vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2021-18-4-402-412vestim-798Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEУровни миокина иризин и адипоцитокинов в сыворотке крови у маловесных доношенных детей при рожденииBlood serum myokine irisin and adipocytokine levels in newborns small for gestational age at birthПрилуцкаяВ. А.PrylutskayaV. A.

Прилуцкая Вероника Анатольевна – канд. мед. наук, доцент.

пр. Дзержинского, 83, 220116, г. Минск

Verаnika A. Prylutskaya – Ph. D. (Med.), Associate Professor

83, Dzerzhinski Ave., 220116, Minsk

2489861@rambler.ruСукалоА. В.SukaloA. V.

Сукало Александр Васильевич – академик, д-р мед. наук, профессор, заведующий кафедрой

пр. Дзержинского, 83, 220116, г. Минск

Alexander V. Sukalo – Academician, D. Sc. (Med.), Professor, Head of the Department

83, Dzerzhinski Ave., 220116, Minsk

childill1@bsmu.byГончарикА. В.GoncharikA. V.

Гончарик Антонина Викторовна – cт. науч. сотрудник

пр. Дзержинского, 83, 220116, г. Минск, Республика Беларусь

Antonina V. Goncharik – Senior Researcher

83, Dzerzhinski Ave., 220116,Minsk

goncharik-t@mail.ruБелорусский государственный медицинский университетBelarusian State Medical University202103122021184402412Copyright © Прилуцкая В.А., Сукало А.В., Гончарик А.В., 20212021Прилуцкая В.А., Сукало А.В., Гончарик А.В.Prylutskaya V.A., Sukalo A.V., Goncharik A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/798

В последние годы проведен целый ряд исследований миокина иризин у взрослых и единичные ‒ у новорожденных. Показана роль адипоцитокинов в процессах роста и развития плода и детей.

Цель исследования ‒ оценить уровни миокина иризин и адипоцитокинов у маловесных к сроку гестации доношенных детей при рождении и проанализировать взаимосвязи параметров гормонального статуса детей и их матерей.

Проведено обследование 49 новорожденных и их матерей. Выделено две группы детей: группа 1 (Гр1) ‒ маловесные к сроку гестации (n = 24), группа 2 (Гр2) ‒ соответствующие сроку гестации (n = 25). Уровни иризина и адипоцитокинов сыворотки крови определяли иммуноферментным методом.

Установлено, что у маловесных новорожденных значимо более низкие уровни лептина и ИФР-1 в пуповинной крови, чем у детей, физическое развитие которых соответствует гестационному возрасту. У новорожденных Гр1 и Гр2 не выявлено значимых различий в содержании иризина в сыворотке пуповинной крови. Обнаружены значимые положительные корреляционные взаимосвязи между уровнем иризина пуповинной крови маловесных новорожденных и массой тела при рождении. В Гр1 выявлена положительная связь между уровнями иризина матерей и детей (r = 0,518, р = 0,028). Установлено, что различия в содержании иризина зависят от способа родоразрешения (p = 0,0104).

Выявленные статистически значимые различия в уровнях анализируемых метаболических маркеров в парах мать‒дитя, их взаимосвязи с клинико-антропометрическими параметрами обосновывают возможность применения иризина и адипоцитокинов в качестве предикторов при прогнозировании формирования нарушений обменных процессов у маловесных к сроку гестации детей.

In recent years, a number of the studies of myokine irisin in adults and isolated in newborns have been carried out. The role of adipocytokines in the growth and development of the fetus and children has been shown.

The aim of the study was to assess the levels of myokine irisin and adipocytokines in newborns small for gestational age at birth and to analyze the relationship between the parameters of the hormonal status of children and their mothers.

49 newborns and their mothers were examined. Two groups were identified: group 1 (Gr1) – newborns small for gestational age (n = 24), group 2 (Gr2) – newborns appropriate for gestational age (n = 25). The levels of irisin and adipocytokines in the blood serum were determined by the enzyme immunoassay.

