vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2024-21-1-62-67vestim-954Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEОценка метаболизма коллагенов при экспериментальном БЦЖ-индуцированном туберкулезном воспаленииAssessing the collagen metabolism in experimental BCG-induced tuberculous inflammationhttps://orcid.org/0000-0001-9599-3049ПутятинаА. Н.PutyatinaA. N.

Путятина Анна Николаевна – канд. мед. наук, науч. сотрудник

ул. Тимакова, 2, 630117, г. Новосибирск

Anna N. Putyatina – Ph. D. (Med.), Researcher

2, Timakov Str., 630117, Novosibirsk

putyatina@ngs.ruhttps://orcid.org/0000-0002-4051-8854КимЛ. Б.KimL. B.

Ким Лена Борисовна – д-р мед. наук, гл. науч. сотрудник

ул. Тимакова, 2, 630117, г. Новосибирск

Lena B. Kim – D. Sc. (Med.), Chief Researcher, Head of the group

2, Timakov Str., 630117, Novosibirsk

lbkim@frcftm.ruhttps://orcid.org/0000-0003-1565-5248РусскихГ. С.RusskikhG. S.

Русских Галина Сергеевна – канд. биол. наук, ст. науч. сотрудник

ул. Тимакова, 2, 630117, г. Новосибирск

Galina S. Russkikh – Ph. D. (Biol.), Senior Researcher

2, Timakov Str., 630117, Novosibirsk

russkikh_g@mail.ruФедеральный исследовательский центр фундаментальной и трансляционной медициныFederal Research Center of Fundamental and Translational Medicine2024040320242116267Copyright © Путятина А.Н., Ким Л.Б., Русских Г.С., 20242024Путятина А.Н., Ким Л.Б., Русских Г.С.Putyatina A.N., Kim L.B., Russkikh G.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/954

В работе представлены данные, полученные при исследовании органов мышей с БЦЖ-индуцированным гранулематозом. В печени, легких, селезенке мышей опытной и контрольной групп измеряли содержание фракций свободного, пептидно-связанного, белково-связанного гидроксипролина (ГОП) методом щелочного гидролиза в собственной модификации. У мышей с БЦЖ-гранулематозом отмечено повышенное содержание фракций ГОП, отражающих синтез коллагенов, тогда как содержание свободного ГОП, свидетельствующего о деградации коллагенов, не отличалось от аналогичных показателей контрольной группы. Анализ соотношения отдельных фракций ГОП продемонстрировал различную степень выраженности фиброза в органах мышей: усиленный – в легких, умеренный – в печени, слабый – в селезенке, что свидетельствует об органоспецифичности фиброгенеза при туберкулезной инфекции.

The paper presents the study results of the organs of mice without (control group) and with BCG-induced granulomatosis. The contents of hydroxyproline (Hyp) fractions (free, peptide-bound, and protein-bound) were measured in the liver, lungs, and spleen by our alkaline hydrolysis method. In mice with BCG-induced granulomatosis, an increased content of Hyp fractions, reflecting the collagen synthesis, was observed, while the content of free Hyp, characterizing the collagen degradation, did not differ from similar indicators of the control group. The analysis of the ratios of individual Hyp fractions showed a different fibrosis degree in the organs. It was high in the lungs, moderate in the liver and weak in the spleen, thus indicating the organ-specific fibrogenesis in tuberculous infection.

