vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2021-18-1-36-45vestim-731Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEХондрогенный и остеогенный потенциал мезенхимальных стволовых клеток костного мозга и плацентыOsteogenic and chondrogenic differentiation potential of mesenchymal stem cells obtained from the bone marrow and placentaЖерносеченкоА. А.ZhernasechankaH. A.

Жерносеченко Анна Александровна – науч. сотрудник

ул. Фрунзенская, 43, 223053, д. Боровляны, Минский р-н, Республика Беларусь

Hanna A. Zhernasechanka – Researcher

43, Frunzenskaya Str., 223053, v. Borovliany, Minsk Region, Republic of Belarus

sapphire.anna@gmail.comИсайкинаЯ. И.IsaikinaYa. I.

Исайкина Янина Ивановна – канд. биол. наук, заведующий лабораторией

ул. Фрунзенская, 43, 223053, д. Боровляны, Минский р-н, Республика Беларусь

Yanina I. Isaikina ‒ Ph. D. (Biol.), Head of the Laboratory

43, Frunzenskaya Str., 223053, v. Borovliany, Minsk Region, Republic of Belarus

yaninai@mail.ruФилиповичТ. В.FilipovichT. V.

Филипович Татьяна Валерьевна ‒ мл. науч. сотрудник

ул. Фрунзенская, 43, 223053, д. Боровляны, Минский р-н, Республика Беларусь

Tatiana V. Filipovich ‒ Junior Researcher

43, Frunzenskaya Str., 223053, v. Borovliany, Minsk Region, Republic of Belarus

tatianafilipovich95@gmail.comЛяхЕ. Г.LiakhE. G.

Лях Елена Геннадьевна ‒ науч. сотрудник

ул. Фрунзенская, 43, 223053, д. Боровляны, Минский р-н, Республика Беларусь

Elena G. Liakh ‒ Researcher

43, Frunzenskaya Str., 223053, v. Borovliany, Minsk Region, Republic of Belarus

lyakchelena@gmail.comРеспубликанский научно-практический центр детской онкологии, гематологии и иммунологииRepublican Research Center for Pediatric Oncology, Hematology and Immunology2021230220211813645Copyright © Жерносеченко А.А., Исайкина Я.И., Филипович Т.В., Лях Е.Г., 20212021Жерносеченко А.А., Исайкина Я.И., Филипович Т.В., Лях Е.Г.Zhernasechanka H.A., Isaikina Y.I., Filipovich T.V., Liakh E.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/731

Мезенхимальные стволовые клетки (МСК) представляют собой перспективный ресурс для клеточных биотехнологий. Тем не менее вопрос о преимуществах использования МСК различного происхождения для хондро- и остеогенной дифференцировки требует дальнейшего изучения.

Целью данного исследования являлось проведение анализа остеохондрогенного дифференцировочного потенциала МСК, полученных из костного мозга и тканей плаценты. Результаты наших исследований свидетельствуют, что МСК как костного мозга, так и плаценты под влиянием индукционных факторов дифференцируются в хондрогенном направлении. Тем не менее реализация программы хондрогенеза в МСК костного мозга происходит преимущественно за счет экспрессии коллагенов (Coll2, Coll10), в то время как для МСК плаценты характерен более высокий синтез неколлагеновых белков внеклеточного матрикса (COMP, Ver). В связи с этим применение МСК костного мозга в инженерии хрящевой ткани более предпочтительно. Оценка синтеза щелочной фосфатазы и накопления кальциевых депозитов в дифференцированных клетках показала, что остеогенный дифференцировочный потенциал МСК костного мозга выше, чем МСК плаценты. МСК костного мозга, находясь в составе фибринового геля, дифференцируются в остеогенном направлении под влиянием индукционных факторов на 14-й день, что подтверждается синтезом щелочной фосфатазы, депонированием солей кальция внутри клетки и во внеклеточном матриксе, увеличением экспрессии мРНК Sp7 и DMP.

Mesenchymal stem cells (MSC) represent a perspective resource for cell biotechnology. However the question of chondrogenic and osteogenic capacity of MSC of different origin remains under study.

The aim of this study was to analyze the osteo-chondrogenic differentiation potential of MSC obtained from the bone marrow and placenta. The results of our studies have indicated that bone marrow-derived and placenta-derived MSC showed a chondrogenic potential in vitro after a chondrogenic induction with specific differentiation media. But for bone marrowderived MSC, the chondrogenic program was realized by expression of collagens (Coll2, Coll10), while in placenta-derived MSC cultures we found a progressive increase in COMP and Ver expression, so bone marrow-derived MSC is more preferable for use in cartilage tissue engineering. Regarding the results on alkaline phosphatase and alizarin red staining, bone marrowderived MSC showed a more significant osteogenic potential compared to placenta-derived MSC. Bone marrow-derived MSC in the composition of fibrin gel after osteogenic induction on the 14th day exhibited the activity of alkaline phosphatase, calcium depositions inside the cells and extracellular matrix, the increase in Sp7 and DMP expression.

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The authors declare that there are no conflicts of interest present.