vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2022-19-1-38-47vestim-820Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEЭффективность дистантного ишемического посткондиционирования миокарда у крыс с индуцированным метаболическим синдромом зависит от уровня лептинаThe efficiency of remote ischemic postconditioning of the myocardium in rats with induced metabolic syndrome depends on the leptin levelНарыжнаяН. В.NaryzhnayaN. V.

Нарыжная Наталья Владимировна - доктор медицинских наук, ведущий научный сотрудник.

ул. Киевская, 111А, 634012, Томск.

Natalia V. Naryzhnaya - D. Sc. (Med.), Leading Researcher, Cardiology Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences.

111А, Kievskaya Str., 634012, Tomsk.

natalynar@yandex.ruЛогвиновС. В.LogvinovS. V.

Логвинов Сергей Валентинович - доктор медицинских наук, профессор, заведующий кафедрой, НИИ кардиологии Томского НИМЦ РАН; СибГМУ.

ул. Киевская, 111А, 634012, Томск; Московский тракт, 2, 634050, Томск.

Sergey V. Logvinov - D. Sc. (Med.), Professor, Head of the Department, Cardiology Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences; Siberian State Medical University.

111А, Kievskaya Str., 634012, Tomsk; 2, Moskovski trakt, 634050, Tomsk.

s_logvinov@mail.ruКурбатовБ. К.KurbatovB. K.

Курбатов Борис Константинович - младший научный сотрудник.

ул. Киевская, 111А, 634012, Томск.

Boris K. Kurbatov - Junior Researcher, Cardiology Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences.

111А, Kievskaya Str., 634012, Tomsk.

bobersanker@gmail.comМухомедзяновА. В.MukhomedzyanovA. V.

Мухомедзянов Александр Валерьевич - кандидат медицинских наук, младший научный сотрудник.

ул. Киевская, 111А, 634012, Томск.

Alexandr V. Mukhomedzyanov - Ph. D. (Med.), Junior Researcher, Cardiology Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences.

111А, Kievskaya Str., 634012, Tomsk.

sasha_m91@mail.ruСиротинаМ. А.SirotinaM. A.

Сиротина Мария Александровна - аспирант.

ул. Киевская, 111А, 634012, Томск.

Maria A. Sirotina - Postgraduate student, Cardiology Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences.

111А, Kievskaya Str., 634012, Tomsk.

sirotina_maria@mail.ruЧепелевС. Н.ChepelevS. N.

Чепелев Сергей Николаевич - старший преподаватель.

пр. Дзержинского, 83, 220116, Минск.

Sergey N. Chepelev - Senior Lecturer, Belarusian State Medical University.

83, Dzerzhinski Ave., 220116, Minsk.

drserge1991@gmail.comВисмонтФ. И.VismontF. I.

Висмонт Франтишек Иванович - член-корреспондент, доктор медицинских наук, профессор, заведующий кафедрой.

пр. Дзержинского, 83, 220116, Минск.

Frantishek I. Vismont - Corresponding Member, D. Sc. (Med.), Professor, Head of the Department, Belarusian State Medical University.

83, Dzerzhinski Ave., 220116, Minsk.

patfiz@bsmu.byМасловЛ. Н.MaslovL. N.

Маслов Леонид Николаевич - доктор медицинских наук, профессор, руководитель лаборатории.

ул. Киевская, 111А, 634012, Томск.

Leonid N. Maslov - D. Sc. (Med.), Professor, Head of the Laboratory, Cardiology Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences.

111А, Kievskaya Str., 634012, Tomsk.

maslov@cardio-tomsk.ruНаучно-исследовательский институт кардиологии Томского национального исследовательского медицинского центра РАНCardiology Research Institute of Tomsk National Research Medical Center of the Russian Academy of SciencesНаучно-исследовательский институт кардиологии Томского национального исследовательского медицинского центра РАН; Сибирский государственный медицинский университетCardiology Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences; Siberian State Medical UniversityБелорусский государственный медицинский университетBelarusian State Medical University2022100320221913847Copyright © Нарыжная Н.В., Логвинов С.В., Курбатов Б.К., Мухомедзянов А.В., Сиротина М.А., Чепелев С.Н., Висмонт Ф.И., Маслов Л.Н., 20222022Нарыжная Н.В., Логвинов С.В., Курбатов Б.К., Мухомедзянов А.В., Сиротина М.А., Чепелев С.Н., Висмонт Ф.И., Маслов Л.Н.Naryzhnaya N.V., Logvinov S.V., Kurbatov B.K., Mukhomedzyanov A.V., Sirotina M.A., Chepelev S.N., Vismont F.I., Maslov L.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/820

Дистантное посткондиционирование (ДПост) имеет большой терапевтический потенциал в плане защиты миокарда при ишемии-реперфузии. При этом при наличии у пациента метаболических нарушений клиническое применение кондиционирующих воздействий ограничено.

Цель настоящей работы - оценить влияние индуцированного метаболического синдрома (иМетС) на инфаркт-лимитирующий эффект дистантного ишемического посткондиционирования у крыс и изучить механизмы этого влияния. Исследование проведено на крысах линии Вистар. Для индуцирования метаболического синдрома (МетС) крыс содержали на высокоуглеводной высокожировой диете. Критериями развития МетС считали увеличение массы животного, объема абдоминального жира, развитие артериальной гипертензии, гиперхолестеринемии, гиперлептинемии, гипергликемии, повышение содержания триглицеридов в сыворотке крови, развитие состояния инсулинорезистентности по значимому повышению индекса Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) и нарушение толерантности к глюкозе. Всем животным моделировали 45-минутную коронароокклюзию и 120-минутную реперфузию.

ДПост приводило к двукратному сокращению размера инфаркта у крыс с интактным метаболизмом (р < 0,0001), в то время как у крыс с иМетС уменьшение размера инфаркта при ДПост составило 25 % (р = 0,00003), что было значимо ниже, чем у животных без иМетС (р < 0,0001). Выявлена прямая корреляционная зависимость размера инфаркта при ДПост от содержания лептина в сыворотке крови крыс с иМетС.

Анализ полученных данных позволяет сделать предположение о том, что снижение эффективности ДПост у крыс с индуцированным диетой МетС зависит от содержания лептина в крови.

Remote postconditioning (RPost) has a great therapeutic potential for protecting the myocardium during ischemiareperfusion in clinical practice. At the same time, an important problem limiting the use of conditioning effects in the clinic is the presence of metabolic disorders in the patient. The aim of this work was to assess the effect of induced metabolic syndrome (iMetS) on the efficacy of the infarct-limiting effect of remote ischemic postconditioning (RPost) in rats and to study the mechanisms of this effect.

The study was carried out on Wistar rats. MetS was induced by high-carbohydrate high-fat diet. Criteria of metabolic syndrome were an increase in the weight of animals, abdominal fat volume, the development of arterial hypertension, hypercholesterolemia, an increase in triglycerides in serum, hyperleptinemia, hyperglycemia, the development of a state of insulin resistance by a significant increase in the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index and glucose tolerance. All animals were subjected to 45 min coronary occlusion and 120 min reperfusion.

RPost led to a twofold reduction of infarct size in rats with intact metabolism (р < 0.0001), while in rats with iMetS a decrease in infarct size during RPost was 25 % (p = 0.00003), which was significantly lower than in animals without iMetC (р < 0.0001). A direct correlation was found between of infarct size during RPost and the serum leptin level of rats with iMetC.

The presented data suggested that a decrease in the efficiency of remote postconditioning in rats with diet-induced metabolic syndrome depends on leptin content in blood.

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The authors declare that there are no conflicts of interest present.