vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2022-19-2-219-229vestim-846Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEУплыў кверцэтына і яго камбінацыі з цыкладэкстрынам на загойванне паўнаслойных скурных ран у лабараторных пацукоўEffect of quercetin and its combination with cyclodextrin on the healing of full-thickness skin wounds in laboratory ratsБакуновічА. А.BakunovichA. A.

Бакуновіч Аляксей Аляксеевіч – малодшы навуковы супрацоўнік

БЛК, 50, 230030, г. Гродна

Aliaksei A. Bakunovich  – Junior Researcher

50, BLK, 230030, Grodno

baptist.alexei@mail.ruБукоВ. У.BukoV. U.

Буко Вячаслаў Ульянавіч – доктор біялагічных навук, прафесар, загадчык аддзелам

БЛК, 50, 230030, г. Гродна

Vyachaslau U. Buko  – D. Sc. (Biol.), Professor, Head of the Department

50, BLK, 230030, Grodno

vu.buko@tut.byСідаровічА. А.SidarovichA. A.

Сідаровіч Алена Анатольеўна – асістэнт

вул. Горкага, 80, 230009, г. Гродна

Alena A. Sidarovich – Assistant

80, Gorky Str., 230009, Grodno

elenasidm@rambler.ruАстроўскаяА. Б.AstrowskayaA. B.

Астроўская Аксана Барысаўна – кандыдат біялагічных навук, дацэнт, старэйшы навуковы супрацоўнік

вул. Горкага, 80, 230009, г. Гродна

Aksana B. Astrowskaja – Ph. D. (Biol.), Associate Professor, Senior Researcher

80, Gorky Str., 230009, Grodno

astrowskaja@gmail.comМарозВ. Л.MarozV. L.

Мароз Валянціна Леапольдаўна  – навуковы супрацоўнік

БЛК, 50, 230030, г. Гродна

Valiantsina L. Maroz  – Researcher

50, BLK, 230030, Grodno

valmor655@gmail.comЯрашэнкаЮ. У.YarashenkaYu. V.

Ярашэнка Юлія Уладзіміраўна – малодшы навуковы супрацоўнік

БЛК, 50, 230030, г. Гродна

Yulia V. Yarashenka  – Junior Researcher

50, BLK, 230030, Grodno

julia2222@yandex.ruАстроўскіА. А.AstrowskiA. A.

Астроўскі Аляксандр Аляксандравіч – доктар медыцынскіх навук, прафесар, вядучы навуковы супрацоўнік

БЛК, 50, 230030, г. Гродна

Aliaksandr A. Astrowski  – D. Sc. (Med.), Professor, Leading Researcher

50, BLK, 230030, Grodno

astrowski@gmail.comІнстытут біяхіміі біялагічна актыўных злучэнняў НАН БеларусіInstitute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of BelarusГродзенскі дзяржаўны медыцынскі ўніверсітэтGrodno State Medical University202208062022192219229Copyright © Бакуновіч А.А., Буко В.У., Сідаровіч А.А., Астроўская А.Б., Мароз В.Л., Ярашэнка Ю.У., Астроўскі А.А., 20222022Бакуновіч А.А., Буко В.У., Сідаровіч А.А., Астроўская А.Б., Мароз В.Л., Ярашэнка Ю.У., Астроўскі А.А.Bakunovich A.A., Buko V.U., Sidarovich A.A., Astrowskaya A.B., Maroz V.L., Yarashenka Y.V., Astrowski A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/846

З дапамогай макраскапічнага, гісталагічнага, электронна-мікраскапічнага, мікрабіялагічнага ды імуналагічнага метадаў вывучана загойванне паўнаслойных скурных ран у лабараторных пацукоў пад уплывам кверцэтына (КВ) і яго комплекса з 2-гідроксіпрапіл-β-цыкладэкстрынам. Пад уздзеяннем КВ рана цалкам пакрывалася эпідэрмісам амаль на суткі раней, чым у кантролі, а пад уздзеяннем комплекса з 2-гідроксіпрапіл-β-цыкладэкстрынам ‒ на 2,2 сут раней (p < 0,02). Гэта адбывалася пераважна за кошт паскарэння краявой эпідэрмізацыі. Пры гэтым пазітыўнага ўплыву ўзгаданых вышэй рэчываў на кантракцыю раны не выяўлена. КВ і асабліва яго комплекс з цыкладэкстрынам аказалі стымулюючы ўплыў на фагацытарную і метабалічную актыўнасць нейтрафілаў як на паверхні раны, так і ў крыві жывёл. Бактэрыяльная мікрафлора, якая прысутнічала на ранавай паверхні падчас загойвання, супадала з мікрафлорай інтактнай скуры. Ні КВ, ні кверцэтын-2-гідроксіпрапіл-β-цыкладэкстрын істотна не паўплывалі на яе склад.

Macroscopic, histological, electron microscopic, microbiological, and immunological methods were used to study the healing of full-thickness skin wounds in laboratory rats under the influence of quercetin and its complex with 2-hydroxypropyl-β-cyclodextrin. It was revealed that under the influence of quercetin, the wound was completely covered with the epidermis almost a day earlier than in the control, and under the influence of a complex with 2-hydroxypropylβ-cyclodextrin 2.2 days earlier (p < 0.02). This was mainly due to the acceleration of marginal epidermization. There was no positive effect on the wound contraction. Quercetin and especially its complex with cyclodextrin had a stimulating effect on the phagocytic and metabolic activity of neutrophils both on the wound surface and in the blood of animals. The bacterial microflora present on the wound surface during healing coincided with the microflora of the intact skin. Neither the quercetin, nor the quercetin-2-hydroxypropyl-β-cyclodextrin significantly affected its composition.

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The authors declare that there are no conflicts of interest present.