vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2018-15-3-293-305vestim-448Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEКОРРЕКЦИЯ РАССТРОЙСТВ РЕПРОДУКТИВНОЙ ФУНКЦИИ САМЦОВ КРЫС С ПОМОЩЬЮ НАНОЧАСТИЦ ОРТОВАНАДАТА ГАДОЛИНИЯCORRECTION OF DISORDERS OF THE REPRODUCTIVE FUNCTION OF MALE RATS USING GADOLINIUM ORTHOVANADATE NANOPARTICLESБелкинаИ. О.BelkinaI. O.

Белкина Инна Олеговна – мл. науч. сотрудник

ул.  Алчевских, 10, 61002, г. Харьков

Inna O. Belkina – Junior researcher

10, Alchevsky, 61002, Kharkiv

inna-bel@mail.ruКарпенкоН. А.KarpenkoN. A.

Карпенко Нина Алексеевна – канд.  биол.  наук, ст. науч. сотрудник, заведующий лабораторией

ул.  Алчевских, 10, 61002, г. Харьков

Nina A. Karpenko – Ph.  D. (Biol.), Senior researcher, Head of the Laboratory

10, Alchevsky, 61002, Kharkiv

nina.а.karpenko@gmail.com

КореневаЕ. М.KorenevaE. M.

Коренева Евгения Михайловна – канд. биол. наук, ст. науч. сотрудник

ул. Алчевских, 10, 61002, г. Харьков

Evgenia M. Koreneva – Ph. D. (Biol.), Senior researcher

10, Alchevsky, 61002, Kharkiv

eugenia_ koreneva@ukr.net

СмоленкоН. П.SmolenkoN. P.

Смоленко Наталья Павловна – канд. биол. наук, ст. науч. сотрудник

ул. Алчевских, 10, 61002, г. Харьков

Natalia P. Smolenko – Ph. D. (Biol.), Senior researcher

10, Alchevsky, 61002, Kharkiv

smtaska@ukr.netЧистяковаЭ. Е.ChistyakovaE. E.

Чистякова Элина Евгеньевна – канд. биол. наук, ст. науч. сотрудник

ул. Алчевских, 10, 61002, г. Харьков

Elina E. Chistyakova – Ph. D. (Biol.), Senior researcher

10, Alchevsky, 61002, Kharkiv

chistelina@i.uaКлочковВ. К.KlochkovV. K.

Клочков Владимир Кириллович – канд. хим. наук, ст. науч. сотрудник

пр. Науки, 60, 61072, г. Харьков

Vladimir K. Klochkov – Ph. D. (Chem.), Senior researcher

60, Nauky Ave, 61072, Kharkiv

 

Институт проблем эндокринной патологии им. В. Я. Данилевского НАМН УкраиныV. Danilevsky Institute for Endocrine Pathology Problems of the National Academy of Medical Sciences of UkraineИнститут сцинтилляционных материалов НАН УкраиныInstitute for Scintillation Materials of the National Academy of Sciences of Ukraine201816092018153293305Copyright © Белкина И.О., Карпенко Н.А., Коренева Е.М., Смоленко Н.П., Чистякова Э.Е., Клочков В.К., 20182018Белкина И.О., Карпенко Н.А., Коренева Е.М., Смоленко Н.П., Чистякова Э.Е., Клочков В.К.Belkina I.O., Karpenko N.A., Koreneva E.M., Smolenko N.P., Chistyakova E.E., Klochkov V.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/448

Установлено, что самцы крыс, которые в неонатальный период (с 3-х по 22-е сутки жизни) испытывали эмоциональный стресс и получали избыточное количество фитоэстрогенов с молоком матери, в 10-месячном возрасте имеют избыточную массу тела, что обусловлено увеличением массы висцерального жира, изменением белкового обмена и проатерогенными изменениями липидного обмена (повышением уровня общего холестерина и триглицеридов), нарушением про/антиоксидантного баланса. Кроме того, у этих самцов отмечался сниженный уровень тестостерона и имелись нарушения сперматогенеза (меньше общая концентрация сперматозоидов и меньшая концентрация морфологически нормальных гамет), проявляющиеся в образовании дефектных сперматозоидов. Обнаружены также нарушения полового поведения и снижение способности к оплодотворению самок. При наступлении беременности у самок более чем в 2 раза увеличивались общие внутриутробные потери. Вследствие таких изменений показатель репродуктивного потенциала (Фi ) у самцов был снижен вдвое по сравнению с контрольным уровнем.

Исследована эффективность применения наночастиц ортованадата гадолиния, активированных европием (НЧ GdVO4 :Eu3+), в составе гидрозоля или фармакологической композиции (в дозе 0,33 мг/кг массы тела в течение 70 сут) для коррекции расстройств мужской репродуктивной функции. В качестве референтных изучены препараты трибестан в дозе 68 мг/кг и спеман в дозе 168 мг/кг. Положительное влияние НЧ GdVO4 :Eu3+ на репродуктивную функцию самцов крыс с экспериментальной репродуктопатией, проявляющееся в нормализации сперматогенеза, уровня тестостерона, содержания триглицеридов, общего холестерина, аргинина в сыворотке крови, активности аланинаминотрансферазы в печени, фертильности и плодовитости, уменьшении эмбриональной смертности способствовало нормализации расчетного показателя репродуктивного потенциала. Увеличение массы гипофиза и повышение гонадотропной активности гипофизов самцов, получавших НЧ GdVO4 :Eu3+, может указывать на возможное действие НЧ на центральный механизм регуляции репродуктивной функции. Несколько большая биологическая активность НЧ в составе гидрозоля может объясняться введением в состав фармакологической композиции вспомогательных веществ, влияющих на физико-химические свойства НЧ и их биологическую активность.

Male rats were exposed to emotional stress and had an excessive amount of phytoestrogens with the mother’s milk (from 3 to 22 days of life) and were examined at an age of 10 months. Animals had an excess body weight through an increased weight of visceral fat, changes in protein and lipid metabolism (increased total cholesterol and triglycerides), impaired pro/antioxidant balance. These males had a decreased level of testosterone and impaired spermatogenesis (less total sperm concentration and less concentration of morphologically normal gametes) with the formation of defective spermatozoa. Also, the violations of the sexual behavior and the worst fertilization of females were found. More than a two-fold increase of intrauterine losses in these pregnant females was noted. Due to such changes, the reproductive potential (Fi ) in these males was reduced by half compared to the control level.

The effectiveness of application of gadolinium orthovanadate nanoparticles activated by europium (NPs GdVO4 :Eu3+), as part of a hydrogel or a pharmacological composition (0.33 mg/kg of body mass, 70 days) for correction of male reproductive function disorders was studied. As reference preparations, tribestane (68 mg/kg) or spemane (168 mg/kg) was used. After the treatment, the results of spermatogenesis, testosterone production and fertility of the main group of males became better. Due to the reduction of embryonic losses, the reproductive potential index was normalized. NPs normalized the level of triglycerides, total cholesterol, arginine in the serum, as well as the transaminase activity in the liver. An increase in the weight and gonadotropic activity of hypophysis of the treated males were found, indicating a possible effect of NPs on the central regulatory mechanism of the reproductive function. The higher biological activity of NPs in the hydrosol composition was found, which can be explained by the introduction of auxiliary substances into the pharmacological composition that affects the physicochemical properties of NPs GdVO4 :Eu3+ and their biological activity.

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The authors declare that there are no conflicts of interest present.