vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestim-400Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEОПТИМИЗАЦИЯ УСЛОВИЙ ПРОВЕДЕНИЯ ТЕСТА АКТИВАЦИИ БАЗОФИЛОВOPTIMIZATION OF BASOPHIL ACTIVATION TEST CONDITIONSРомановаИ. В.RamanavaI. U.

науч. сотрудник

ул. Филимонова, 23, 220114

Researcher

23 Filimonov Str., 220114

romanovairavlad@gmail.comГончаровА. Е.HancharouA. Y.

канд. мед. наук, заведующий лабораторией

ул. Филимонова, 23, 220114

Ph. D. (Med.), Head of the Laboratory

23, Filimonov Str., 220114

andrei.hancharou@gmail.comДудареваН. И.DudaravaN. I.

канд. мед. наук, заведующий отделением

ул. Уборевича, 73, 220096

Ph. D. (Med.), Head of the Department

73, Uborevich Str., 220096

lpu10gkb@rambler.ruРеспубликанский научно-практический центр эпидемиологии и микробиологии, МинскThe Republican Research and Practical Center for Epidemiology and Microbiology, Minsk10-я городская клиническая больница, Минск10th City Clinical Hospital, Minsk201709122017044859Copyright © Романова И.В., Гончаров А.Е., Дударева Н.И., 20172017Романова И.В., Гончаров А.Е., Дударева Н.И.Ramanava I.U., Hancharou A.Y., Dudarava N.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/400

Оптимизированы условия постановки и учета теста активации базофилов (ТАБ) для лаборатор- ной диагностики IgE-опосредованной аллергии. Установлены оптимальные условия хранения венозной крови для проведения ТАБ: не более 4 ч при температуре 18–25 °С. Адаптировано и обосновано преимущество применения форбол-12-миристат-13-ацетата в качестве неспецифического положительного контроля. Исследована экспрессия молекул, относящихся к разным функциональным классам, на неактивированных и активированных базофилах. Показано, что молекулы CD11b, CD13, CD69, CD107a, CD164 и CD300a усиливают экспрессию на базофилах под влиянием антитела к IgE и могут быть использованы в качестве дополнительных маркеров при постановке ТАБ. 

The basophil activation test (BAT) is a perspective method for allergy diagnosis. However, the optimization and standardization of the method are required. The optimal conditions for the peripheral blood storage for BAT were established: the blood should be stored at room temperature (18–25 °C), and a test should be performed within 4 h after blood collection. The optimal positive control – phorbol 12-myristate 13-acetate was chosen due to effective basophil activation and low cost. The expression of molecules on both unstimulated and activated basophils was studied. It was shown that CD11b, CD13, CD69, CD107a, CD164 and CD300a molecules may be used to improve the BAT efficiency. 

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The authors declare that there are no conflicts of interest present.