Experience of using the molecular genetic studies to assess the risk of suicide
https://doi.org/10.29235/1814-6023-2021-18-4-483-490
Abstract
In the recent decades, there has been widespread the opinion that genetic markers of the suicidal behavior (suicide, suicidal attempts, suicidal thoughts) can be used to predict the suicidal behavior.
The purpose of the study was to determine the possibility of using the method of molecular genetic research to assess the risk of suicide in men of 18‒27 years.
The study used the case-control method. The control group included 100 men of 18‒27 years who never had mental disorders. The suicide group included the persons who committed highly traumatic methods of self-harm and were motivated to commit suicide (30 persons). DNA isolation was performed using a NucleoSpin Blood kit (Macherey‒Nagel, Germany) according to the manufacturer’s protocol. Each DNA sample was analyzed for polymorphism by allelic discrimination using the real-time polymerase chain reaction (PCR).
The frequencies of occurrence of genotypes and alleles of the following genes were analyzed: HTR1A, rs6295 (G/C); BDNF, rs6265 (G/A); COMT, rs4680 (G/A); SKA2, rs7208505 (C/T); SLC6A4 (5HTT), rs25531 (T/C); 5HTR2A, rs6313 (G/A); TPH2, rs4570625 (G/T); TPH1, rs1800532 (G/T).
A statistically significant difference was found for the frequency of occurrence of genotypes and alleles of the rs25531 polymorphism of the SLC6A4 (5HTT) gene. The chance of being in the suicide group with a heterozygous genotype (T/C) carriage was 2.346 times higher.
The significance of the rs25531 polymorphism of the SLC6A4 (5HTT) gene for the formation of the suicidal behavior was confirmed.
About the Authors
S. V. Davidouski
Belarusian Medical Academy of Postgraduate Education
Belarus
Belarus
Siarhey V. Davidouski – Ph. D. (Med.), Associate Professor
3/3, P. Browka Str., 220013, Minsk
Zh. A. Ibragimova
Belarusian State Medical University
Belarus
Belarus
Zhanna A. Ibragimova – Ph. D. (Biol.), Head of the
Laboratory
83, Dzerzhinski Ave., 220116, Minsk, Republic of Belarus
A. S. Babenka
Belarusian State Medical University
Belarus
Belarus
Andrei S. Babenka ‒ Ph. D. (Chem.), Associate Professor
83, Dzerzhinski Ave., 220116, Minsk
M. M. Skuhareuskaya
Republican Scientific and Practical Center for Mental Health
Belarus
Belarus
Maryna M. Skuhareuskaya – D. Sc. (Med.), Associate
Professor, Head of the Department
220053, Minsk
S. A. Kastsiuk
Belarusian Medical Academy of Postgraduate Education
Belarus
Belarus
Sviatlana А. Kastsiuk ‒ D. Sc. (Med.), Professor, Head of the Laboratory
3/3, P. Browka Str., 220013, Minsk
S. I. Marchuk
Belarusian State Medical University
Belarus
Belarus
Svetlana I. Marchuk – Senior Researcher. Belarusian State Medical University
83, Dzerzhinski Ave., 220116, Minsk
Y. S. Davidouskaya
Belarusian State Medical University
Belarus
Belarus
Yanna S. Davidouskaya ‒ Researcher
83, Dzerzhinski Ave., 220116, Minsk
Yu. M. Mikitski
Republican unitary production enterprise “ACADEMPHARM”
Belarus
Belarus
Yuriy M. Mikitski – Director
5/3, Akademik V. F. Kuprevich Str., 220141, Minsk
Review
For citations:
Davidouski S.V., Ibragimova Zh.A., Babenka A.S., Skuhareuskaya M.M., Kastsiuk S.A., Marchuk S.I., Davidouskaya Y.S., Mikitski Yu.M. Experience of using the molecular genetic studies to assess the risk of suicide. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2021;18(4):483-490. https://doi.org/10.29235/1814-6023-2021-18-4-483-490
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