vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2021-18-2-196-203vestim-761Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEОсобенности экспресс-диагностики в периодонтологии (на англ. яз.)Specific characteristics of rapid diagnosis in periodontologyРубниковичС. П.RubnikovichS. P.

Рубникович Сергей Петрович – д-р мед. наук, профессор, ректор

пр. Дзержинского, 83, 220116, г. Минск

Sergey P. Rubnikovich – D. Sc. (Med.), Professor, Rector

83, Dzerzhinski Ave., 220116, Minsk 

rubnikovich@mail.ruДедоваЛ. Н.DedovaL. N.

Дедова Людмила Николаевна – д-р мед. наук, профессор

пр. Дзержинского, 83, 220116, г. Минск

Liudmila N. Dedova ‒ D. Sc. (Med.), Professor

83, Dzerzhinski Ave., 220116, Minsk 

Dedova.bsmu@mail.ruСемижонП. А.SemizhonP. A.

Семижон Павел Анатольевич – канд. биол. наук

ул. Филимонова, 23, 220114, г. Минск

Pavel A. Semizhon ‒ Ph. D. (Biol.)

23, Filimonov Str., 220114, Minsk 

pavel5555@tut.byДенисоваЮ. Л.DenisovaYu. L.

Денисова Юлия Леонидовна – д-р мед. наук, профессор

пр. Дзержинского, 83, 220116, г. Минск

Yuliya L. Denisova – D. Sc. (Med.), Professor

83, Dzerzhinski Ave., 220116, Minsk 

denisova-yul@mail.ruКандрукевичО. В.KandrukevichO. V.

Кандрукевич Ольга Викторовна – канд. мед. наук, доцент

пр. Дзержинского, 83, 220116, г. Минск

Olga V. Kandrukevich ‒ Ph. D. (Med.), Associate Professor

83, Dzerzhinski Ave., 220116, Minsk 

kondruk@mail.ruБелорусский государственный медицинский университетBelarusian State Medical UniversityРеспубликанский научно-практический центр эпидемиологии и микробиологииRepublican Research and Practical Center for Epidemiology and Microbiology202104062021182196203Copyright © Рубникович С.П., Дедова Л.Н., Семижон П.А., Денисова Ю.Л., Кандрукевич О.В., 20212021Рубникович С.П., Дедова Л.Н., Семижон П.А., Денисова Ю.Л., Кандрукевич О.В.Rubnikovich S.P., Dedova L.N., Semizhon P.A., Denisova Y.L., Kandrukevich O.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/761

В настоящее время в Республике Беларусь перспективным направлением в периодонтологии являются разработка, производство и внедрение отечественного экспресс-теста, который даст возможность врачу широко использовать методы микробиологической диагностики в стоматологической практике.Цель исследования ‒ разработать отечественный экспресс-тест в периодонтологии и обозначить порядок подготовительных клинико-лабораторных мероприятий, используя ПЦР и трипсиноподобную активность периопатогенной микрофлоры (Treponema denticola, Porphyromonas gingivalis, Bacteroides forsythus).С использованием методов клинического обследования тканей периодонта проведено стоматологическое обследование 60 пациентов в возрасте 35–44 лет с клиническими признаками генерализованного периодонтита и глубиной патологического зубодесневого кармана 5–6 мм (основная группа) и 140 пациентов в возрасте 20–24 года без признаков болезней периодонта (контрольная группа), обратившихся на 3-ю кафедру терапевтической стоматологии БГМУ. Лабораторная диагностика заключалась в оценке трипсиноподобной активности периопатогенной микрофлоры с помощью ПЦР. Предложен алгоритм подготовительных клинико-лабораторных мероприятий в диагностике болезней периодонта, включающий забор материала и выявление в образце периопатогенных микроорганизмов с использованием биохимического метода; забор материала, его консервация и хранение для исследования методом количественной ПЦР; выявление генетического материала периопатогенных микроорганизмов методом количественной ПЦР в режиме реального времени.Основные принципы микробиологической диагностики в периодонтологии дают возможность достоверно поставить диагноз, планировать лечение и оценивать его результаты.Изучено содержимое десневой борозды и/или периодонтального кармана у пациентов с болезнями периодонта. Для извлечения содержимого из периодонтального кармана у пациентов с болезнями периодонта наиболее рациональным является использование бумажного штифта как стоматологического инструмента.При разработке отечественного экспресс-теста в периодонтологии целесообразным представляется проведение подготовительных клинико-лабораторных мероприятий с использованием ПЦР и оценка трипсиноподобной активности периопатогенной микрофлоры (Treponema denticola, Porphyromonas gingivalis, Bacteroides forsythus).

Currently, the advanced studies have been justified and implemented in the Republic of Belarus that enable the production and a practical use of a domestic rapid test and allow dentists to apply microbiological diagnosis methods.The objective of the study was to develop a domestic rapid test for periodontology, indicating the procedure for preparatory clinical and laboratory measures using PCR and trypsin-like activity of periopathogenic microflora (Treponema denticola, Porphyromonas gingivalis, Bacteroides forsythus)Dental examination was performed in 60 patients with clinical signs of generalized periodontitis and a 5‒6 mm deep pathological dentogingival pocket (PDGP), aged 35–44 (the main group), and 140 patients without signs of periodontal diseases, aged 20–24 (the control group). These patients were selected among the visitors of Chair No. 3 of the Belarusian State Medical University. The laboratory diagnosis was made using PCR and evaluating the trypsin-like activity of periopathogenic microflora.The algorithm of preparatory clinical and laboratory measures was suggested for diagnosis of periodontal diseases, including material sampling and identification of periopathogenic microorganisms in the sample using a biochemical method; sampling, preservation and storage of material for quantitative PRC testing; and the identification of the genetic material of periopathogenic microorganisms by means of a real-time quantitative PRC method.The basic principles of microbiological diagnosis in periodontology enable making a true diagnosis, planning treatment and assessing the treatment results.We examined the content of the gingival sulcus and/or the periodontal pocket in patients who have periodontal diseases. It was noted that the most rational tools for removing the periodontal pocket content in patients with periodontal diseases was a paper pin.The development of a domestic express test in periodontology demonstrated the need of preparatory clinical and laboratory activities using PCR and trypsin-like activity of periopathogenic microflora (Treponema denticola, Porphyromonas gingivalis, Bacteroides forsythus).

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The authors declare that there are no conflicts of interest present.