vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2018-15-4-405-413vestim-455Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEПрименение маркеров активации и дегрануляции базофилов для диагностики пыльцевой аллергииApplication of basophil activation and degranulation markers for diagnosis of pollen allergyРомановаИ. В.RamanavaI. U.

науч. сотрудник

Researcher

romanovairavlad@gmail.comГончаровА. Е.HancharouA. Y.

канд. мед. наук, директор

Ph. D. (Med.), Director

andrei.hancharou@gmail.comДудареваН. И.DudaravaN. I.

канд. мед. наук, заведующий отделением

Ph. D. (Med.), Head of the Department

lpu10gkb@rambler.ruРеспубликанский научно-практический центр эпидемиологии и микробиологии, МинскRepublican Research and Practical Center for Epidemiology and Microbiology, MinskИнститут биофизики и клеточной инженерии НАН Беларуси, МинскInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk10-я городская клиническая больница, Минск10th City Clinical Hospital, Minsk201814012019154405413Copyright © Романова И.В., Гончаров А.Е., Дударева Н.И., 20192019Романова И.В., Гончаров А.Е., Дударева Н.И.Ramanava I.U., Hancharou A.Y., Dudarava N.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/455

В настоящее время известно несколько маркеров активации и дегрануляции базофилов, применяемых в тесте активации базофилов, однако активно используются лишь CD63 и CD203c. С учетом возможности использования и клинической значимости в работе изучены маркеры активации и дегрануляции CD11b, CD13, CD63, CD69, CD107a, CD164, CD203c и CD300a. Показано, что маркер дегрануляции CD107a и маркер активации CD11b обладают высокой чувствительностью и специфичностью и могут применяться для диагностики гиперчувствительности к пыльцевым аллергенам.

There are several basophil activation and degranulation markers for basophil activation test discovered in recent years. However, only CD63 and CD203c are actively used. The activation and degranulation markers (CD11b, CD13, CD63, CD69, CD107a, CD164, CD203c and CD300a) were characterized from the point of view of the possibility to use for BAT and their clinical efficiency. It has been shown that the degranulation marker CD107a and the activation marker CD11b have high sensitivity and specificity and can be used to diagnose hypersensitivity to pollen allergens.

