vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2020-17-4-461-469vestim-715Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEИзменение концентраций серосодержащих аминокислот в головном мозге после метиониновой нагрузки в экспериментеChanges in the concentration of sulfur-containing amino acids in the brain after methionine load in the experimentChanges in the concentration of sulfur-containing amino acids in the brain after methionine load in the experimenthttps://orcid.org/0000-0001-9505-6717НовогродскаяЯ. И.NovogrodskayaYa. I.

Новогродская Яна Иосифовна ‒ аспирант

ул. Горького, 80, 230015, г. Гродно

Yana I. Novogrodskaya ‒ Postgraduate student

80, Gorky Str., 230015, Grodno

yananovogrodskaya@mail.ruДорошенкоЕ. М.DoroshenkoYe. M.

Дорошенко Евгений Михайлович ‒ кандидат биологических наук, доцент, ведущий научный сотрудник

ул. Горького, 80, 230015, г. Гродно

Yevgeny M. Doroshenko ‒ Ph. D. (Biol.), Assistant Professor, Leading Researche

80, Gorky Str., 230015, Grodno

dgi03@mail.ruhttps://orcid.org/0000-0001-9939-8749КурбатМ. Н.KurbatM. N.

Курбат Михаил Николаевич ‒ кандидат медицинских наук, доцент, заведующий лабораторией

ул. Горького, 80, 230015, г. Гродно

Mikhail N. Kurbat ‒ Ph. D. (Med.), Assistant Professor, Head of the Laboratory

80, Gorky Str., 230015, Grodno

vwmisha@mail.ruГродненский государственный медицинский университетGrodno State Medical University202005122020174461469Copyright © Новогродская Я.И., Дорошенко Е.М., Курбат М.Н., 20202020Новогродская Я.И., Дорошенко Е.М., Курбат М.Н.Novogrodskaya Y.I., Doroshenko Y.M., Kurbat M.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/715

Изучено влияние метиониновой нагрузки на состояние пула серосодержащих аминокислот и некоторых метаболически родственных им соединений в различных структурах головного мозга крыс. Данные соединения определяли методом обращенно-фазовой высокоэффективной жидкостной хроматографии. Во всех исследованных регионах мозга нагрузка метионином в суточной дозе 3 г/кг приводила к однонаправленному дисбалансу серосодержащих соединений ‒ повышению концентраций метионина и гипотаурина (наиболее выраженное в стриатуме), цистатионина (наиболее выраженное в больших полушариях). Значимое повышение концентрации таурина наблюдалось лишь в гипоталамусе и стриатуме. Во всех отделах мозга, кроме стриатума, отмечалось снижение уровня серина – предшественника транссульфурирования. В мозжечке, по сравнению с другими регионами мозга, наблюдалось повышение содержания цистеиновой кислоты и снижение уровня цистенсульфиновой, что указывает на то, что синтез таурина осуществляется преимущественно по пути окисления последней.

The effect of methionine overload on the state of the pool of sulfur-containing amino acids and their metabolites was studied in the various brain structures determined by reverse phase high performance liquid chromatography (HPLC). In all regions of the brain studied, methionine led to a unidirectional imbalance of sulfur-containing compounds: there was an increase in the concentrations of methionine, cystathionine and hypotaurine. The most pronounced increase in methionine and hypotaurine levels was observed in the striatum, cystathionine in the hemispheres. A significant increase in taurine concentration was observed only in the hypothalamus and striatum. In other parts of the brain a tendency to increase its level was shown. In all brain regions studied except the striatum, serine levels were decreased. In the cerebellum, in comparison with other regions, an increase in the level of cysteic acid and a decrease in the level of cysteinesulfinic acid were observed, which indicates that taurine synthesis is occurred mainly through the cysteine sulfinate oxidation.

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The authors declare that there are no conflicts of interest present.