vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2020-17-4-444-451vestim-713Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEМорфологические изменения в тканях периодонта лабораторных животных при применении мезенхимальных стволовых клетокMorphological changes in periodontal tissues in laboratory animals using mesenchymal stem cellsРубниковичС. П.RubnikovichS. P.

Рубникович Сергей Петрович – доктор медицинских наук, профессор, ректор

пр. Дзержинского, 83, 220116, г. Минск

Sergey P. Rubnikovich ‒ D. Sc. (Med.), Professor, rector

83, Dzerzhynski Ave., 220116, Minsk

rubnikovich@mail.ruСиракС. В.SirakS. V.

Сирак Сергей Владимирович ‒ доктор медицинских наук, профессор, заведующий отделом

ул. Мира, 310, 355017, г. Ставрополь

Sergey V. Sirak ‒ D. Sc. (Med.), Professor, Head of the Department

310, Mir Str., 355017, Stavropol

sergejsirak@yandex.ruДенисоваЮ. Л.DenisovaYu. L.

Денисова Юлия Леонидовна – доктор медицинских наук, профессор

пр. Дзержинского, 83, 220116, г. Минск

Yuliya L. Denisova ‒ D. Sc. (Med.), Professor

83, Dzerzhynski Ave., 220116, Minsk

denisova-yul@mail.ruАндрееваВ. А.AndreevaV. A.

Андреева Василина Анатольевна ‒ кандидат медицинских наук, доцент

ул. П. Бровки, 3/3, 220013, г. Минск

Vasilina A. Andreeva ‒ Ph. D. (Med.), Assistant Professor

83, Dzerzhinski Ave., 220116, Minsk

vasilinastomatolog@tut.byКузьменкоЕ. В.KuzmenkoE. V.

Кузьменко Елена Викторовна ‒ кандидат медицинских наук, доцент

ул. П. Бровки, 3/3, 220013, г. Минск

Elena V. Kuzmenko ‒ Ph. D. (Med.), Assistant Professor

83, Dzerzhinski Ave., 220116, Minsk

elena-stom@yandex.ruВладимирскаяТ. Э.VladimirskayaT. E.

Владимирская Татьяна Эрнстовна ‒ кандидат биологических наук, ведущий научный сотрудник

ул. П. Бровки, 3/3, 220013, г. Минск

Tatiana E. Vladimirskaya – Ph. D. (Biol.), Leading Researcher

83, Dzerzhinski Ave., 220116, Minsk

tan_2304@inbox.ruХомичИ. С.KhomichI. S.

Хомич Илья Станиславович – кандидат медицинских наук, доцент

ул. П. Бровки, 3/3, 220013, г. Минск

Ilya S. Khomich ‒ Ph. D. (Med.), Assistant Professor

83, Dzerzhinski Ave., 220116, Minsk

ilya.khomich@gmail.comВолотовскийИ. Д.VolotovskyI. D.

Волотовский Игорь Дмитриевич ‒ академик, доктор биологических наук, профессор

ул. Академическая, 27, 220072, г. Минск

Igor D. Volotovsky ‒ Academician, D. Sc. (Biol.), Professor

27, Akademicheskaya Str., 220072, Minsk

volot@biobel.basnet.byБелорусский государственный медицинский университетBelarusian State Medical UniversityСтавропольский государственный медицинский университетStavropol State Medical UniversityБелорусская медицинская академия последипломного образованияBelarusian Medical Academy of Postgraduate EducationИнститут биофизики и клеточной инженерии НАН БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus202005122020174444451Copyright © Рубникович С.П., Сирак С.В., Денисова Ю.Л., Андреева В.А., Кузьменко Е.В., Владимирская Т.Э., Хомич И.С., Волотовский И.Д., 20202020Рубникович С.П., Сирак С.В., Денисова Ю.Л., Андреева В.А., Кузьменко Е.В., Владимирская Т.Э., Хомич И.С., Волотовский И.Д.Rubnikovich S.P., Sirak S.V., Denisova Y.L., Andreeva V.A., Kuzmenko E.V., Vladimirskaya T.E., Khomich I.S., Volotovsky I.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/713

В статье приведены результаты исследования морфологических изменений в тканях периодонта лабораторных животных при использовании мезенхимальных стволовых клеток (МСК). Цель исследования – разработать модель экспериментального периодонтита и установить характер морфологических изменений в тканях периодонта при применении биомедицинского клеточного продукта на основе аллогенных МСК жировой ткани (МСК ЖТ). Применение смеси МСК ЖТ и остеоиндуцированных МСК ЖТ в пропорции 1:1 позволяет сократить сроки регенерации костного дефекта по сравнению c длительностью восстановления костной ткани при применении МСК ЖТ, а также остеоиндуцированных МСК ЖТ, что выражается в заполнении дефекта костной ткани наряду с мышечной фиброретикулярной остеогенной тканью через 1 мес. после оперативного вмешательства. Через 2 мес. в зоне дефекта, заполненного коллагеновой мембраной со смесью МСК ЖТ и остеоиндуцированных МСК ЖТ, выявлены признаки начала формирования костных балок, что свидетельствует о более полноценном остеосинтезе, чем при заживлении под кровяным сгустком.

The article presents the analysis of the morphological changes of the periodontal tissues of laboratory animals using mesenchymal stem cells (MSCs). The goal of the study is to create a model of experimental periodontitis and to identify the characteristics of morphological changes in the periodontal tissues using a biomedical cell product based on the allogeneic adipose tissue of MSCs (AT MSCs). The application of a mixture of AT MSCs and osteoinduced AT MSCs (in the ratio of 1:1) allows reducing the time of bone defect regeneration in comparison to that of bone tissue regeneration when AT MSCs and osteoinduced AT MSCs are used separately, which is expressed in the filling of the bone defect with a fibroreticular osteogenic tissue, as well as with a muscle tissue one month after surgery. In 2 months, in the defect area filled with a collagen membrane with a mixture of AT MSCs and osteoinduced AT MSCs, the initial signs of the formation of trabecula of bones were detected, which is evident of a more comprehensive osteosynthesis process compared to the blood clot use.

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The authors declare that there are no conflicts of interest present.