Characteristics of immune status in patients after liver transplantation with mesenchymal stem cell therapy
https://doi.org/10.29235/1814-6023-2025-22-3-220-231
Abstract
The aim of the study was evaluation of the characteristics of the immunophenotype of peripheral blood mononuclear cells in patients after liver transplantation with systemic mesenchymal stem cells (MSCs) therapy. A randomized prospective study was conducted involving 30 recipients. Patients in the MSC group (n = 15) received standard immunosuppressive therapy in combination with systemic application of MSCs, and patients in the control group (n = 15) received only standard immunosuppression. The analysis of the immunophenotype of peripheral blood mononuclear cells was performed by flow cytometry with assessment of the main populations and subpopulations of lymphocytes and dendritic cells. The application of MSCs resulted in an immunotolerant phenotype, characterized by an increase in the level of regulatory T cells and B1a lymphocytes, a decrease in the number of effector CD4+ memory T cells and B lymphocytes, as well as a modulation of dendritic cells activity. The MSC group demonstrated a lower incidence of acute rejection (20 % compared to 33 %), lower expression of MMP10 in the graft, accelerated recovery of liver function, and the ability to reduce the dose of Tacrolimus without the risk of rejection. These results indicate the potential of MSCs to serve as an additional method of immunosuppression in liver transplantation
About the Authors
S. V. Korotkov
Minsk Scientific and Practical Center for Surgery,
Transplantology and Hematology
Belarus
Belarus
Sergey V. Korotkov – Ph. D. (Med.), Associate Professor.
8, Semashko Str., 220045, Minsk, Republic of Belarus
V. V. Smolnikova
Minsk Scientific and Practical Center for Surgery,
Transplantology and Hematology
Russian Federation
Russian Federation
Viktoriya V. Smolnikova – Ph. D. (Biol.), Leading Researcher
8, Semashko Str., 220045, Minsk, Republic of Belarus
V. Yu. Grinevich
Minsk Scientific and Practical Center for Surgery,
Transplantology and Hematology
Russian Federation
Russian Federation
Viktoriya Yu. Grinevich – Head of the Laboratory
8, Semashko Str., 220045, Minsk, Republic of Belarus
A. E. Shcherba
Minsk Scientific and Practical Center for Surgery,
Transplantology and Hematology
Russian Federation
Russian Federation
Aleksey E. Shcherba – D. Sc. (Med.), Professor
8, Semashko Str., 220045, Minsk, Republic of Belarus
S. I. Krivenko
Minsk Scientific and Practical Center for Surgery,
Transplantology and Hematology
Russian Federation
Russian Federation
Svetlana I. Krivenko – D. Sc. (Med.), Professor
8, Semashko Str., 220045, Minsk, Republic of Belarus
O. O. Rummo
Minsk Scientific and Practical Center for Surgery,
Transplantology and Hematology
Belarus
Belarus
Oleg O. Rummo – Academician, D. Sc. (Med.), Professor,
Director
8, Semashko Str., 220045, Minsk, Republic of Belarus
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Review
For citations:
Korotkov S.V., Smolnikova V.V., Grinevich V.Yu., Shcherba A.E., Krivenko S.I., Rummo O.O. Characteristics of immune status in patients after liver transplantation with mesenchymal stem cell therapy. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2025;22(3):220-231. (In Russ.) https://doi.org/10.29235/1814-6023-2025-22-3-220-231
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