vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2021-18-3-351-361vestim-786Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEНаночастицы серебра: экологичный метод синтеза, свойства и использование против антибиотикорезистентной микрофлорыSilver nanoparticles: an ecological method of synthesis, properties and use against antibiotic resistant microfloraДовнарР. И.DovnarR. I.

Довнар Руслан Игоревич – канд. мед. наук, доцент

ул. Горького, 80, 230009, г. Гродно

Ruslan I. Dovnar – Ph. D. (Med.), Associate Professor

80, Gorky Str., 230009, Grodno 

dr_ruslan@mail.ruВасильковА. Ю.Vasil’kovA. Yu.

Васильков Александр Юрьевич – канд. хим. наук, доцент, вед. науч. сотрудник

ул. Вавилова, 28, 119991, г. Москва

Alexander Yu. Vasil’kov – Ph. D. (Chem.), Associate Professor, Leading Researcher

28, Vavilov Str., 119991, Moscow 

alexandervasilkov@yandex.ruСоколоваТ. Н.SakalovaT. M.

Соколова Татьяна Николаевна – канд. мед. наук, доцент

ул. Горького, 80, 230009, г. Гродно

Tatsiana M. Sakalova – Ph. D. (Med.), Associate Professor

80, Gorky Str., 230009, Grodno 

sakalova@tut.byНаумкинА. В.NaumkinA. V.

Наумкин Александр Васильевич – канд. физ.-мат. наук, ст. науч. сотрудник

ул. Вавилова, 28, 119991, г. Москва

Alexander V. Naumkin – Ph. D. (Phys. and Math.), Senior Researcher

28, Vavilov Str., 119991, Moscow 

naumkin@ineos.ac.ruБудниковА. В.BudnikovA. V.

Будников Александр Викторович – мл. науч. cотрудник

ул. Вавилова, 28, 119991, г. Москва

Alexander V. Budnikov – Junior Researcher

28, Vavilov Str., 119991, Moscow 

budnikow501@ineos.ac.ruДовнарИ. С.DovnarI. S.

Довнар Игорь Станиславович – канд. мед. наук, доцент

ул. Горького, 80, 230009, г. Гродно

Igor S. Dovnar – Ph. D. (Med.), Associate Professor

80, Gorky Str., 230009, Grodno 

dovigor16@gmail.comИоскевичН. Н.IaskevichN. N.

Иоскевич Николай Николаевич – д-р мед. наук, профессор

ул. Горького, 80, 230009, г. Гродно

Nikolai N. Iaskevich – D. Sc. (Med.), Professor

80, Gorky Str., 230009, Grodno 

inngrno@mail.ruГродненский государственный медицинский университетGrodno State Medical UniversityИнститут элементоорганических соединений имени А. Н. Несмеянова РАНA. N. Nesmeyanov Institute of Organoelement Compounds of the Russian Academy of Sciences202106092021183351361Copyright © Довнар Р.И., Васильков А.Ю., Соколова Т.Н., Наумкин А.В., Будников А.В., Довнар И.С., Иоскевич Н.Н., 20212021Довнар Р.И., Васильков А.Ю., Соколова Т.Н., Наумкин А.В., Будников А.В., Довнар И.С., Иоскевич Н.Н.Dovnar R.I., Vasil’kov A.Y., Sakalova T.M., Naumkin A.V., Budnikov A.V., Dovnar I.S., Iaskevich N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/786

Проведена количественная оценка антибактериального влияния наночастиц серебра на полиантибиотикорезистентные грамположительные и грамотрицательные микроорганизмы. Наночастицы серебра синтезированы экологически допустимым методом металло-парового синтеза. Размер и электронное состояние наночастиц исследованы методами просвечивающей электронной и рентгеновской фотоэлектронной спектроскопии. Оценка антибактериальных свойств наноматериалов выполнена на двух клинических патогенных штаммах грамположительных и четырех штаммах грамотрицательных микроорганизмов. Типирование и оценка устойчивости бактерий к антибиотикам проведены на микробиологическом анализаторе. Количественная оценка антибактериального влияния наночастиц выполнена с использованием метода разведений и путем определения минимальных ингибирующей и бактерицидной концентраций.Установлено, что размер изучаемых наночастиц серебра составляет от 5 до 24 нм, средний диаметр ‒ 10,8 нм. Показано, что для всех использованных в исследовании клинических штаммов микроорганизмов характерна множественная антибактериальная устойчивость (процент антибиотикорезистентности для них составляет от 12,5 до 93,3 %). Установлено, что для исследованных микроорганизмов значения минимальной ингибирующей концентрации (МИК) находятся в интервале от 7,81 до 31,25 мкг/мл, а минимальной бактерицидной концентрации (МБК) – в пределах от 31,25 до 62,50 мкг/мл.Полученные по МИК и МБК данные могут быть использованы при создании перспективных антимикробных препаратов и изделий медицинского назначения следующего поколения.

A quantitative assessment of the antibacterial effect of silver nanoparticles on polyantibiotic-resistant grampositive and gram-negative microorganisms was carried out. Silver nanoparticles were synthesized by the environmentally friendly metal-steam synthesis method. The size and electronic state of nanoparticles were investigated by transmission electron and X-ray photoelectron spectroscopy. The antibacterial properties of nanomaterials were assessed on two clinical pathogenic strains of gram-positive and four strains of gram-negative microorganisms. The typing and assessment of bacterial resistance to antibiotics were carried out on a microbiological analyzer. The antibacterial effect of nanoparticles was quantitatively assessed using the dilution method and the determination of the minimum inhibitory and minimum bactericidal concentrations.It was found that the studied silver nanoparticles have sizes in the range from 5 to 24 nm with an average diameter of 10.8 nm. It was shown that all clinical strains of microorganisms used in the study are characterized by multiple antibacterial resistance; the percentage of their antibiotic resistance ranges from 12.5 to 93.3 %. It was found that for the studied microorganism, the values of the minimum inhibitory concentration (MIC) are in the range from 7.81 to 31.25 μg/ml, and the minimum bactericidal concentration (MBC) is in the range from 31.25 to 62.50 μg/ml. The obtained MIC and MBC data can be used to create promising antimicrobial drugs and medical next generation devices.

наночастицы серебраантибиотикирезистентностьмикроорганизмыминимальная ингибирующая концентрацияминимальная бактерицидная концентрацияsilver nanoparticlesantibioticsresistancemicroorganismsminimum inhibitory concentrationminimum bactericidal concentrationРабота выполнена при финансовой поддержке Белорусского республиканского фонда фундаментальных исследований (проект M20P-086) и Российского фонда фундаментальных исследований (грант № 20-53-00030 Бел-a). Исследования методом ПЭМ и РФЭС проведены при поддержке Министерства науки и высшего образования Российской Федерации с использованием научного оборудования Центра исследования строения молекул Института элементоорганических соединений имени А. Н. Несмеянова РАН.This work was financially supported by the Belarusian Republican Foundation for Basic Research (project M20P-086) and RFBR (project No. 20-53-00030). 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The authors declare that there are no conflicts of interest present.