аллергиябазофилымаркерыактивацияпроточная цитометрияallergybasophilsmarkersactivationflow cytometryReferencesBasophil activation testing in diagnosis and monitoring of allergic disease – an overview / B. Eberlein [et al.] // Allergo J. Inter. – 2016. – Vol. 25, N 4. – P. 106–113. https://doi.org/10.1007/s40629-016-0116-2Eberlein B., Santos A. F., Mayorga C., Nopp A., Ferrer M., Rouzaire P., Ebo D., Sabato V., Sanz M. L., Pecaric-Petkovic T., Patil S. U., Hausmann O. V., Shreffler W. G., Korosec P., Knol E. F., Hoffmann H. J. Basophil activation testing in diagnosis and monitoring of allergic disease – an overview. Allergo Journal International, 2016, vol. 25, no. 4, pp. 106–113. https://doi.org/10.1007/s40629-016-0116-2Battye, F. Flow cytometry Consulting [Electronic resource] / F. Battye. – Mode of access : http://www.frankbattye. com.au/Weasel/index.html. – Date of access : 19.06.2016.Battye F. Flow cytometry Consulting. Available at: http://www.frankbattye.com.au/Weasel/index.html (accessed 19.06.2016).Fawcett, T. An introduction to ROC analysis / T. Fawcett // Pattern Recogn. Lett. – 2006. – Vol. 27, N 8. – P. 861–874. https://doi.org/10.1016/j.patrec.2005.10.010Fawcett T. An introduction to ROC analysis. Pattern Recognition. Letters, 2006, vol. 27, no. 8, pp. 861–874. https://doi.org/10.1016/j.patrec.2005.10.010Eusebi, P. Diagnostic accuracy measures / P. Eusebi // Cerebrovasc. Dis. – 2013. – Vol. 36, N 4. – P. 267–272. https://doi.org/10.1159/000353863Eusebi P. Diagnostic accuracy measures. Cerebrovascular Diseases, 2013, vol. 36, no. 4, pp. 267–272. https://doi.org/ 10.1159/000353863Романова, И. В. Оптимизация условий проведения теста активации базофилов / И. В. Романова, А. Е. Гончаров, Н. И. Дударева // Вес. Нац. акад. навук Беларусi. Сер. мед. навук. – 2017. – № 4. – С. 48–59.Romanova I. V., Goncharov A. E., Dudareva N. I. Optimization of basophil activation test technical procedure. Vestsі Natsyyanal’nai akademіі navuk Belarusі. Seryya medytsynskіkh navuk = Proceedings of the National Academy of Sciences of Belarus. Medical series, 2017, no. 4, pp. 48–59 (in Russian).Flow-assisted allergy diagnosis: current application and future perspectives / D. G. Ebo [et al.] // Allergy. – 2006. – Vol. 61, N 9. – P. 1028–1039. https://doi.org/10.1111/j.1398-9995.2006.01039.xEbo D. G., Sainte-Laudy J., Bridts C. H., Mertens C. H., Hagendorens M. M., Schuerwegh A. J., de Clerck L. S., Stevens W. J. Flow-assisted allergy diagnosis: current applications and future perspectives. Allergy, 2006, vol. 61, no. 9, pp. 1028–1039. https://doi.org/10.1111/j.1398-9995.2006.01039.xSyk deficiency in nonreleaser basophils / C. L. Kepley [et al.] // J. Allergy Clin. Immunol. – 1999. – Vol. 104, N 2. – P. 279–284. https://doi.org/10.1016/s0091-6749(99)70367-2Kepley C. L., Youssef L., Andrews R. P., Wilson B. S., Oliver J. M. Syk deficiency in nonreleaser basophils. Journal of Allergy and Clinical Immunology, 1999, vol. 104, no. 2, pp. 279–284. https://doi.org/10.1016/s0091-6749(99)70367-2Романова, И. В. Оптимизация алгоритма гейтирования базофилов на проточном цитометре: многоцветный анализ / И. В. Романова, А. Е. Гончаров, Н. И. Дударева // Вес. Нац. акад. навук Беларусi. Сер. мед. навук. – 2016. – № 4. – С. 15–24.Romanova I. V., Goncharov A. E., Dudareva N. I. Optimization of basophil gating algoruthm on flow cytometry: multicolor analysis. Vestsі Natsyyanal’nai akademіі navuk Belarusі. Seryya medytsynskіkh navuk = Proceedings of the National Academy of Sciences of Belarus. Medical series, 2016, no. 4, pp. 15–24 (in Russian).Basophil activation during pollen season in patients monosensitized to grass pollens / M. Saporta [et al.] // Allergy. – 2001. – Vol. 56, N 5. – P. 442–445. https://doi.org/10.1034/j.1398-9995.2001.056005442.xSaporta M., Kamei S., Persi L., Bousquet J., Arnoux B. Basophil activation during pollen season in patients monosensitized to grass pollens. Allergy, 2001, vol. 56, no. 5, pp. 442–445. https://doi.org/10.1034/j.1398-9995.2001.056005442.xUtility of laboratory testing for the diagnosis of Hymenoptera venom allergy / M. Vachová [et al.] // Allergy Asthma Proc. – 2016. – Vol. 37, N 3. – P. 248–255. https://doi.org/10.2500/aap.2016.37.3934Vachová M., Panzner P., Malkusová I., Hanzlíková J., Vlas T. Utility of laboratory testing for the diagnosis of Hymenoptera venom allergy. Allergy and Asthma Proceedings, 2016, vol. 37, no. 3, pp. 248–255. https://doi.org/10.2500/aap.2016.37.3934Flow-cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to betalactam antibiotics / M. L. Sanz [et al.] // Clin. Exp. Allergy. – 2002. – Vol. 32, N 2. – P. 277–286. https://doi.org/10.1046/ j.1365-2222.2002.01305.xSanz M. L., Gamboa P. M., Antépara I., Uasuf C., Vila L., Garcia-Avilés C., Chazot M., De Weck A. L. Flow-cytometric basophil activation test by detection of CD63 expression in patients with immediate-type reactions to betalactam antibiotics. Clinical Experimental Allergy, 2002, vol. 32, no. 2, pp. 277–286. https://doi.org/10.1046/j.1365-2222.2002.01305.xHuman basophil activation measured by CD63 expression and LTC4 release in IgE-mediated food allergy / D. A. Moneret-Vautrin [et al.] // Ann. Allergy Asthma Immunol. – 1999. – Vol. 82, N 1. – P. 33–40. https://doi.org/10.1016/ s1081-1206(10)62657-9Moneret-Vautrin D. A., Sainte-Laudy J., Kanny G., Frémont S. Human basophil activation measured by CD63 expression and LTC4 release in IgE-mediated food allergy. Annals of Allergy, Asthma and Immunology, 1999, vol. 82, no. 1, pp. 33–40. https://doi.org/10.1016/s1081-1206(10)62657-9Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation / F. Hennersdorf [et al.] // Cell Res. – 2005. – Vol. 15, N 5. – P. 325–335. https://doi.org/10.1038/sj.cr.7290301Hennersdorf F., Florian S., Jakob A., Baumgärtner K., Sonneck K., Nordheim A., Biedermann T., Valent P., Bühring H. J. Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Research, 2005, vol. 15, no. 5, pp. 325–335. https://doi.org/10.1038/sj.cr.7290301Grass pollen allergen-induced surface expression of CD203C, CD63 and CD107A on CRTH2+ basophils: novel biomarkers for monitoring efficacy of allergen-specific immunotherapy / M. Shamji [et al.] // World Allergy Organ. J. – 2012. – Vol. 5, suppl. 2. – P. S49. https://doi.org/10.1097/01.WOX.0000411842.94409.90Shamji M., Layhadi J. A., Cheung D. K. M., Khan S. Q., Phippard D. J., Durham S. Grass pollen allergen-induced surface expression of CD203c, CD63 and CD107a on CRTH2+ basophils: novel biomarkers for monitoring efficacy of allergenspecific immunotherapy. World Allergy Organization, 2012, vol. 5, suppl. 2, p. S49. https://doi.org/10.1097/01.WOX.0000411842.94409.90Bühring, H. J. The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis / H. J. Bühring, A. Streble, P. Valent // Int. Arch. Allergy Immunol. – 2004. – Vol. 133, N 4. – P. 317–329. https://doi.org/10.1159/000077351Bühring H. J., Streble A., Valent P. The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. International Archives of Allergy and Immunology, 2004, vol. 133, no. 4, pp. 317–329. https://doi.org/10.1159/000077351Comparison of basophil activation tests using CD63 or CD203c expression in patients with insect venom allergy / B. Eberlein-König [et al.] // Allergy. – 2006. – Vol. 61, N 9. – P. 1084–1085. https://doi.org/10.1111/j.1398-9995.2006.01122.xEberlein-König B., Varga R., Mempel M., Darsow U., Behrendt H., Ring J. Comparison of basophil activation tests using CD63 or CD203c expression in patients with insect venom allergy. Allergy, 2006, vol. 61, no. 9, pp. 1084–1085. https://doi.org/10.1111/j.1398-9995.2006.01122.xComparison of two basophil activation markers CD63 and CD203c in the diagnosis of amoxicillin allergy / N. Abuaf [et al.] // Clin. Exp. Allergy. – 2008. – Vol. 38, N 6. – P. 921–928. https://doi.org/10.1111/j.1365-2222.2008.02960.xAbuaf N., Rostane H., Rajoely B., Gaouar H., Autegarden J. E., Leynadier F., Girot R. Comparison of two basophil activation markers CD63 and CD203c in the diagnosis of amoxicillin allergy. Clinical and Experimental Allergy, 2008, vol. 38, no. 6, pp. 921–928. https://doi.org/10.1111/j.1365-2222.2008.02960.xMarked improvement of the basophil activation test by detecting CD203c instead of CD63 / R. Boumiza [et al.] // Clin. Exp. Allergy. – 2003. – Vol. 33, N 2. – P. 259–265. https://doi.org/10.1046/j.1365-2222.2003.01594.xBoumiza R., Monneret G., Forissier M.-F., Savoye J., Gutowski M.