vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2021-18-2-204-211vestim-762Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEИсследование действия некоторых препаратов, применяемых в клинике, на активность AQP1 мембраны эритроцитов человекаStudy of the effect of some drugs used in the clinic on the AQP1 activity of the human erythrocyte membraneБосяковаЕ. В.BasiakovaK. V.

Босякова Екатерина Владимировна – науч. сотрудник

ул. Ф. Скорины, 24, 220114, г. Минск

Katerina V. Basiakova – Researcher

24, F. Skorina Str., 220114, Minsk 

basiakova.k.v@gmail.comТитовецЭ. П.TitovetsE. P.

Титовец Эрнст Петрович – д-р биол. наук, профессор, гл. науч. сотрудник

ул. Ф. Скорины, 24, 220114, г. Минск 

Ernst P. Titovets – D. Sc. (Biol.), Professor, Chief Researcher

24, F. Skorina Str., 220114, Minsk 

eptitovets@gmail.comРеспубликанский научно-практический центр неврологии и нейрохирургииRepublican Research and Practical Center of Neurology and Neurosurgery202104062021182204211Copyright © Босякова Е.В., Титовец Э.П., 20212021Босякова Е.В., Титовец Э.П.Basiakova K.V., Titovets E.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/762

С использованием метода остановленного потока и на основе исследования интенсивности светорассеяния изучено действие применяемых в клинике фармакологических препаратов на водный обмен эритроцитов человека, катализируемый аквапорином AQP1. Установлено, что примененные в терапевтических концентрациях лекарственные средства оказывают разной степени выраженности ингибирующее влияние на водную проницаемость мембраны эритроцитов. Полученные результаты расширяют представление о молекулярном механизме действия исследованных нами препаратов. С учетом широкого распространения AQP1 в различных тканях человека эти данные следует принимать во внимание при проведении терапевтических мероприятий, направленных на нормализацию водного обмена органов и тканей.

Using the stopped flow method and based on the study of the intensity of light scattering, the effect of pharmacological preparations used in the clinic on the water exchange of human erythrocytes, catalyzed by aquaporin AQP1, was studied. Pharmacological preparations used in therapeutic concentrations have a variable inhibitory effect on water permeability of the erythrocyte membrane. The obtained results broaden our understanding of the molecular action mechanism of the investigated drugs. In view of the wide distribution of AQP1 in various human tissues, these data should be taken into account when carrying out therapeutic measures aimed at normalizing the water exchange of organs and tissues.

