vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2020-17-1-7-19vestim-655Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEЭкспериментальное обоснование способа аутотрансплантации мезенхимальных стволовых клеток для регенерации костной ткани головки бедраExperimental justification of the method of mesenchymal stem cell autotransplantation for regeneration of the femoral head bone tissueМурзичА. Э.MurzichA. E.

Мурзич Александр Эдуардович – кандидат медицинских наук, заведующий лабораторией

ул. Кижеватова, 60/4, 220024, г. Минск

 

Alyaksandr E. Murzich – Ph. D. (Med.), Head of the Laboratory

60, Bld. 4, Kizhevatov Str., 220024, Minsk

mae77@list.ruПашкевичЛ. А.PashkevichL. A.

Пашкевич Людмила Антоновна – доктор медицинских  наук, заведующий лабораторией

ул. Кижеватова, 60/4, 220024, г. Минск

 

Lyudmila A. Pashkevich – D. Sc. (Med.), Head of the Laboratory

60, Bld. 4, Kizhevatov Str., 220024, Minsk

pashkevichla@mail.ruЖерносеченкоА. А.ZhernasechankaH. A.

Жерносеченко Анна Александровна – научный  сотрудник

ул. Фрунзенская, 43, 223053, Минский р-н, д. Боровляны

Hanna A. Zhernasechanka – Researcher

43, Frunzenskaya Str., v. Borovliany, 223053, Minsk Region

sapphire.anna@gmail.comРеспубликанский научно-практический центр травматологии и ортопедииRepublican Scientific and Practical Centre of Traumatology and OrthopedicsРеспубликанский научно-практический центр детской онкологии, гематологии и иммунологииRepublican Research Center for Pediatric Oncology, Hematology and Immunology202018032020171719Copyright © Мурзич А.Э., Пашкевич Л.А., Жерносеченко А.А., 20202020Мурзич А.Э., Пашкевич Л.А., Жерносеченко А.А.Murzich A.E., Pashkevich L.A., Zhernasechanka H.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/655

Представлены результаты экспериментальной работы по обоснованию нового способа восстановления костной ткани головки бедренной кости с помощью мезенхимальных стволовых клеток (МСК). Проведены оперативные вмешательства на 5 собаках, включающие эксфузию костного мозга, получение МСК и их последующую остеогенную дифференцировку, подбор носителя для МСК, создание дефектов головки бедра, имплантацию клеточного продукта в дефект кости. После операций осуществляли наблюдение и уход за экспериментальными животными, а затем выводили их из эксперимента и производили забор биологического материала. Результаты гистологического исследования микропрепаратов головок бедренных костей собак подтверждают образование зрелой костной ткани компактного строения с остеобластами на месте введения МСК. В экспериментальных образцах контрольной группы (без введения МСК) место дефекта выполнено грубоволокнистой остеобластической тканью без формирования костных структур. Полученный экспериментальный материал позволит разработать метод аутотрансплантации МСК для регенеративного восстановления повреждений костной ткани при остеонекрозе головки бедра у взрослых, который будет внедрен в систему практического здравоохранения.

The results of experimental justification of a new method for restoring the femoral head bone tissue with the use of mesenchymal stem cells (MSCs) are presented. Surgical interventions were performed on 5 dogs, including the bone marrow exfusion, the obtaining of MSCs and their subsequent osteogenic differentiation, the selection of a carrier for MSCs, the creation of femoral head defects, and the implantation of a cellular product into a bone defect. Experimental animals were observed and cared after operations, and removed from the experiment, as well as the biological material was taken. The histological results of the femoral head micropreparations of dogs confirm the formation of the mature bone tissue of compact structure with osteoblasts at the site of MSCs injection. In the control group of the experimental samples (without MSCs), the  The obtained experimental material will allow us to develop a method for autologous transplantation of MSCs for regenerative restoration of bone tissue damage in the adult’s femoral head osteonecrosis, which will find use in the practical healthcare system.

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The authors declare that there are no conflicts of interest present.