vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2022-19-1-62-69vestim-826Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEЭкспрессия генов Fasn и Sirt4 и кодируемых ими белков в висцеральной жировой ткани крыс линии Вистар при избыточном потреблении жиров животного происхожденияExpression of the Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intakeПолуляхО. Е.PoluliakhO. Y.

Полулях Ольга Евгеньевна - научный сотрудник.

ул. Академическая, 28, 220072, Минск.

Olga Y. Poluliakh – Researcher, Institute of Physiology of the National Academy of Sciences of Belarus.

28, Akademicheskaya Str., 220072, Minsk.

reanzy@yandex.ruКалиновскаяЕ. И.KalinovskayaE. I.

Калиновская Елена Игоревна - кандидат медицинских наук, ведущий научный сотрудник.

ул. Академическая, 28, 220072, Минск.

Elena I. Kalinovskaya - Ph. D. (Med.), Leading Researcher, Institute of Physiology of the National Academy of Sciences of Belarus.

28, Akademicheskaya Str., 220072, Minsk.

zolotuhinaelena@mail.ruБасалайА. А.BasalaiA. A.

Басалай Анастасия Александровна - научный сотрудник.

ул. Академическая, 28, 220072, Минск.

Anastasia A. Basalai – Researcher, Institute of Physiology of the National Academy of Sciences of Belarus.

28, Akademicheskaya Str., 220072, Minsk.

anastasiya.basalay@gmail.comМигалевичА. С.MihalevichA. S.

Мигалевич Анастасия Сергеевна - младший научный сотрудник.

ул. Академическая, 28, 220072, Минск.

Anastasia S. Mihalevich - Junior Researcher, Institute of Physiology of the National Academy of Sciences of Belarus.

28, Akademicheskaya Str., 220072, Minsk.

mihalevich.a.s@mail.ruИнститут физиологии НАН БеларусиInstitute of Physiology of the National Academy of Sciences of Belarus2022100320221916269Copyright © Полулях О.Е., Калиновская Е.И., Басалай А.А., Мигалевич А.С., 20222022Полулях О.Е., Калиновская Е.И., Басалай А.А., Мигалевич А.С.Poluliakh O.Y., Kalinovskaya E.I., Basalai A.A., Mihalevich A.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/826

Представлены результаты собственных исследований изменения экспрессии липогенных генов Fasn и Sirt4 и кодируемых ими белков Fasn и Sirt4 в висцеральной жировой ткани крыс линии Вистар при избыточном потреблении жиров.

В течение 8 недель дополнительно к стандартному рациону вивария крысы получали жиры животного происхождения (45 % от суточной калорийности). Относительную экспрессию генов определяли методом ПЦР в режиме реального времени, содержание белков в висцеральной жировой ткани - методом иммуноферментного анализа.

Установлено, что при избыточном потреблении жиров животного происхождения в висцеральной жировой ткани крыс линии Вистар снижается экспрессия липогенных генов Fasn и Sirt4 и кодируемых ими белков, что свидетельствует о дисфункции жировой ткани, следствием чего может быть нарушение метаболизма липидов и углеводов, развитие инсулинорезистентности.

The changes in the expression of the lipogenic Fasn and Sirt4 genes and the Fasn and Sirt4 proteins they encode in the visceral adipose tissue of Wistar rats against the background of excess fat intake were studied.

In addition to the standard vivarium diet, the rats received the animal fats (45 % of daily caloric value) for 8 weeks. A relative gene expression was determined by real-time PCR, protein content in the visceral adipose tissue - by the ELISA method.

It was found that the excess animal fat intake leads to a decreased expression of lipogenic Fasn and Sirt4 genes and the proteins they encode in the visceral adipose tissue of Wistar rats, which indicates the formation of the adipose tissue dysfunction, which may result in the impared lipid and carbohydrate metabolism, the insulin resistance development.

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The authors declare that there are no conflicts of interest present.