ROLE OF MESENCHYMAL STEM CELLS IN MAINTAINING THE VIABILITY AND FUNCTIONAL ACTIVITY OF HEPATOCYTE CULTURE IN VITRO

The research results of the influence of mesenchymal stem cells (MSCs) isolated from adipose tissue on the viability and the functional activity in the co-culture of hepatocytes. It was established that the method of culturing hepatocytes isolated from the liver does not provide a larger number of these cells and leads to decrease in the viability from 98 to 15  % on the 1st day and on the 7th day, respectively. Synthesis of liver secretory proteins HGF and ANGPTL4 demonstrates the possibility of using MSCs as the substrate to maintain the functional properties of isolated hepatocytes during cultivation. Therefore, the co-culturing of adipose tissue-derived mesenchymal stem cells and isolated liver cells promotes cell transplant preservation for a certain period of time as compare with the monoculture of hepatocytes. The auxiliary role of mesenchymal stem cells is particularly promising in the context of cell transplantation for acute liver failure, in which the co-transplantation of MSC and hepatocytes may provide an optimal combination of the functional activity of liver support with anti-inflammatory action.

hepatocytes, mesenchymal stem cells, co-culture, level of liver secretory soluble proteins, morphological characteristics, cell transplantation

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