фракции гидроксипролинаколлагенщелочной гидролизфиброзтуберкулезорганы мышейhydroxyproline fractionscollagenalkaline hydrolysisfibrosistuberculosisorgans of miceReferencesHydroxyproline as a biomarker in liver disease / S. A. Gabr [et al.] // Biomarkers in liver disease. Biomarkers in disease: methods, discoveries and applications. ‒ Dordrecht, 2016. – P. 471–491. https://doi.org/10.1007/978-94-007-7742-2_26-1Gabr S. A., Alghadir A. H., Sherif Y. E., Ghfar A. A. Hydroxyproline as a biomarker in liver disease. Biomarkers in liver disease. Biomarkers in disease: methods, discoveries and applications. Dordrecht, 2016, pp. 471–491. https://doi.org/10.1007/978-94-007-7742-2_26-1The collagen suprafamily: from biosynthesis to advanced biomaterial development / A. Sorushanova [et al.] // Adv. Mater. – 2019. – Vol. 31, N 1. – P. e1801651. https://doi.org/10.1002/adma.201801651Sorushanova A., Delgado L. M., Wu Z., Shologu N., Kshirsagar A., Raghunath R. [et al.]. The collagen suprafamily: from biosynthesis to advanced biomaterial development. Advanced Materials, 2019, vol. 31, no. 1, p. e1801651. https://doi.org/10.1002/adma.201801651Extracellular matrix markers and methods for their study (review) / E. V. Tush [et al.] // CTM. – 2019. – Vol. 11, N 2. – P. 133–147. https://doi.org/10.17691/stm2019.11.2.20Tush E. V., Eliseeva Т. I., Khaletskaya О. V., Krasilnikova S. V., Ovsyannikov D. Yu., Potemina T. E., Ignatov S. K. Extracellular matrix markers and methods for their study (review). CTM, 2019, vol. 11, no. 2, pp. 133–147. https://doi.org/10.17691/stm2019.11.2.20Siddiqi, N. J. Effect of sodium fluoride and magnesium chloride on different hydroxyproline fractions in rat liver / N. J. Siddiqi // J. Biochem. Biophys. – 2012. – Vol. 49, N 2. – P. 130–133.Siddiqi N. J. Effect of sodium fluoride and magnesium chloride on different hydroxyproline fractions in rat liver. Journal of Biochemistry and Biophysics, 2012, vol. 49, no. 2, pp. 130–133.Measurement of hydroxyproline in collagen with three different methods / B. Qiu [et al.] // Mol. Med. Rep. – 2014. – Vol. 10, N 2. – P. 1157–1163. https://doi.org/10.3892/mmr.2014.2267Qiu B., Wei F., Sun X., Wang X., Duan B., Shi C., Zhang J., Zhang J., Qiu W., Mu W. Measurement of hydroxyproline in collagen with three different methods. Molecular Medicine Reports, 2014, vol. 10, no. 2, pp. 1157–1163. https://doi.org/10.3892/mmr.2014.2267da Silva, C. M. L. Fast and sensitive collagen quantification by alkaline hydrolysis/hydroxyproline assay / C. M. L. da Silva, E. Spinelli, S. V. Rodrigues// Food Chem. – 2015. – Vol. 173. – P. 619–623. https://doi.org/10.1016/j.foodchem.2014.10.073da Silva C. M. L., Spinelli E., Rodrigues S. V. Fast and sensitive collagen quantification by alkaline hydrolysis/ hydroxyproline assay. Food Chemistry, 2015, vol. 173, pp. 619–623. https://doi.org/10.1016/j.foodchem.2014.10.073Assaying for total collagen content. Articular cartilage / K. A. Athanasiou [et al.]. ‒ 2nd ed. ‒ Boca Raton, 2017. – P. 585–587.Athanasiou K. A., Darling E. M., Hu J. C., Reddi A. H. Assaying for total collagen content. Articular cartilage. 2nd ed. Boca Raton, 2017, pp. 585–587.Dynamics of aldosterone, connective tissue reorganization and glucose level as markers for tuberculosis treatment effectiveness / O. S. Shevchenko [et al.] // Archives Balkan Med. Union. – 2019. – Vol. 54, N 2. – P. 274–280. https://doi.org/10.31688/ABMU.2019.54.2.08Shevchenko O. S., Todoriko L. D., Ovcharenko I. A., Radzishevska Ye. B., Shvets O. M., Ovcharenko S. S., Semianiv I. O., Vivsyannuk V. V. Dynamics of aldosterone, connective tissue reorganization and glucose level as markers for tuberculosis treatment effectiveness. Archives of the Balkan Medical Union, 2019, vol. 54, no. 2, pp. 274–280. https://doi.org/10.31688/ABMU.2019.54.2.08Способ определения фракций гидроксипролина в биологическом материале : пат. № 2735375 от 30.10.2020 / А. Н. Путятина, Г. С. Русских, Л. Б. Ким // Изобретения. Полезные модели: офиц. бюл. – 2020. – № 31.Putyatina A. N., Russkikh G. S., Kim L. B. Method of determining fractions of hydroxyproline in biological material. Patent no. 2735375 (30.10.2020). Izobreteniya. Poleznye modeli: ofitsial’nyi byulleten’ [Inventions. Utility models: official bulletin], 2020, no. 31 (in Russian).Content of the major extracellular matrix components of the liver and lung in mice with chronic BCG-granulomatosis treated with liposome-encapsulated dextrazide / L. B. Kim [et al.] // Bull. Exp. Biol. Med. – 2021. – Vol. 170, N 4. – P. 453– 457. https://doi.org/10.1007/s10517-021-05086-7Kim L. B., Putyatina A. N., Russkikh G. S., Shkurupy V. A. Content of the major extracellular matrix components of the liver and lung in mice with chronic BCG-granulomatosis treated with liposome-encapsulated dextrazide. Bulletin of Experimental Biology and Medicine, 2021, vol. 170, no. 4, pp. 453–457. https://doi.org/10.1007/s10517-021-05086-7Fibrogenesis in granulomas and lung interstitium in tuberculous inflammation in mice / V. A. Shkurupiy [et al.] // Bull. Exp. Biol. Med. – 2014. – Vol. 156, N 6. – P. 731–735. https://doi.org/10.1007/s10517-014-2435-yShkurupiy V. A., Kim L. B., Potapova O. V., Cherdantseva L. A., Putyatina A. N., Nikonova I. K. Fibrogenesis in granulomas and lung interstitium in tuberculous inflammation in mice. Bulletin of Experimental Biology and Medicine, 2014, vol. 156, no. 6, pp. 731–735. https://doi.org/10.1007/s10517-014-2435-yJayasankar, K. Biochemical and histochemical changes relating to fibrosis following infection with Mycobacterium tuberculosis in the guinea pig / K. Jayasankar, V. D. Ramanathan // Indian J. Med. Res. – 1999. – Vol. 110. – P. 91–97.Jayasankar K., Ramanathan V. D. Biochemical and histochemical changes relating to fibrosis following infection with Mycobacterium tuberculosis in the guinea pig. Indian Journal of Medical Research, 1999, vol. 110, pp. 91–97.Lung gene expression signatures suggest pathogenic links and molecular markers for pulmonary tuberculosis, adenocarcinoma and sarcoidosis / Q. Chai [et al.] // Commun. Biol. – 2020. – Vol. 3, N 1. – P. 604. https://doi.org/10.1038/s42003-020-01318-0Chai Q., Lu Z., Liu Z., Zhong Y., Zhang F., Qiu C., Li B., Wang J., Zhang L., Pang Y., Liu C. H. Lung gene expression signatures suggest pathogenic links and molecular markers for pulmonary tuberculosis, adenocarcinoma and sarcoidosis. Communications Biology, 2020, vol. 3, no. 1, p. 604. https://doi.org/10.1038/s42003-020-01318-0Тарасова, Л. Г. Особенности коллагенового обмена в сопоставлении с иммунологическим статусом у больных туберкулезом легких / Л. Г. Тарасова, Е. Н. Стрельцова // Астрахан. мед. журн. – 2016. – Т. 11, № 4. – С. 100–105.Tarasova L. G., Strel’tsova E. N. Features of collagen exchange as compared to immunological status of patients with pulmonary tuberculosis. Astrahanskii meditsinskii zhurnal [Astrakhan medical journal], 2016, vol. 11, no. 4, pp. 100–105 (in Russian).Экспрессия генов белков, сопряженных с фибротическими процессами, в легких мышей при развитии туберкулезного воспаления / П. М. Кожин [и др.] // Сибир. науч. мед. журн. – 2019. – Т. 39, № 4. – С. 22–29.Kozhin P. M., Chechushkov A. V., Zaitseva N. S., Khrapova M. V., Cherdantseva L. A., Men’shchikova E. B., Troitskii A. V., Shkurupii V. A. Expression of protein genes participating in fibroplastic processes in mice lung during the development of tuberculous inflammation. Sibirskii nauchnyi meditsinskii zhurnal [Siberian scientific medical journal], 2019, vol. 39, no. 4, pp. 22–29 (in Russian).

The authors declare that there are no conflicts of interest present.