Newborns small for gestational age had significantly lower levels of leptin and IGF-1 in the umbilical cord blood compared to children with physical development corresponding to the gestational age. There were no significant differences in the irisin content of cord blood serum in newborn Gr1 compared with Gr2. The presence of significant positive correlations between the level of irisin in the umbilical cord blood of newborns small for gestational age and the body weight at birth was established. In Gr1, a positive relationship was found between the irisin levels of mothers and newborns (r = 0.518, p = 0.028). The differences in the irisin content between the groups were established, taking into account the delivery mode (p = 0.0104).

The revealed statistically significant differences in the concentrations of the analyzed metabolic markers in mother–child pairs, their relationship with clinical and anthropometric parameters substantiate the possibility of using irisin and adipocytokines as predictors in predicting the formation of metabolic disorders of infants small for gestational age.

иризинмиокиныноворожденныемаловесный к сроку гестацииадипоцитокинылептинвисфатинмасса теламатериirisinmyokinesnewbornssmall for gestational ageadipocytokinesleptinvisfatinbody weightmothersРабота выполнена в рамках задания «Изучить биохимические и клинико-антропометрические показатели маловесных и крупновесных новорожденных с нарушениями ранней адаптации» Государственной программы научных исследований «Фундаментальные и прикладные науки – медицине», № госрегистрации 20200275.Acknowledgеments. The work was carried out as part of the task “To study the biochemical and clinical-anthropometric indicators of low-weight and large-weight newborns with early adaptation disorders” of the State Program of Scientific Research “Fundamental and Applied Sciences ‒ Medicine”, state registration No. 20200275.ReferencesЦориев, Т. Т. Роль миокинов в межтканевом взаимодействии и регуляции обмена веществ: обзор литературы / Т. Т. Цориев, Ж. Е. Белая, Л. Я. Рожинская // Остеопороз и остеопатии. ‒ 2016. ‒ Т. 19, № 1. ‒ С. 28‒34.Tsoriev T. T., Belaya Zh. E., Rozhinskaya L. Ya. The role of myokines in interstitial interaction and regulation of metabolism: a review of the literature. Osteoporoz i osteopatii [Osteoporosis and bone diseases], 2016, vol. 19, no. 1, pp. 28‒34 (in Russian).Panati, K. Irisin/FNDC5 ‒ an updated review / K. Panati, Y. Suneetha, V. R. Narala // Eur. Rev. Med. Pharmacol. Sci. ‒ 2016. ‒ Vol. 20, N 4. ‒ P. 689‒697.Panati K., Suneetha Y., Narala V. R. Irisin/FNDC5 ‒ an updated review. European Review for Medical and Pharmacological Sciences, 2016, vol. 20, no. 4, pp. 689‒697.A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis / P. Boström [et al.] // Nature. ‒ 2012. ‒ Vol. 481, N 7382. ‒ P. 463‒468. https://doi.org/10.1038/nature10777Boström P., Wu J., Jedrychowski M. P., Korde A., Ye L., Lo J. C. [et al.]. