vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2019-16-2-166-174vestim-493Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEЗмяненне гісталагічнай структуры селязёнкі пацукоў пад уздзеяннем прэднізалона і яго камбінацыі з вітамінам DHistological changes in the spleen of rats under the influence of prednisolone and its combination with vitamin DАстроўскіА. А.AstrowskiA. A.

Островский Александр Александрович – д-р мед. наук, профессор, вед. науч. сотрудник

БЛК, 50, 230030, г. Гродно

Alexander A. Astrowski – D. Sc. (Med.), Professor, Leading researcher

50, BLK, 230030, Grodno

astrowski@gmail.comМаксімчыкЮ. З.MaksimchykYu. Z.

Максимчик Юрий Зигмундович – науч. сотрудник

БЛК, 50, 230030, г. Гродно

Yury Z. Maksimchyk – Researcher

50, BLK, 230030, Grodno

y.maksimchyk@ibiochemistry.byГурыновічВ. А.GurinovichV. A.

Гуринович Валерий Александрович – канд. биол. наук, вед. науч. сотрудник

БЛК, 50, 230030, г. Гродно

Valery A. Gurynovich – Ph. D. (Biol.), Leading researcher

50, BLK, 230030, Grodno

val@bioch.basnet.byАстроўскаяА. Б.AstrowskajaA. B.

Островская Оксана Борисовна – канд. биол. наук, ст. науч. сотрудник

ул. Горького, 80, 230009, г. Гродно

Aksana B. Astrowskaja – Ph. D. (Biol.), Senior researcher

80, Gorky Str., 230009, Grodno

astrowskaja@gmail.comМайсяёнакА. Г.MoiseenokA. G.

Мойсеёнок Андрей Георгиевич – член-корреспондент, д-р биол. наук, профессор, заведующий отделом

БЛК, 50, 230030, г. Гродно

Andrey G. Moiseenok – Correspondent Member, D. Sc. (Biol.), Professor, Head of the Department

50, BLK, 230030, Grodno

andrey.moiseenok@tut.byІнстытут біяхіміі біялагічна актыўных злучэнняў НАН БеларусіInstitute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of BelarusГродзенскі дзяржаўны медыцынскі ўніверсітэтGrodno State Medical University201930062019162166174Copyright © Астроўскі А.А., Максімчык Ю.З., Гурыновіч В.А., Астроўская А.Б., Майсяёнак А.Г., 20192019Астроўскі А.А., Максімчык Ю.З., Гурыновіч В.А., Астроўская А.Б., Майсяёнак А.Г.Astrowski A.A., Maksimchyk Y.Z., Gurinovich V.A., Astrowskaja A.B., Moiseenok A.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/493

Изучена возможность использования витамина D для нормализации гистологической структуры селезенки лабораторных крыс, измененной под воздействием преднизолона. Животным ежедневно внутрижелудочно на протяжении 3 недель вводили либо физиологический раствор, либо преднизолон в дозе 5 мг/кг массы, либо преднизолон в комбинации с витамином D (в дозе 800 МЕ/кг). Результаты проведенного морфометрического анализа показали, что введение преднизолона ведет к значительному уменьшению размеров селезенки, объема белой пульпы и размеров герминативных центров лимфатических фолликулов в ее составе и к увеличению плотности размещения мегакариоцитов в красной пульпе органа. Указанные эффекты преднизолона в различной степени нивелируются введением витамина D, в частности наблюдается тенденция к нормализации размеров герминативных центров селезенки. Полученные результаты свидетельствуют о целесообразности дальнейшего изучения возможных механизмов коррекции витамином D иммунологической и гемопоэтической функций организма млекопитающих, измененных введением глюкокортикоидов.

The possibility of using vitamin D to normalize the histological structure of the spleen changed under the influence of prednisolone in rats was studied. The animals were subjected to the intragastric administration of saline and either prednisolone (5 mg/kg b. w.) or its combination with vitamin D (800 IU/kg) daily for 3 weeks. The results of morphometric analysis of spleen slices reveal that the administration of prednisolone leads to a significant decrease in spleen sizes, white pulp volume, and in sizes of germinal centers of lymphatic follicles within the white pulp, and to an increase in the number of megakaryocytes in the red pulp. Vitamin D alleviates histological changes due to the prednisolone treatment, in particular the substantial restoration in sizes of germinal centers in the spleen has been found. The data obtained suggest the benefits of further studies of possible mechanisms of vitamin D to normalize immunological and hematopoietic functions in subjects subjected to glucocorticoid treatment.

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The authors declare that there are no conflicts of interest present.