Распределение частот полиморфизмов четырех генов цитокинов в здоровой популяции Западного Казахстана

Изучен ряд полиморфизмов генов, кодирующих IL-1, IL-6 и IL-10 и TNF-α ‒308, у 100 здоровых неродственных казахов с использованием ПЦР в реальном времени. Полученные результаты сравнивали с опубликованными данными по другим популяциям. Распределение генотипов соответствовало равновесию Харди-Вайнберга. Однако наблюдаемая частота генотипов цитокинов в казахской популяции показала сходство и различия по сравнению с соседними этническими группами. Наиболее заметные различия по сравнению с иранцами, турками, русскими и китайцами наблюдались в распределении генотипов IL-1, IL-6 и IL-10. Частота генотипа TNF-α ‒308 различалась только в иранской популяции. Таким образом, наше исследование показало связь между полиморфизмом генов цитокинов и этнической принадлежностью. Полученные нами результаты могут быть клинически значимы для понимания распространенности заболеваний в Казахстане.

1. Dinarello C. A. Historical insights into cytokines. European Journal of Immunology, 2007, vol. 37, suppl. 1, pp. 34−45. https://doi.org/10.1002/eji.200737772

2. Bhushan S., Perumal N. B. Disease associated cytokine SNPs database: an annotation and dissemination model. Cytokine, 2012, vol. 57, no. 1, pp. 107−112. https://doi.org/10.1016/j.cyto.2011.10.009

3. Baena A., Leung J. Y., Sullivan A. D., Landires I., Vasquez-Luna N., Quinones-Berrocal J. [et al.]. TNF-α promoter single nucleotide polymorphisms are markers of human ancestry. Genes and Immunity, 2002, vol. 3, no. 8, pp. 482–487. https://doi.org/10.1038/sj.gene.6363898

4. Chu W. M. Tumor necrosis factor. Cancer Letters, 2013, vol. 328, no. 2, pp. 222−225. https://doi.org/10.1016/j.canlet.2012.10.014

5. Jarkenov T. A., Skipenko O. G., Zhumabaev M. N., Zairova S. T., Akataev N. A., Yessenbaev D. B., Mukushev M. M., Nurmanov K. Zh., Balshambaev M. E. The association of tumor necrosis factor-α gene polymorphism with the severity of acute pancreatitis: a systematic review. Novosti khirurgii [Surgery news], 2020, vol. 28, no. 3, pp. 309–317. https://doi.org/10.18484/2305-0047.2020.3.309

6. Zabaleta J., Schneider B. G., Ryckman K., Hooper P. F., Camargo M. C., Piazuelo M. B. [et al.]. Ethnic differences in cytokine gene polymorphisms: potential implications for cancer development. Cancer Immunology, Immunotherapy, 2008, vol. 57, no. 1, pp. 107–114. https://doi.org/10.1007/s00262-007-0358-4

7. Amirzargar A., Sadeghi M., Khosravi F., Dianat S., Naroueynejad M., Nicknam M. H., Hatmi N., Ansaripour B., Moradi B., Nikbin B. Th1 and Th2 cytokine gene polymorphisms in two indigenous ethnic groups in Iran. International Journal of Immunogenetics, 2006, vol. 33, no. 6, pp. 429–437. https://doi.org/10.1111/j.1744-313X.2006.00636.x

8. Khosravi A., Javan B., Tabatabaiefar M. A., Ebadi H., Fathi D., Shahbazi M. Association of interleukin-1 gene cluster polymorphisms and haplotypes with multiple sclerosis in an Iranian population. Journal of Neuroimmunology, 2015, vol. 288, pp. 114–119. https://doi.org/10.1016/j.jneuroim.2015.09.009

9. Çelik Y., Dagli Ü., Kiliç M. Y., Törüner M., Özen S. C., Özkan M., Soykan I., Çetinkaya H., Ülker A., Özden A., Bozdayi A. M. Cytokine gene polymorphisms in Turkish patients with inflammatory bowel disease. Scandinavian Journal of Gastroenterology, 2006, vol. 41, no. 5, pp. 559−565. https://doi.org/10.1080/00365520500349523