-C., Powell W. S., Bienvenu J. Marked improvement of the basophil activation test by detecting CD203c instead of CD63. Clinical and Experimental Allergy, 2003, vol. 33, no. 2, pp. 259–265. https://doi.org/10.1046/j.1365-2222.2003.01594.xValidation of basophil CD164 upregulation for pollen allergy diagnosis / A. Wolanczyk-Medrala [et al.] // Arch. Immunol. Ther. Exp. – 2010. – Vol. 58, N 6. – P. 459–465. https://doi.org/10.1007/s00005-010-0104-zWolanczyk-Medrala A., Barg W., Liebhart J., Panaszek B., Nadobna G., Litwa M., Gogolewski G., Medrala W. Validation of basophil CD164 upregulation for pollen allergy diagnosis. Archivum Immunologiae et Therapia Experimentalis, 2010, vol. 58, no. 6, pp. 459–465. https://doi.org/10.1007/s00005-010-0104-zThe effects of dasatinib on IgE receptor-dependent activation and histamine release in human basophils / M. Kneidinger [et al.] // Blood. – 2008. – Vol. 111, N 6. – P. 3097–3107. https://doi.org/10.1182/blood-2007-08-104372Kneidinger M., Schmidt U., Rix U., Gleixner K. V., Vales A., Baumgartner C., Lupinek C., Weghofer M., Bennett K. L., Herrmann H., Schebesta A., Thomas W. R., Vrtala S., Valenta R., Lee F. Y., Ellmeier W., Superti-Furga G., Valent P. The effects of dasatinib on IgE receptor-dependent activation and histamine release in human basophils. Blood, 2008, vol. 111, no. 6, pp. 3097–3107. https://doi.org/10.1182/blood-2007-08-104372Recombinant allergens promote expression of aminopeptidase-n (CD13) on basophils in allergic patients / K. Sonneck [et al.] // Int. J. Immunopathol. Pharmacol. – 2008. – Vol. 21, N 1. – P. 11–21. https://doi.org/10.1177/039463200802100103Sonneck K., Baumgartner C., Rebuzzi L., Marth K., Chen K. W., Hauswirth A. W., Florian S., Vrtala S., Bühring H. J., Valenta R., Valent P. Recombinant allergens promote expression of aminopeptidase-n (CD13) on basophils in allergic patients. International Journal of Immunopathology and Pharmacology, 2008, vol. 21, no. 1, pp. 11–21. https://doi.org/10.1177/039463200802100103Activation markers of human basophils: CD69 expression is strongly and preferentially induced by IL-3 / C. Yoshimura [et al.] // J. Allergy Clin. Immunol. – 2002. – Vol. 109, N 5. – P. 817–823. https://doi.org/10.1067/mai.2002.123532Yoshimura C., Yamaguchi M., Iikura M., Izumi S., Kudo K., Nagase H., Ishii A., Walls A.F., Ra C., Iwata T., Igarashi T., Yamamoto K., Hirai K. Activation markers of human basophils: CD69 expression is strongly and preferentially induced by IL-3. Journal of Allergy and Clinical Immunology, 2002, vol. 109, no. 5, pp. 817–823. https://doi.org/10.1067/mai.2002.123532Bochner, B. S. Altered surface expression of CD11 and Leu 8 during human basophil degranulation / B. S. Bochner, S. A. Sterbinsky // J. Immunol. – 1991. – Vol. 146, N 7. – P. 2367–2373.Bochner B. S., Sterbinsky S. A. Altered surface expression of CD11 and Leu 8 during human basophil degranulation. Journal of Immunology, 1991, vol. 146, no. 7, pp. 2367–2373.Effects of prostaglandin D(2) and 5-lipoxygenase products on the expression of CD203c and CD11b by basophils / G. Monneret [et al.] // J. Pharmacol. Exp. Ther. – 2005. – Vol. 312, N 2. – P. 627–634. https://doi.org/10.1124/jpet.104.074823Monneret G., Boumiza R., Gravel S., Cossette C., Bienvenu J., Rokach J., Powell W. S. Effects of prostaglandin D(2) and 5-lipoxygenase products on the expression of CD203c and CD11b by basophils. Journal of Pharmacology and Experimental Therapeutics, 2005, vol. 312, no. 2, pp. 627–634. https://doi.org/10.1124/jpet.104.074823MacGlashan Jr., D. Marked differences in the signaling requirements for expression of cd203c and cd11b versus cd63 expression and histamine release in human basophils / D. MacGlashan Jr. // Int. Arch. Allergy Immunol. – 2012. – Vol. 159, N 3. – P. 243–252. https://doi.org/10.1159/000332150MacGlashan Jr. D. Marked differences in the signaling requirements for expression of cd203c and cd11b versus cd63 expression and histamine release in human basophils. International Archives of Allergy and Immunology, 2012, vol. 159, no. 3, pp. 243–252. https://doi.org/10.1159/000332150Human basophils express the inhibitory receptor CD300a (IRp60) / V. Sabato [et al.] // J. Allergy Clin. Immunol. – 2011. – Vol. 127, N 2. – P. AB75. https://doi.org/10.1016/j.jaci.2010.12.310Sabato V., Verweij M., Bridts C., De Clerck L., Stevens W., Schiavino D., Ebo D. Human Basophils express the inhibitory receptor CD300a (IRp60). Journal of Allergy and Clinical Immunology, 2011, vol. 127, no. 2, p. AB75. https://doi.org/10.1016/ j.jaci.2010.12.310Mechanism of phosphatidylserine inhibition of IgE/FcεRI-dependent anaphylactic human basophil degranulation via CD300a / V. Sabato [et al.] // J. Allergy Clin. Immunol. – 2014. – Vol. 134, N 3. – P. 734–737. https://doi.org/10.1016/ j.jaci.2014.03.029Sabato V., Boita M., Shubber S., Bridts C. H., Shibuya A., De Clerck L. S., Falcone F. H., Ebo D. G. Mechanism of phosphatidylserine inhibition of IgE/FcεRI-dependent anaphylactic human basophil degranulation via CD300a. Journal of Allergy and Clinical Immunology, 2014, vol. 134, no. 3, pp. 734–737. https://doi.org/10.1016/j.jaci.2014.03.029

The authors declare that there are no conflicts of interest present.