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature, 2012, vol. 481 (7382), pp. 463‒468. https://doi.org/10.1038/ nature10777Roca-Rivada, A. FNDC5/irisin is not only a myokine but also an adipokine / A. Roca-Rivada, C. Castelao, L. L. Senin // PLoS ONE. ‒ 2013. ‒ Vol. 8, N 4. ‒ P. e60563. https://doi.org/10.1371/journal.pone.0060563Roca-Rivada A., Castelao C., Senin L. L. FNDC5/irisin is not only a myokine but also an adipokine. PLoS ONE, 2013, vol. 8, no. 4, p. e60563. https://doi.org/10.1371/journal.pone.0060563Keleş, E. Evaluation of cord blood irisin levels in term newborns with small gestational age and appropriate gestational age / E. Keleş, F. F. Turan // SpringerPlus. ‒ 2016. ‒ Vol. 5, N 1. ‒ P. 1757. https://doi.org/10.1186/s40064-016-2869-yKeleş E., Turan F. F. Evaluation of cord blood irisin levels in term newborns with small gestational age and appropriate gestational age. SpringerPlus, 2016, vol. 5, no. 1, p. 1757. https://doi.org/10.1186/s40064-016-2869-yArhire, L. I. Irisin: a hope in understanding and managing obesity and metabolic syndrome / L. I. Arhire, L. Mihalache, M. Covasa // Front. Endocrinol. ‒ 2019. ‒ Vol. 10. ‒ P. 524. https://doi.org/10.3389/fendo.2019.00524Arhire L. I., Mihalache L., Covasa M. Irisin: a hope in understanding and managing obesity and metabolic syndrome. Frontiers in Endocrinology, 2019, vol. 10, p. 524. https://doi.org/10.3389/fendo.2019.00524FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise / J. Y. Huh [et al.] // Metabolism. ‒ 2012. ‒ Vol. 61, N 12. ‒ P. 1725‒1738. https://doi.org/10.1016/j.metabol.2012.09.002Huh J. Y., Panagiotou G., Mougios V., Brinkoetter M., Vamvini M. T., Schneider B. E., Mantzoros C. S. FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism, 2012, vol. 61, no. 12, pp. 1725‒1738. https://doi. org/10.1016/j.metabol.2012.09.002Maternal serum and fetal cord blood irisin levels in gestational diabetes mellitus / M. A. Yuksel [et al.] // Diabetes Res. Clin. Pract. ‒ 2014. ‒ Vol. 104, N 1. ‒ P. 171‒175. https://doi.org/10.1016/j.diabres.2013.12.025Yuksel M. A., Oncul M., Tuten A., Imamoglu M., Acikgoz A. S., Kucur M., Madazli R. Maternal serum and fetal cord blood irisin levels in gestational diabetes mellitus. Diabetes Research and Clinical Practice, 2014, vol. 104, no. 1, pp. 171‒175. https://doi.org/10.1016/j.diabres.2013.12.025Irisin levels during pregnancy and changes associated with the development of preeclampsia / M. F. Garcés [et al.] // J. Clin. Endocrinol. Metab. ‒ 2014. ‒ Vol. 99, N 6. ‒ P. 2113‒2119. https://doi.org/10.1210/jc.2013-4127Garcés M. F., Peralta J. J., Ruiz-Linares C. E., Lozano A. R., Poveda N. E., Torres-Sierra A. L. [et al.]. Irisin levels during pregnancy and changes associated with the development of preeclampsia. Journal of Clinical Endocrinology and Metabolism, 2014, vol. 99, no. 6, pp. 2113‒2119. https://doi.org/10.1210/jc.2013-4127Al-Ghazali, M. J. Serum irisin levels as a potential marker for diagnosis of gestational diabetes mellitus / M. J. AlGhazali, H. A. Ali, M. M. Al-Rufaie // Acta Biomed. ‒ 2020. ‒ Vol. 19, N 91 (1). ‒ P. 56‒63. https://doi.org/10.23750/abm. v91i1.7675Al-Ghazali M. J., Ali H. A., Al-Rufaie M. M. Serum irisin levels as a potential marker for diagnosis of gestational diabetes mellitus. Acta Biomedica, 2020, vol. 91, no. 1, pp. 56‒63. https://doi.org/10.23750/abm.v91i1.7675Irisin in idiopathic foetal growth restriction / M. Çağlar [et al.] // J. Endocrinol. Invest. ‒ 2014. ‒ Vol. 37, N 7. ‒ P. 619‒624. https://doi.org/10.1007/s40618-014-0078-5Çağlar M., Göksu M., Isenlik B. S., Yavuzcan A., Yılmaz M., Üstün Y., Aydin S., Kumru S. Irisin in idiopathic foetal growth restriction. Journal of Endocrinological Investigation, 2014, vol. 37, no. 7, pp. 619‒624. https://doi.org/10.1007/ s40618-014-0078-5Cord blood irisin levels are positively correlated with birth weight in