эритроцитыAQP1фармакологические препаратыводный обмен тканейкинетика набухания эритроцитовred blood cellsAQP1tissue water metabolismpharmacological preparationskinetics of erythrocyte swellingReferencesTakata, K. Aquaporins: water channel proteins of the cell membrane / K. Takata, T. Matsuzaki, Y. Tajika // Prog. Histochem. Cytochem. – 2004. – Vol. 39, N 1. – P. 1–83. https://doi.org/10.1016/j.proghi.2004.03.001Takata K., Matsuzaki T., Tajika Y. Aquaporins: water channel proteins of the cell membrane. Progress in Histochemistry and Cytochemistry, 2004, vol. 39, no. 1, pp. 1–83. https://doi.org/10.1016/j.proghi.2004.03.001Titovets, E. Novel computational model of the brain water metabolism: introducing an interdisciplinary approach / E. Titovets // J. Comp. Biol. Sys. – 2018. – Vol. 2, N 1. – Art. 103.Titovets E. Novel computational model of the brain water metabolism: introducing an interdisciplinary approach. Journal of Computational Systems Biology, 2018, vol. 2, no. 1, art. 103.Titovets, E. Nanofluidic approach to brain water metabolism / E. Titovets // Adv. Nanomed. Nanotechnol. Res. – 2019. – Vol. 1. – P. 49–56.Titovets E. Nanofluidic approach to brain water metabolism. Advances in Nanomedicine and Nanotechnology Research, 2019, vol. 1, pp. 49–56.The brain interstitial system: Anatomy, modeling, in vivo measurement, and applications / Y. Lei [et al.] // Prog. Neurobiol. – 2017. – Vol. 157. – P. 230–246. https://doi.org/10.1016/j.pneurobio.2015.12.007Lei Y., Han H., Yuan F., Javeed A., Zhao Y. The brain interstitial system: anatomy, modeling, in vivo measurement, and applications. Progress in Neurobiology, 2017, vol. 157, pp. 230–246. https://doi.org/10.1016/j.pneurobio.2015.12.007Simon, M. J. Regulation of cerebrospinal fluid (CSF) flow in neurodegenerative, neurovascular and neuroinflammatory disease / M. J. Simon, J. J. Iliff // Biochim. Biophys. Acta. Mol. Basis. Dis. – 2016. – Vol. 1862, N 3. – P. 442–451. https://doi.org/10.1016/j.bbadis.2015.10.014Simon M. J., Iliff J. J. Regulation of cerebrospinal fluid (CSF) flow in neurodegenerative, neurovascular and neuroinflammatory disease. Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease, 2016, vol. 1862, no. 3, pp. 442–451. https://doi.org/10.1016/j.bbadis.2015.10.014Extrarenal tissue distribution of CHIP28 water channels by in situ hybridization and antibody staining / H. Hasegawa [et al.] // Am. J. Physiol. Cell Physiol. – 1994. – Vol. 266, N 4. – P. C893–C903. https://doi.org/10.1152/ajpcell.1994.266.4.c893Hasegawa H., Lian S. C., Finkbeiner W. E., Verkman A. S. Extrarenal tissue distribution of CHIP28 water channels by in situ hybridization and antibody staining. American Journal of Physiology – Cell Physiology, 1994, vol. 266, no. 4, pp. C893–C903. https://doi.org/10.1152/ajpcell.1994.266.4.c893Титовец, Э. П. Аквапорины человека и животных. Фундаментальные и клинические аспекты / Э. П. Титовец. – Минск : Белорус. наука, 2007. – 239 с.Titovets E. P. Aquaporins of man and animals: fundamental and clinical aspects. Minsk, Belorusskaya Nauka Publ., 2007. 