vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestim-404Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEЗМЯНЕННЕ ГІСТАЛАГІЧНАЙ СТРУКТУРЫ НАДНЫРКАВЫХ ЗАЛОЗ ПАЦУКОЎ ПАД УЗДЗЕЯННЕМ ПРЭДНІЗАЛОНА І ЯГО КАМБІНАЦЫІ З ВІТАМІНАМ DCHANGES IN THE HISTOLOGICAL STRUCTURE OF RAT ADRENAL GLANDS EXPOSED TO PREDNISOLONE AND ITS COMBINATION WITH VITAMIN DОстровскийА. А.AstrowskiA. A.

д-р мед. наук, профессор, вед. науч. сотрудник

БЛК, 50, 230030

D. Sc. (Med.), Professor, Leading researcher

50, BLK, 230030

astrowski@gmail.comГурыновічВ. А.GurynovichV. A.

канд. биол. наук, вед. науч. сотрудник

БЛК, 50, 230030

Ph. D. (Biol.), Senior researcher

50, BLK, 230030

val@bioch.basnet.byМаксiмчыкЮ. З.MaksimchykYu. Z.

мл. науч. сотрудник

БЛК, 50, 230030

Researcher

50, BLK, 230030

yurijmaks@yahoo.comАстроўскаяА. Б.AstrowskayaA. B.

канд. биол. наук, ст. науч. сотрудник

ул. Горького, 80, 230009

Ph. D. (Biol.), Senior researcher

80, Gorky Str., 230009

astrowskaja@gmail.comАрэхаўС. Д.ArekhauS. D.

канд. мед. наук, доцент

ул. Горького, 80, 230009

Ph. D. (Med.), Assistant Professor

80, Gorky Str., 230009

arekhau57@gmail.comМайсяёнакА. Г.MoiseenokA. G.

член-корреспондент, д-р биол. наук, профессор, заведующий отделом

БЛК, 50, 230030

Correspondent Member, D. Sc. (Biol.), Professor, Head of the Department

50, BLK, 230030

andrey.moiseenok@tut.byИнститут биохимии биологически активных соединений НАН БеларусиInstitute of Biochemistry of Biologically Active Compounds of the National Academy of Sciences of Belarus, GrodnoГродненский государственный медицинский университетGrodno State Medical University, Grodno201710122017047785Copyright © Островский А.А., Гурыновіч В.А., Максiмчык Ю.З., Астроўская А.Б., Арэхаў С.Д., Майсяёнак А.Г., 20172017Островский А.А., Гурыновіч В.А., Максiмчык Ю.З., Астроўская А.Б., Арэхаў С.Д., Майсяёнак А.Г.Astrowski A.A., Gurynovich V.A., Maksimchyk Y.Z., Astrowskaya A.B., Arekhau S.D., Moiseenok A.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/404

Изучено одновременное воздействие преднизолона и витамина D на гистологическую структуру надпочечниковых желез лабораторных крыс. Животным ежесуточно в течение 3 недель внутрижелудочно вводили либо физраствор, либо преднизолон в дозе 5 мг/кг массы тела, либо преднизолон в комбинации с витамином D в дозе 800 МЕ/кг. Надпочечники фиксировали в смеси формалин–спирт–уксусная кислота, заливали в парафин, делали серийные срезы, которые окрашивали гематоксилином и эозином. Показано, что введение витамина D нормализовало пролиферацию и апоптоз клеток коры надпочечниковых желез, измененных под влиянием преднизолона, однако не обеспечивало восстановления размеров желез. Введение витамина D вместе с преднизолоном способствовало накоплению липофусцина в сетчатой зоне коры надпочечников. 

The combined effects of prednisolone and vitamin D on the rat adrenal histological structure was studied. The animals were administered with 5 mg/kg of either saline, or prednisolone intragastrically, daily over 3 weeks, or prednisolone in combination with vitamin D (at a dose of 800 IU/kg). The adrenals were fixed in a mixture of formalin-ethanol-acetic acid, embedded in paraffin and serial sections were made which were stained with hematoxylin and eosin. It was found that although vitamin D was beneficial for normalization of proliferation and apoptosis of adrenal cortical cells changed under the exposure to prednisolone, it did not contribute to recovering of the gland size. Vitamin D in combination with prednisolone contributed to storing of lipofuscin in the reticular layer of the adrenal cortex. 

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The authors declare that there are no conflicts of interest present.