10. Karaman E., Kucuk M. U., Bayramoglu A., Göçmen S. U., Ercan S., Guler H. I., Kucukkaya Y., Erden S. Investigation of relationship between IL-6 gene variants and hypertension in Turkish population. Cytotechnology, 2015, vol. 67, no. 6, pp. 947–954. https://doi.org/10.1007/s10616-014-9732-1

11. Samgina T. A., Bushueva O. Yu., Ivanov V. P., Solodilova M. A., Nazarenko P. M., Polonikov A. V. The association study of the promoter polymorphism ‒308G>A of tumor necrosis factor gene with the development and severity of acute pancreatitis in Russian population of Kursk region. Eksperimental’naya i klinicheskaya gastroenterologiya [Experimental and clinical gastroenterology], 2014, no. 9, pp. 17−20 (in Russian).

12. Samgina T. A., Zhivotova G. A., Nazarenko P. M., Polonikov A. V. The role of cytokine genetic polymorphism in development of acute pancreatitis: analysis of intergenic and environmental interactions. Rossiiskii zhurnal gastroenterologii, gepatologii, koloproktologii [Russian journal of gastroenterology, hepatology, coloproctology], 2017, vol. 27, no. 3, pp. 27‒33 (in Russian).

13. Wu S., Wang M., Wang Y., He J. Polymorphisms of cytokine genes and tuberculosis in two independent studies. Scientific Reports, 2019, vol. 9, no. 1, art. 2507. https://doi.org/10.1038/s41598-019-39249-4

14. Lu W., Pan K., Zhang L., Lin D., Miao X., You W. Genetic polymorphisms of interleukin (IL)-1B, IL-1RN, IL-8, IL-10 and tumor necrosis factor and risk of gastric cancer in a Chinese population. Carcinogenesis, 2005, vol. 26, no. 3, pp. 631−636. http://doi.org/10.1093/carcin/bgh349

15. van Dyke A. L., Cote M. L., Wenzlaff A. S., Land S., Schwartz A. G. Cytokine SNPs: Comparison of allele frequencies by race and implications for future studies. Cytokine, 2009, vol. 46, no. 2, pp. 236−244. https://doi.org/10.1016/j.jneuroim.2015.09.009

16. Kurdistani Z. K., Saberi S., Talebkhan Y., Oghalaie A., Esmaeili M., Mohajerani N. [et al.]. Distribution of cytokine gene single nucleotide polymorphisms among a multi-ethnic Iranian population. Advanced Biomedical Research, 2015, vol. 4, p. 160. https://doi.org/10.4103/2277-9175.161809

17. Alhamad E. H., Cal J. G., Shakoor Z., Almogren A. Cytokine gene polymorphisms of TNFα, IL-6, IL-10, TGFβ and IFNγ in the Saudi population. British Journal of Biomedical Science, 2013, vol. 70, no. 3, pp. 104−109. https://doi.org/10.1080/09674845.2013.11669944

18. Santovito A., Gendusa C., Matini A., Ferraro F., Musso I., Costanzo M., Delclos A., Cervella P. Frequency distribution of six cytokine gene polymorphisms in North- and South-Italy. International Journal of Immunogenetics, 2017, vol. 44, no. 4, pp. 158−163. https://doi.org/10.1111/iji.12324

19. Wainstein M. V., Mossmann M., Araujo G. N., Gonçalves S. C., Gravina G. L., Sangalli M. [et al.]. Elevated serum interleukin-6 is predictive of coronary artery disease in intermediate risk overweight patients referred for coronary angiography. Diabetology and Metabolic Syndrome, 2017, vol. 9, art. 67. https://doi.org/10.1186/s13098-017-0266-5

20. Zhu Z., Liu J. B., Liu X., Qian L. Association of interleukin 10 rs1800896 polymorphism with susceptibility to breast cancer: a meta-analysis. Journal of International Medical Research, 2020, vol. 48, no. 4, art. 300060520904863. https://doi.org/10.1177/0300060520904863

21. Ayelign B., Genetu M., Wondmagegn T., Adane G., Negash M., Berhane N. TNF-α (-308) gene polymorphism and type 2 diabetes mellitus in ethiopian diabetes patients. Diabetes, Metabolic Syndrome and Obesity, 2019, vol. 12, pp. 2453‒2459. https://doi.org/10.2147/DMSO.S229987