239 p. (in Russian).Yool, A. J. Aquaporins: multiple roles in the central nervous system / A. J. Yool // Neuroscientist. – 2007. – Vol. 13, N 5. – P. 470–485. https://doi.org/10.1177/1073858407303081Yool A. J. Aquaporins: multiple roles in the central nervous system. Neuroscientist, 2007, vol. 13, no. 5, pp. 470–485. https://doi.org/10.1177/1073858407303081Yang, B. Erythrocyte water permeability and renal function in double knockout mice lacking aquaporin-1 and aquaporin-3 / B. Yang, T. Ma, A. S. Verkman // J. Biol. Chem. – 2001. – Vol. 276, N 1. – P. 624–628. https://doi.org/10.1074/jbc.m008664200Yang B., Ma T., Verkman A. S. Erythrocyte water permeability and renal function in double knockout mice lacking aquaporin-1 and aquaporin-3. Journal of Biological Chemistry, 2001, vol. 276, no. 1, pp. 624–628. https://doi.org/10.1074/jbc. m008664200Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules / B. M. Denker [et al.] // J. Biol. Chem. – 1988. – Vol. 263, N 30. – P. 15634–15642. https://doi.org/10.1016/s0021-9258(19)37635-5Denker B. M., Smith B. L., Kuhajda F. P., Agre P. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. Journal of Biological Chemistry, 1988, vol. 263, no. 30, pp. 15634–15642. https://doi.org/10.1016/s0021-9258(19)37635-5Higaa, K. Molecular cloning and expression of aquapolin 1 (AQP1) in dog kidney and erythroblasts / K. Higaa, H. Ochiai, H. Fujise // Biochimica et Biophysica Acta (BBA) – Biomembranes. – 2000. – Vol. 146, N 2. – P. 374–382. https://doi.org/10.1016/s0005-2736(99)00218-7Higaa K., Ochiai H., Fujise H. Molecular cloning and expression of aquapolin 1 (AQP1) in dog kidney and erythroblasts. Biochimica et Biophysica Acta (BBA) – Biomembranes, 2000, vol. 1463, no. 2, pp. 374–382. https://doi.org/10.1016/s0005-2736(99)00218-7Wintour, E. M. Water channels and urea transporters // E. M. Wintour // Clin. Exp. Pharmacol. Physiol. – 1997. – Vol. 24, N 1. – P. 1–9. https://doi.org/10.1111/j.1440-1681.1997.tb01775.xWintour E. M. Water channels and urea transporters. Clinical and Experimental Pharmacology and Physiology, 1997, vol. 24, no. 1, pp. 1–9. https://doi.org/10.1111/j.1440-1681.1997.tb01775.xAbbott, N. J. Astrocyte-endothelial interactions at the blood-brain barrier / N. J. Abbott, L. Rönnbäck, E. Hansson // Nat. Rev. Neurosci. – 2006. – Vol. 7, N 1. – P. 41–53. https://doi.org/10.1038/nrn1824Abbott N. J., Rönnbäck L., Hansson E. Astrocyte-endothelial interactions at the blood-brain barrier. Nature Reviews Neuroscience, 2006, vol. 7, no. 1, pp. 41–53. https://doi.org/10.1038/nrn1824Титовец, Э. П. Церебральный отек и современные направления его лечения / Э. П. Титовец, А. Ф. Смеянович // Вес. Нац. акад. навук Беларусі. Сер. мед. навук. – 2011. – № 1. – С. 84–94.Titovets E. P., Smeyanovich A. F. Cerebral edema and the latest trends in the therapy. Vestsі Natsyyanal’nai akademіі navuk Belarusі. Seryya medytsynskіkh navuk = Proceedings of the National Academy of Sciences of Belarus. Medical series, 2011, no. 1, pp. 84–94 (in Russian).Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation / E. Steiner [et al.] // Glia. – 2012. – Vol. 60, N 11. – P. 1646–1659. https://doi.org/10.1002/glia.22383Steiner E., Enzmann G. U., Lin S., Ghavampour S., Hannocks M.-J., Zuber B., Rüegg M. A., Sorokin L., Engelhardt B. Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation. Glia, 2012, vol. 60, no. 11, pp. 1646–1669. https://doi.org/10.1002/glia.22383Пархач, Л. П. Новый концептуальный подход к рассмотрению водного обмена головного мозга, церебрального отека, гипоксии и ишемии / Л. П. Пархач, Э. П. Титовец // Молекулярные, мембранные и клеточные основы функционирования биосистем : Междунар. науч. конф. ; Одиннадцатый съезд Белорус. обществ. об-ния фотобиологов и биофизиков, 17–20 июня 2014 г., Минск : сб. ст. : в 2 ч. / редкол. : И. Д. Волотовский [и др.]