newborn infants / K. E. Joung [et al.] // Metabolism. ‒ 2015. ‒ Vol. 64, N 11. ‒ P. 1507‒1514. https://doi.org/10.1016/j.metabol.2015.07.019Joung K. E., Park K. H., Filippaios A., Dincer F., Christou H., Mantzoros C. S. Cord blood irisin levels are positively correlated with birth weight in newborn infants. Metabolism, 2015, vol. 64, no. 11, pp. 1507‒1514. https://doi.org/10.1016/j. metabol.2015.07.019Circulating irisin in relation to insulin resistance and the metabolic syndrome / K. H. Park [et al.] // J. Clin. Endocrinol. Metab. ‒ 2013. ‒ Vol. 98, N 12. ‒ P. 4899‒4907. https://doi.org/10.1210/jc.2013-2373Park K. H., Zaichenko L., Brinkoetter M., Thakkar B., Sahin-Efe A., Joung K. E. [et al.]. Circulating irisin in relation to insulin resistance and the metabolic syndrome. Journal of Clinical Endocrinology and Metabolism, 2013, vol. 98, no. 12, pp. 4899‒4907. https://doi.org/10.1210/jc.2013-2373Evaluation of irisin and visfatin levels in very low birth weight preterm newborns compared to full term newborns ‒ a prospective cohort study / N. Mól [et al.] // PLoS ONE. ‒ 2018. ‒ Vol. 13, N 9. ‒ P. e0204835. https://doi.org/10.1371/journal. pone.0204835Mól N., Zasada M., Tomasik P., Klimasz K., Kwinta P. Evaluation of irisin and visfatin levels in very low birth weight preterm newborns compared to full term newborns ‒ a prospective cohort study. PLoS ONE, 2018, vol. 13, no. 9, p. e0204835. https://doi.org/10.1371/journal.pone.0204835Insulin resistance in short children with intrauterine growth retardation / P. L. Hofman [et al.] // J. Clin. Endocrinol. Metab. ‒ 1997. ‒ Vol. 82, N 2. ‒ P. 402‒406. https://doi.org/10.1210/jcem.82.2.3752Hofman P. L., Cutfield W. S., Robinson E. M., Bergman R. N., Menon R. K., Sperling M. A., Gluckman P. D. Insulin resistance in short children with intrauterine growth retardation. Journal of Clinical Endocrinology and Metabolism, 1997, vol. 82, no. 2, pp. 402‒406. https://doi.org/10.1210/jcem.82.2.3752Современные представления о роли адипоцитокинов в программировании гормонально-метаболических процессов у маловесных к сроку гестации детей / А. В. Сукало [и др.] // Педиатрия. Восточная Европа. − 2015. − № 1 (09). ‒ С. 130–141.Sukalo A. V., Prilutskaya V. A., Solntseva A. V., Uvarova E. V. Modern views on the role of adipocytokines in programming hormonal and metabolic processes in small for gestational age children. Pediatriya. Vostochnaya Evropa [Pediatrics. Eastern Europe], 2015, no. 1 (09), pp. 130–141 (in Russian).Barker, D. J. The origins of the developmental origins theory / D. J. Barker // J. Int. Med. ‒ 2007. ‒ Vol. 261, N 5. ‒ P. 412‒417. https://doi.org/10.1111/j.1365-2796.2007.01809.xBarker D. J. The origins of the developmental origins theory. Journal of Internal Medicine, 2007, vol. 261, no. 5, pp. 412‒417. https://doi.org/10.1111/j.1365-2796.2007.01809.xCoelho, M. Biochemistry of adipose tissue: an endocrine organ / M. Coelho, T. Oliveira, R. Fernandes // Arch. Med. Sci. ‒ 2013. ‒ Vol. 9, N 2. ‒ P. 191–200. https://doi.org/10.5114/aoms.2013.33181Coelho M., Oliveira T., Fernandes R. Biochemistry of adipose tissue: an endocrine organ. Archives of Medical Science, 2013, vol. 9, no. 2, pp. 191–200. https://doi.org/10.5114/aoms.2013.33181Münzberg, H. Structure, production and signaling of leptin / H. Münzberg, C. D. Morrison // Metabolism. ‒ 2015. ‒ Vol. 64, N 1. ‒ P. 13–23. https://doi.org/10.1016/j.metabol.2014.09.010Münzberg H., Morrison C. D. Structure, production and signaling of leptin. Metabolism, 2015, vol. 64, no. 1, pp. 13–23. https://doi.org/10.1016/j.metabol.2014.09.010Cord blood adipocytokines and body composition in early childhood: a systematic review and meta-analysis / C. Bagias [et al.] // Int. J. Environ. Res. Public Health. ‒ 2021. ‒ Vol. 18, N 4. ‒ P. 1897. https://doi.org/10.3390/ijerph18041897Bagias C., Sukumar N., Weldeselassie Y., Oyebode O., Saravanan P. Cord blood adipocytokines and body composition in early childhood: a systematic review and meta-analysis. International Journal of Environmental Research and Public Health, 2021, vol. 18, no. 4, p. 1897. https://doi.org/10.3390/ijerph18041897Maternal visfatin concentration in normal pregnancy / S. Mazaki-Tovi [et al.] // J. Perinat. Med. ‒ 2009. ‒ Vol. 37, N 3. ‒ P. 206–217. https://doi.org/10.1515/JPM.2009.054Mazaki-Tovi S., Romero R., Kusanovic J. P., Vaisbuch E., Erez O., Than N. G. [et al.]. Maternal visfatin concentration in normal pregnancy. Journal of Perinatal Medicine, 2009, vol. 37, no. 3, pp. 206–217. https://doi.org/10.1515/JPM.2009.054Programming of adiposity in childhood and adolescence: associations with birth weight and cord blood adipokines / J. Simpson [et al.] // J. Clin. Endocrinol. Metab. ‒ 2017. ‒ Vol. 102, N 2. ‒ P. 499–506. https://doi.org/10.1210/jc.2016-2342Simpson J., Smith A. D., Fraser A., Sattar N., Lindsay R. S., Ring S. M., Tilling K., Davey S. G., Lawlor D. A., Nelson S. M. Programming of adiposity in childhood and adolescence: associations with birth weight and cord blood adipokines. Journal of Clinical Endocrinology and Metabolism, 2017, vol. 102, no. 2, pp. 499–506. https://doi.org/10.1210/jc.2016-2342The Global Health network [Electronic resource]. – Mode of access: https://intergrowth21.tghn.org/standards-tools/. – Date of access: 05.06.2021.The Global Health network [Electronic resource]. Available at: https://intergrowth21.tghn.org/standards-tools/ (accessed June 5, 2021).The role of irisin, insulin and leptin in maternal and fetal interaction / D. Ökdemir [et al.] // J. Clin. Res. Pediatr. Endocrinol. ‒ 2018. ‒ Vol. 10, N 4. ‒ P. 307‒315. https://doi.org/10.4274/jcrpe.0096Ökdemir D., Hatipoğlu N., Kurtoğlu S., Siraz Ü. G, Akar H. H., Muhtaroğlu S., Kütük M. S. The role of irisin, insulin and leptin in maternal and fetal interaction. Journal of Clinical Research in Pediatric Endocrinology, 2018, vol. 10, no. 4, pp. 307‒315. https://doi.org/10.4274/jcrpe.0096Прилуцкая, В. А. Іризин: новий міокін та його вплив на обмін речовин / В. А. Прилуцкая, А. В. Солнцева, Т. А. Піскун // Укр. журн. дит. ендокринол. – 2021. – № 1. – С. 17–28.Prylutskaya V. A., Solntsаva A. V., Piskun T. A. Irizin: a new myokine and its effect on metabolism. Ukrainskii zhurnal dityachii endokrinologii [Ukrainian journal of pediatric endocrinology], 2021, no. 1, pp. 17–28 (in Ukrainian).Cord blood irisin at the extremes of fetal growth / S. Baka [et al.] // Metabolism. ‒ 2015. ‒ Vol. 64, N 11. ‒ P. 1515‒1520. https://doi.org/10.1016/j.metabol.2015.07.020Baka S., Malamitsi-Puchner A., Boutsikou T., Boutsikou M., Marmarinos A., Hassiakos D., Gourgiotis D., Briana D. D. Cord blood irisin at the extremes of fetal growth. Metabolism, 2015, vol. 64, no. 11, pp. 1515‒1520. https://doi.org/10.1016/j.metabol.2015.07.020

The authors declare that there are no conflicts of interest present.