vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2021-18-4-483-490vestim-806Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEВопыт выкарыстання малекулярна-генетычных даследаванняў для ацэнкі рызыкi самагубстваExperience of using the molecular genetic studies to assess the risk of suicideДавідоўскіС. У.DavidouskiS. V.

Siarhey V. Davidouski – Ph. D. (Med.), Associate Professor

3/3, P. Browka Str., 220013, Minsk

davidouski@yandex.ruІбрагімаваЖ. А.IbragimovaZh. A.

Zhanna A. Ibragimova  – Ph. D. (Biol.), Head of theLaboratory

83, Dzerzhinski Ave., 220116, Minsk, Republic of Belarus

lbmibgmu@mail.ruБабенкаА. С.BabenkaA. S.

Andrei S. Babenka ‒ Ph. D. (Chem.), Associate Professor

83, Dzerzhinski Ave., 220116, Minsk

lbmibgmu@mail.ruСкугарэўскаяМ. М.SkuhareuskayaM. M.

Maryna M. Skuhareuskaya  – D. Sc. (Med.), AssociateProfessor, Head of the Department

220053, Minsk

marims@tut.byКасцюкС. А.KastsiukS. A.

Sviatlana А. Kastsiuk ‒ D. Sc. (Med.), Professor, Head of the Laboratory

3/3, P. Browka Str., 220013, Minsk

s.kostuk@mail.ruМарчукС. I.MarchukS. I.

Svetlana I. Marchuk  – Senior Researcher. Belarusian State Medical University

83, Dzerzhinski Ave., 220116, Minsk

lbmibgmu@mail.ruДавідоўскаяЯ. С.DavidouskayaY. S.

Yanna S. Davidouskaya ‒ Researcher

83, Dzerzhinski Ave., 220116, Minsk

davidouskaia@gmail.comМікіцкіЮ. М.MikitskiYu. M.

Yuriy M. Mikitski – Director

5/3, Akademik V.  F.  Kuprevich Str., 220141, Minsk

mikitski@mail.ruBelarusian Medical Academy of Postgraduate EducationBelarusBelarusian State Medical UniversityBelarusRepublican Scientific and Practical Center for Mental HealthBelarusRepublican unitary production enterprise “ACADEMPHARM”Belarus202104122021184483490Copyright © Давідоўскі С.У., Ібрагімава Ж.А., Бабенка А.С., Скугарэўская М.М., Касцюк С.А., Марчук С.I., Давідоўская Я.С., Мікіцкі Ю.М., 20212021Давідоўскі С.У., Ібрагімава Ж.А., Бабенка А.С., Скугарэўская М.М., Касцюк С.А., Марчук С.I., Давідоўская Я.С., Мікіцкі Ю.М.Davidouski S.V., Ibragimova Z.A., Babenka A.S., Skuhareuskaya M.M., Kastsiuk S.A., Marchuk S.I., Davidouskaya Y.S., Mikitski Y.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/806

У апошнія дзесяцігоддзі шырока распаўсюдзілася меркаванне, што генетычныя маркеры суіцыдальных паводзін (суіцыд, суіцыдальныя спробы, суіцыдальныя думкі) можна выкарыстоўваць для ацэнкi рызыкi самагубства.

Мэта даследавання  ‒ ацаніць магчымасць выкарыстання метаду малекулярна-генетычнага даследавання для ацэнкі рызыкі самагубства.

У даследаванні выкарыстоўваўся метад кантролю за выпадкамі. У кантрольную группу ўваходзілі 100 чалавек 18‒27 гадоў, якія ніколі не мелі псіхічных разладаў, у групу самагубцаў  ‒ 30 чалавек, якія здзейснілі траўматычныя метады самапашкоджання з выражаным намерам здейснiць самагубства. Вылучэнне ДНК праводзілася з выкарыстаннем набору NucleoSpin Blood (Macherey-Nagel, Нямеччына) у адпаведнасці з пратаколам вытворцы. Кожны ўзор ДНК аналізавалі на палімарфізм шляхам алельнай дыскрымінацыі з выкарыстаннем ланцуговай рэакцыі палімеразы ў рэжыме рэальнага часу. Былі прааналізаваны частоты ўзнікнення генатыпаў і алеляў наступных генаў: HTR1A, rs6295 (G/C); BDNF, rs6265 (G/A); COMT, rs4680 (G/A); SKA2, rs7208505 (C/T); SLC6A4 (5HTT), rs25531 (T/C); 5HTR2A, rs6313 (G/A); TPH2, rs4570625 (G/T); TPH1, rs1800532 (G/T).

Выяўлена статыстычна значная розніца ў частаце ўзнікнення генатыпаў і алеляў палімарфізму rs25531 гена SLC6A4 (5HTT). Шанцы апынуцца ў групе самагубцаў гетэразіготнага генатыпу (Т/С) былі ў 2,346 разы вышэй.

Пацверджана значэнне полімарфізму rs25531 гена SLC6A4 (5HTT) для фарміравання суіцыдальных паводзін

In the recent decades, there has been widespread the opinion that genetic markers of the suicidal behavior (suicide, suicidal attempts, suicidal thoughts) can be used to predict the suicidal behavior.

The purpose of the study was to determine the possibility of using the method of molecular genetic research to assess the risk of suicide in men of 18‒27 years.

The study used the case-control method. The control group included 100 men of 18‒27 years who never had mental disorders. The suicide group included the persons who committed highly traumatic methods of self-harm and were motivated to commit suicide (30 persons). DNA isolation was performed using a NucleoSpin Blood kit (Macherey‒Nagel, Germany) according to the manufacturer’s protocol. Each DNA sample was analyzed for polymorphism by allelic discrimination using the real-time polymerase chain reaction (PCR).

The frequencies of occurrence of genotypes and alleles of the following genes were analyzed: HTR1A, rs6295 (G/C); BDNF, rs6265 (G/A); COMT, rs4680 (G/A); SKA2, rs7208505 (C/T); SLC6A4 (5HTT), rs25531 (T/C); 5HTR2A, rs6313 (G/A); TPH2, rs4570625 (G/T); TPH1, rs1800532 (G/T).

A statistically significant difference was found for the frequency of occurrence of genotypes and alleles of the rs25531 polymorphism of the SLC6A4 (5HTT) gene. The chance of being in the suicide group with a heterozygous genotype (T/C) carriage was 2.346 times higher.

The significance of the rs25531 polymorphism of the SLC6A4 (5HTT) gene for the formation of the suicidal behavior was confirmed.

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The authors declare that there are no conflicts of interest present.