. – Минск, 2014. – Ч. 1. – С. 339–341.Titovets E. Р., Parkhach L. P. Novel computational model of the brain water metabolism: introducing an interdisciplinary approach. Molekulyarnye, membrannye i kletochnye osnovy funktsionirovaniya biosistem: Mezhdunarodnaya nauchnaya konferentsiya; Odinnadtsatyi s’’ezd Belorusskogo obshchestvennogo ob’’edineniya fotobiologov i biofizikov (17–20 iyunya 2014 goda, Minsk): sbornik statei. Chast’ 1 [Molecular, membrane and cellular bases of the functioning of biosystems: International scientific conference; Eleventh congress of the Belarusian public association of photobiologists and biophysicists (June 17–20, 2014, Minsk) : collection of articles. Pt. 1]. Мinsk, 2014, pp. 339–341 (in Russian).Soveral, G. Aquaporin modulators: a patent review (2010–2015) / G. Soveral, A. Casini // Expert Opin. Ther. Pat. – 2017. – Vol. 27, N 1. – P. 49–62. https://doi.org/10.1080/13543776.2017.1236085Soveral G., Casini A. Aquaporin modulators: a patent review (2010–2015). Expert Opinion on Therapeutic Patents, 2017, vol. 27, no. 1, pp. 49–62. https://doi.org/10.1080/13543776.2017.1236085Ducza, E. Aquaporins during pregnancy: their function and significance / E. Ducza, A. Csányi, R. Gáspár // Int. J. Mol. Sci. – 2017. – Vol. 18, N 12. – P. 2593. https://doi.org/10.3390/ijms18122593Ducza E., Csányi A., Gáspár R. Aquaporins during pregnancy: their function and significance. International Journal of Molecular Sciences, 2017, vol. 18, no. 12, p. 2593. https://doi.org/10.3390/ijms18122593The Colton blood group locus. A linkage analysis / K. H. Zelinski [et al.] // Transfusion. – 1988. – Vol. 28, N 5. – P. 435–438. https://doi.org/10.1046/j.1537-2995.1988.28588337331.xZelinski T., Kaita H., Lewis M., Coghlan G., Philipps S., Belcher E., McAlpine P. J., Coopland G., Wong P. The Colton blood group locus. A linkage analysis. Transfusion, 1988, vol. 28, no. 5, pp. 435–438. https://doi.org/10.1046/j.1537-2995.1988.28588337331.xCorticosteroids induce expression of aquaporin-1 and increase transcellular water transport in rat peritoneum / M.S.Stoenoiu[etal.]//J.Am.Soc.Nephrol.–2003.–Vol.14,N3. –P.555–565.https://doi.org/10.1097/01.asn.0000053420.37216.9eStoenoiu M. S., Ni J., Verkaeren C., Debaix H., Jonas J.-C., Lameire N., Verbavatz J.-M., Devuyst O. Corticosteroids induce expression of aquaporin-1 and increase transcellular water transport in rat peritoneum. Journal of the American Society of Nephrology, 2003, vol. 14, no. 3, pp. 555–565. https://doi.org/10.1097/01.asn.0000053420.37216.9eИсследование механизмов кислородного обмена эритроцитов человека / Э. П. Титовец [и др.] // Medline.ru. Рос. биомед. журн. – 2009. – Т. 10. – С. 425–441.Parkhach L. P., Titovets E. Р, Stepanova T. S., Matusevich L. I. Study of the mechanisms of oxygen metabolism of human erythrocytes. Medline.ru. Rossiiskii biomeditsinskii zhurnal [Medline.ru. Russian biomedical journal], 2009, vol. 10, pp. 425–441 (in Russian).Dexmedetomidine preconditioning attenuates global cerebral ischemic injury following asphyxial cardiac arrest / X.-D. Ding [et al.] // Int. J. Neurosci. – 2016. – Vol. 126, N 3. – P. 249–256. https://doi.org/10.3109/00207454.2015.1005291Ding X.-D., Zheng N.-N., Cao Y.-Y., Zhao G.-Y., Zhao P. Dexmedetomidine preconditioning attenuates global cerebral ischemic injury following asphyxial cardiac arrest. International Journal of Neuroscience, 2016, vol. 126, no. 3, pp. 249–256. https://doi.org/10.3109/00207454.2015.1005291AQP4 expression in striatal primary cultures is regulated by dopamine – implications for proliferation of astrocytes / E. Küppers [et al.] // Eur. J. Neurosci. – 2008. – Vol. 28, N 11. – P. 2173–2182. https://doi.org/10.1111/j.1460-9568.2008.06531.xKüppers E., Gleiser C., Brito V., Wachter B., Pauly T., Hirt B., Grissmer S. AQP4 expression in striatal primary cultures is regulated by dopamine – implications for proliferation of astrocytes. European Journal of Neuroscience, 2008, vol. 28, no. 11, pp. 2173–2182. https://doi.org/10.1111/j.1460-9568.2008.06531.xAqF026 is a pharmacologic agonist of the water channel aquaporin-1 / A. J. Yool [et al]. // J. Am. Soc. Nephrol. – 2013. – Vol. 24, N 7. – P. 1045–1052. https://doi.org/10.1681/asn.2012080869Yool A. J., Morelle J., Cnops Y., Verbavatz J.