vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2019-16-4-410-423vestim-548Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEУплыў кверцэтына і яго камбінацыі з цыкладэкстрынам на загойванне апёкавых ран у лабараторных пацукоўEffect of quercetin and its combination with cyclodextrin on the healing of burn wounds in laboratory ratsБакуновичА. А.BakunovichA. A.

Бакунович Алексей Алексеевич – младший научный сотрудник. 

БЛК, 50, 230030, г. Гродно

Aliaksei A. Bakunovich – Junior researcher. 

50, BLK, 230030, Grodno

baptist.alexei@mail.ruОстровскийА. А.AstrowskiA. A.

Островский Александр Александрович – доктор медицинских наук, профессор, ведущий научный сотрудник. 

БЛК, 50, 230030, г. Гродно

Alexander A. Astrowski – D. Sc. (Med.), Professor, Leading researcher. 

50, BLK, 230030, Grodno

astrowski@gmail.comШляхтунА. Г.ShlyahtunA. H.

Шляхтун Алексей Генрихович – научный сотрудник, заведующий отделом. 

БЛК, 50, 230030, г. Гродно

Alexej H. Shlyahtun – Researcher, Head of the Department. 

50, BLK, 230030, Grodno

shlyahtun@ibiochemistry.byМорозВ. Л.MarozV. L.

Мороз Валентина Леопольдовна – научный сотрудник. 

БЛК, 50, 230030, г. Гродно

Valiantsina L. Maroz – Researcher.

50, BLK, 230030, Grodno

valmor655@gmail.comОстровскаяО. Б.AstrovskajaA. V.

Островская Оксана Борисовна – кандидат биологических наук, старший научный сотрудник. 

ул. Горького, 80, 230009, г. Гродно

Aksana B. Astrovskaja – Ph. D. (Biol.), Senior researcher. 

80, Gorky Str., 230009, Grodno

astrowskaja@gmail.comМеламедВ. Д.MelamedV. D.

Меламед Владимир Дмитриевич – кандидат медицинских наук, доцент. 

ул. Горького, 80, 230009, г. Гродно

Vladimir D. Melamed – Ph. D. (Med.), Assistant Professor. 

80, Gorky Str., 230009, Grodno

alllotransplan@mail.ruБукоВ. У.BukoV. U.

Буко Вячеслав Ульянович – доктор биологических наук, профессор, заведующий отделом. 

БЛК, 50, 230030, г. Гродно

Vyacheslav U. Buko – D. Sc. (Biol.), Professor, Head of the Department. 

50, BLK, 230030, Grodno

buko@bioch.busnet.byИнститут биохимии биологически активных соединений НАН БеларусиInstitute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of BelarusГродненский государственный медицинский университетGrodno State Medical University201905122019164410423Copyright © Бакунович А.А., Островский А.А., Шляхтун А.Г., Мороз В.Л., Островская О.Б., Меламед В.Д., Буко В.У., 20192019Бакунович А.А., Островский А.А., Шляхтун А.Г., Мороз В.Л., Островская О.Б., Меламед В.Д., Буко В.У.Bakunovich A.A., Astrowski A.A., Shlyahtun A.H., Maroz V.L., Astrovskaja A.V., Melamed V.D., Buko V.U.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/548

Изучено заживление ожоговых ран у лабораторных крыс под влиянием кверцетина (КВ) и его комплекса с 2-гидроксипропил-β-циклодекстрином (КВЦД), наносимых на раневую поверхность. Крысам-самцам линии Wistar делали ожоги 3Б степени на участке кожи в межлопаточной области, защищенной камерой, с помощью железного стержня, нагретого до 80 оС. Выполнено две серии опытов: в первой (21 животное) рану старались вести бережно, во второй (18 животных) – в течение 3–7 сут из раны удаляли некротические ткани. В ходе исследования отслеживали динамику заживления, опираясь на морфометрические критерии, выявляли время наступления полной эпидермизации, определяли фагоцитарный индекс нейтрофилов (ФИ) в смывах с раневой поверхности, проводили гистологическое исследование ткани, после декапитации (на 21-е и 43-и сутки) определяли лейкоцитарную формулу крови, ФИ и метаболическую активность нейтрофилов.

Установлено, что удаление некротических тканей с раневой поверхности способствует более быстрому заживлению термического повреждения. Под влиянием данных веществ в первой серии опытов площадь вторичного струпа, присутствующего на раневой поверхности, оказалась меньше на 14-е сутки после повреждения, а в другой – увеличивались темпы эпидермизации раны на заключительном ее этапе. Выявлено позитивное влияние КВ и особенно КВЦД на возвращение к норме лейкоцитарной формулы крови, активизацию фагоцитарной и метаболической активности нейтрофилов на поверхности раны и в крови.

. The aim of this study was to evaluate the effects of quercetin (QC) and its complex with 2-hydroxypropyl-β-cyclodextrin (QECD) in healing burn related skin wounds in the rat model. Male Wistar rats were subjected to third-degree burn injury of skin in the interscapular area using a metal rod heated to 80 °C. The area of the skin which the burn was applied to was secured from the surroundings by the protective chamber. In order to estimate the efficacy of different treatment modalities the experiments were carried out in two stages. On first stage of the study, the group of animals (n = 21) was subjected to burn injury and the wound surface was not affected with additional procedures except the substances for treatment being applied. During the second stage of the study with another group of animals (n = 18) the removal of necrotic tissue was carried out over the period of 3 to 7 days. The progress of the wound healing was followed by performing morphometric analyses in order to determine complete re-epithelialization. The phagocytic index of neutrophils was determined in washouts from the wounds during the healing process. The animals used on the first stage of the study were sacrificed at day 21 of the experiment and those on the second stage at day 43 and the tissues were subjected to histological examination. The amounts of white blood cells and phagocytic index of neutrophils were calculated in blood samples followed by the measurements of metabolic activities of neutrophils. The removal of necrotic tissue has been found to promote better wound healing caused by thermal exposure. No reliable evidence has been obtained on QC or QECD abilities to significantly accelerate the burn wound epithelialization. The square of the secondary wound scab covered the damaged skin surface has been found to be decreased in the first group of animals on the 14th day followed by the exposure while the rate of wound epithelialization has been found to be increased in the second group of animals at the final stage of wound healing under the treatment with substances being investigated. As a result the ability of QE and QECD to normalize the white blood cell differential, phagocytic and metabolic activities of neutrophils recruited to a wound and neutrophil blood levels has been found.

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The authors declare that there are no conflicts of interest present.