncotransformation.
The created genetically engineered vector construct pcDNATM3.1(–) VEGF165 can be used to develop a gene therapy drug model that promotes angiogenesis in vivo in ischemic tissues.
Keywords
genetically engineered plasmid construction,
ischemia,
vascular endothelial growth factor,
VEGF165,
transfection,
gene expression
Supporting agencies: The work was carried out within the framework of a separate project for fundamental and applied research of the National Academy of Sciences of Belarus (No. GR 20230113).
About the Authors
V. G. Bogdan
Department of Medical Sciences of the National Academy of Sciences of Belarus
Belarus
Belarus
Vasiliy G. Bogdan – D. Sc. (Med.), Professor, Academician-Secretary
66, Nezavisimosti Ave., 220072, Minsk
А. G. Poleshko
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Anna G. Poleshko – Ph. D. (Biol.), Associate Professor, Head of the Laboratory
27, Akademicheskaya Str., 220072, Minsk
А. Yu. Misiukevich
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Ala Yu. Misiukevich – Researcher. Institute of Biophysics and Cell Engineering
27, Akademicheskaya Str., 220072, Minsk
А. А. Smirnov
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Andrei A. Smirnov – Junior Researcher
27, Akademicheskaya Str., 220072, Minsk
S. V. Sukhaveyeva
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Sviatlana V. Sukhaveyeva – Ph. D. (Biol.), Senior Researcher
27, Akademicheskaya Str., 220072, Minsk
А. V. Yantsevich
Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus
Belarus
Belarus
Aleksey V. Yantsevich – Ph. D. (Chem.), Associate Professor, Director
5-2, Akademicheskaya Str., 220072, Minsk
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Review
For citations:
Bogdan V.G., Poleshko А.G., Misiukevich А.Yu., Smirnov А.А., Sukhaveyeva S.V., Yantsevich А.V. Biological potential of a genetic engineering construction encoding the gene for the human vascular endothelium growth factor. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2024;21(2):95-103. (In Russ.) https://doi.org/10.29235/1814-6023-2024-21-2-95-103
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