-M., Campbell M. E., Beckett E. A., Booker G. W., Flynn G., Devuyst O. AqF026 is a pharmacologic agonist of the water channel aquaporin-1. Journal of the American Society of Nephrology, 2013, vol. 24, no. 7, pp. 1045–1052. https://doi.org/10.1681/asn.2012080869Voigtlaender, J. Transmembrane water influx via aquaporin-1 is inhibited by barbiturates and propofol in red blood cells / J. Voigtlaender, B. Heindl, F. B. Becker // Naunyn-Schmiedeberg’s Arch. Pharmacol. – 2002. – Vol. 366, N 3. – P. 209–217. https://doi.org/10.1007/s00210-002-0580-8Voigtlaender J., Heindl B., Becker F. B. Transmembrane water influx via aquaporin-1 is inhibited by barbiturates and propofol in red blood cells. Naunyn-Schmiedeberg’s Archives of Pharmacology, 2002, vol. 366, no. 3, pp. 209–217. https://doi.org/10.1007/s00210-002-0580-8Propofol administration modulates AQP-4 expression and brain edema after traumatic brain injury / Z. Ding [et al.] // Cell Biochem. Biophys. – 2013. – Vol. 67, N 2. – P. 615–622. https://doi.org/10.1007/s12013-013-9549-0Ding Z., Jiaming Z., Jinyu X., Guangjie S., Guorong H. Propofol administration modulates AQP-4 expression and brain edema after traumatic brain injury. Cell Biochemistry and Biophysics, 2013, vol. 67, no. 2, pp. 615–622. https://doi.org/10.1007/s12013-013-9549-0Propofol pretreatment attenuates aquaporin-4 over-expression and alleviates cerebral edema after transient focal brain ischemia reperfusion in rats / Y. Y. Zheng [et al.] // Anesth. Analg. – 2008. – Vol. 107, N 6. – P. 2009–2016. https://doi.org/10.1213/ane.0b013e318187c313Yue-Ying Z., Yun-Ping L., Hui-Fang T., Sheng-Mei Z. Propofol pretreatment attenuates aquaporin-4 over-expression and alleviates cerebral edema after transient focal brain ischemia reperfusion in rats. Anesthesia and Analgesia, 2008, vol. 107, no. 6, pp. 2009–2016. https://doi.org/10.1213/ane.0b013e318187c313Sevoflurane modulates AQPs (1,5) expression and endoplasmic reticulum stress in mice lung with allergic airway inflammation / C. M. Lv [et al.] // Biosci. Rep. – 2019. – Vol. 39, N 11. – Art. BSR20192453. https://doi.org/10.1042/bsr20193282Chang-Ming Lv., Hui-Mei W., Ling W., Guang-Hong X., Zhi-Lai Y., Qi-Ying S. Sevoflurane modulates AQPs (1,5) expression and endoplasmic reticulum stress in mice lung with allergic airway inflammation. Bioscience Reports, 2019, vol. 39, no. 11, art. BSR20192453. https://doi.org/10.1042/bsr20193282Kamondi, A. Mechanisms of antihistamine-induced sedation in the human brain: H1 receptor activation reduces a background leakage potassium current / A. Kamondi, P. B. Reiner // Neuroscience. – 1994. – Vol. 59, N 3. – P. 579–588. ttps://doi.org/10.1016/0306-4522(94)90178-3Kamondi A., Reiner P. B. Mechanisms of antihistamine-induced sedation in the human brain: H1 receptor activation reduces a background leakage potassium current. Neuroscience, 1994, vol. 59, no. 3, pp. 579–588. https://doi.org/10.1016/0306-4522(94)90178-3Антиоксидантная терапия при лечении перитуморального отека головного мозга // А. Ф. Смеянович [и др.] // Достижения медицинской науки Беларуси : рец. науч.