vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2024-21-1-43-52vestim-952Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEТриптофан и цинк: влияние совместного введения на гомеостаз свободных аминокислот плазмыTryptophan and zinc: effect of co-administration on plasma free amino acid homeostasisШейбакВ. М.SheybakV. M.

Шейбак Владимир Михайлович ‒ д-р мед. наук, профессор, заведующий кафедрой

ул. Виленская, 19, 230023, г. Гродно

Vladimir M. Sheybak ‒ D. Sc. (Med.), Professor, Head of the Department

80, Gorky Str., 230015, Grodno

vsheibak@gmail.comhttps://orcid.org/0000-0001-7995-5587ПавлюковецА. Ю.PavlyukovetsA. Yu.

Павлюковец Анастасия Юрьевна – канд. биол. наук, доцент

ул. Виленская, 19, 230023, г. Гродно

Anastasia Yu. Pavlyukovets – Ph. D. (Biol.), Associate Professor

80, Gorky Str., 230015, Grodno

anastasiayk@mail.ruДорошенкоЕ. М.DoroshenkoE. M.

Дорошенко Евгений Михайлович – канд. биол. наук, доцент, вед. науч. сотрудник

(ул. Виленская, 19, 230023, г. Гродно

Evgeniy M. Doroshenko – Ph. D. (Biol.), Associate Professor, Leading Researcher

80, Gorky Str., 230015, Grodno

dgi03@mail.ruГродненский государственный медицинский университетGrodno State Medical University2024040320242114352Copyright © Шейбак В.М., Павлюковец А.Ю., Дорошенко Е.М., 20242024Шейбак В.М., Павлюковец А.Ю., Дорошенко Е.М.Sheybak V.M., Pavlyukovets A.Y., Doroshenko E.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/952

Одна из основных гидрофобных аминокислот – триптофан и незаменимый микроэлемент цинк вы­полняют в организме млекопитающих многочисленные и во многом перекрывающиеся функции. Огромное количе­ство цинксодержащих соединений (белки, ферменты, факторы транскрипции и гормоны) взаимодействуют с мета­болитами триптофана. Нами предпринята попытка выявить основные эффекты аминокислоты и микроэлемента на показатели метаболизма аминокислот с целью определения общих механизмов и целесообразности их возможного терапевтического использования.

Нами обнаружено, что при курсовом введении триптофана в дозе 40 мг/кг в плазме крови снижается общее ко­личество АРУЦ и повышается концентрация свободного триптофана. У животных, получавших цинка диаспартат или цинка диаспартат совместно с триптофаном, снижалось общее количество аминокислот и их азотсодержащих метаболитов в плазме крови. Анализ индивидуальных концентраций аминокислот и их азотсодержащих метаболи­тов показал, что совместное введение триптофана и цинка диаспартата сопровождается статистически значимыми изменениями концентраций большинства изучаемых показателей аминокислотного пула (19 из 35 показателей). Сле­дует отметить однонаправленность изменений содержания свободных аминокислот и их азотсодержащих метаболи­тов в группах животных, получавших цинка диаспартат или цинка диаспартат совместно с триптофаном (совпаде­ние эффектов составляет 90 %).

Таким образом, проведенные нами исследования показали, что курсовое введение цинка диаспартата в дозе 25 мг/кг массы (в 2 раза превышает среднюю терапевтическую дозу) оказывает выраженный метаболический эф­фект, характеризующийся изменениями концентраций свободных аминокислот и их азотсодержащих метаболитов в плазме крови. Курсовое введение триптофана в дозе 40 мг/кг массы (1/2 терапевтической дозы) не оказывает суще­ственного влияния на показатели аминограммы плазмы крови. При совместном введении цинка диаспартата и трип­тофана в указанных дозах выявленные изменения в большей степени можно отнести к эффектам цинка диаспартата. 

One of the main hydrophobic amino acids – tryptophan and essential trace element zinc perform numerous and largely overlapping functions in the mammalian body. A huge number of zinc-containing compounds – proteins, en­zymes, transcription factors, and hormones interact with tryptophan metabolites. We have attempted to identify the main effects of amino acids and trace elements on amino acid metabolism in order to determine the general mechanisms and the feasibility of their possible therapeutic use.

We have found that the course administration of tryptophan at a dose of 40 mg/kg in blood plasma reduces the total amount of ARUC and increases the concentration of free tryptophan. In animals treated with zinc diaspartate or zinc diaspar­tate together with tryptophan, the total amount of amino acids and their nitrogen-containing metabolites in blood plasma de­ creases. The analysis of individual concentrations of amino acids and their nitrogen-containing metabolites showed that the coadministration of tryptophan and zinc diaspartate is accompanied by statistically significant changes in the concentrations of most of the studied parameters of the amino acid pool (19 out of 35 parameters). It should be noted that the changes in the content of free amino acids and their nitrogen-containing metabolites are unidirectional in the groups of animals treated with zinc diaspartate or zinc diaspartate together with tryptophan (coincidence of effects is 90 %). 

Thus, our studies showed that the course administration of zinc diaspartate at a dose of 25 mg/kg (2 times higher than an average therapeutic dose) has a pronounced metabolic effect, characterized by changes in the concentrations of free amino acids and their nitrogen-containing metabolites in blood plasma. The course administration of tryptophan at a dose of 40 mg/ kg of body weight (1/2 of a therapeutic dose) has no significant effect on the parameters of the blood plasma aminogram. With the coadministration of zinc diaspartate and tryptophan in the above doses, the revealed changes can be more attributed to the effects of zinc diaspartate.

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The authors declare that there are no conflicts of interest present.