IMPLEMENTATION OF THE VITAL FUNCTIONS OF RATS AFTER THE REORGANIZATION OF THE NEURAL HIPPOCAMPUS CIRCUITRY BY MESENCHYMAL STEM CELLS
Abstract
At the Laboratory of Neurophysiology of the Institute of Physiology, National Academy of Sciences of Belarus the hypothesis was verified that one of the mechanisms of neuroplasticity with brain injury manifests itself in the natural activation of the process of migration of mesenchymal stem cells (MSCs). The protocol of obtaining and cultivating MSCs culture was worked out, evaluated their viability was evaluated, the possibility of using fluorescent markers for visualization of the migration processes was substantiated, the non-invasive ways to deliver MSCs to the injury site were analyzed.
In experiments on male rats it was demonstrated that the administration of mesenchymal stem cells in the sub-mucosal space of the nasal cavities of rats in 10 minutes after the unilateral destruction by the aspiration of 20 microlitre of the tissue CA1 of the hippocampus region is accompanied by the restoration of the vital functions and the protective and indicative reflexes as a whole by one week earlier than in the rats, into which MSCs were not administered after similar destructions of the brain in the sub-mucosal membrane of the nasal cavities.
In experiments on male rats it was demonstrated that the administration of mesenchymal stem cells in the sub-mucosal space of the nasal cavities of rats in 10 minutes after the unilateral destruction by the aspiration of 20 microlitre of the tissue CA1 of the hippocampus region is accompanied by the restoration of the vital functions and the protective and indicative reflexes as a whole by one week earlier than in the rats, into which MSCs were not administered after similar destructions of the brain in the sub-mucosal membrane of the nasal cavities.
About the Authors
S. G. Pashkevich
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Head of the Laboratory.
Belarus
Ph. D. (Biol.), Head of the Laboratory.
D. P. Tokalchik
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Junior researcher.
Belarus
Junior researcher.
G. P. Mironova
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Senior rereresearcher
Belarus
Ph. D. (Biol.), Senior rereresearcher
M. O. Dosina
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Senior rereresearcher
Belarus
Ph. D. (Biol.), Senior rereresearcher
O. G. Tichonovich
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Senior rereresearcher
Belarus
Ph. D. (Biol.), Senior rereresearcher
Yu. P. Stukach
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
Junior researcher.
Belarus
Junior researcher.
V. N. Kalyunov
Institute of Physiology of the National Academy of Sciences of Belarus
Belarus
D. Sc. (Biol.), Chief researcher.
Belarus
D. Sc. (Biol.), Chief researcher.
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Review
For citations:
Pashkevich S.G., Tokalchik D.P., Mironova G.P., Dosina M.O., Tichonovich O.G., Stukach Yu.P., Kalyunov V.N. IMPLEMENTATION OF THE VITAL FUNCTIONS OF RATS AFTER THE REORGANIZATION OF THE NEURAL HIPPOCAMPUS CIRCUITRY BY MESENCHYMAL STEM CELLS. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2017;(1):111-117. (In Russ.)
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