-практ. ежег. / гл. ред. И. Н. Семененя. – Минск, 2008. – Вып. 13. – С. 92–93.Smeyanovich A. F., Titovets E. P., Lukasheiko Yu. N., Parkhach L. P., Shkut D. N., Bulgak V. V. Antioxidant therapy in treating brain peritumor edema. Dostizheniya meditsinskoi nauki Belarusi: retsenziruemyi nauchno-prakticheskii ezhegodnik [Accomplishments of medical science in Belarus: peer-reviewed scientific and practical yearbook]. Мinsk, 2008, iss. 13, pp. 92–93 (in Russian).The effect of ketorolac and triamcinolone acetonide on adipogenic and hepatogenic differentiation through miRNAs 16/15/195: Possible clinical application in regenerative medicine / S. F. Aval [et al.] // Biomed. Pharmacother. – 2018. – Vol. 97. – P. 675–683. https://doi.org/10.1016/j.biopha.2017.10.126Aval S. F., Zarghami N., Alizadeh E., Mohammadi S. A. The effect of ketorolac and triamcinolone acetonide on adipogenic and hepatogenic differentiation through miRNAs 16/15/195: Possible clinical application in regenerative medicine. Biomedicine and Pharmacotherapy, 2018, vol. 97, pp. 675–683. https://doi.org/10.1016/j.biopha.2017.10.126Moon, C. Aqp1 expression in erythroleukemia cells: genetic regulation of glucocorticoid and chemical induction / C. Moon, L. S. King, P. Agre // Am. J. Physiol. Cell Physiol. – 1997. – Vol. 273, N 5. – P. C1562–C1570. https://doi.org/10.1152/ajpcell.1997.273.5.c1562Moon C., King L. S., Agre P. Aqp1 expression in erythroleukemia cells: geneticregulation of glucocorticoid and chemical induction. American Journal of Physiology – Cell Physiology, 1997, vol. 273, no. 5, pp. C1562–C1570. https://doi.org/10.1152/ajpcell.1997.273.5.c1562Batista de Almeida, A. F. Aquaporins in health and disease / A. F. Batista de Almeida // Metallodrugs as protein modulators : Ph. D. Thesis / A. F. Batista de Almeida ; University of Groningen. – Groningen, 2016. – P. 59–63.Batista de Almeida A. F. Aquaporins in health and disease. Metallodrugs as protein modulators. Ph.D. Thesis. Groningen, 2016, pp. 59–63.Gold coordination compounds as chemical probes to unravel aquaporin-7 function / A. Madeira [et al.] // Chem. Bio. Chem. – 2014. – Vol. 15, N 10. – P. 1487–1494. ttps://doi.org/10.1002/cbic.201402103Madeira A., de Almeida A., de Graaf C., Camps M., Zorzano A., Moura T. F., Casini A., Soveral G. Gold coordination compounds as chemical probes to unravel aquaporin-7 function. ChemBioChem, 2014, vol. 15, no. 10, pp. 1487–1494. ttps://doi.org/10.1002/cbic.201402103Evidence that aquaporin 1 is a major pathway for CO2 transport across the human erythrocyte membrane / V. Endeward [et al.] // FASEB J. – 2006. – Vol. 20, N 12. – P. 1974–1981. https://doi.org/10.1096/fj.04-3300comEndeward V., Musa-Aziz R., Cooper G. J., Chen L.-M., Pelletier M. F., Virkki L. V., Supuran C. T., King L. S., Boron W. F., Gros G. Evidence that aquaporin 1 is a major pathway for CO2 transport across the human erythrocyte membrane. FASEB Journal, 2006, vol. 20, no. 12, pp. 1974–1981. https://doi.org/10.1096/fj.04-3300comBoron, W. F. Sharpey-Schafer lecture: gas channels / W. F. Boron // Exp. Physiol. – 2010. – Vol. 95, N 12. https://doi.org/10.1113/expphysiol.2010.055244Boron W. F. Sharpey-Schafer lecture: gas channels. Experimental Physiology, 2010, vol. 95, no. 12, pp. 1107–1130. https://doi.org/10.1113/expphysiol.2010.055244Verkman, A. S. Does aquaporin-1 pass gas? An opposing view / A. S. Verkman // J. Physiol. – 2002. – Vol. 542, N 1. – P. 31. https://doi.org/10.1113/jphysiol.2002.024398Verkman A. S. Does aquaporin-1 pass gas? An opposing view. Journal of Physiology, 2002, vol. 542, no. 1, p. 31. https://doi.org/10.1113/jphysiol.2002.024398

The authors declare that there are no conflicts of interest present.