vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestim-395Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEПОДАВЛЕНИЕ ЦИТОТОКСИЧЕСКОЙ ФУНКЦИИ CD8+ Т-ЛИМФОЦИТОВ И ЕСТЕСТВЕННЫХ КИЛЛЕРНЫХ КЛЕТОК МЕЗЕНХИМАЛЬНЫМИ СТВОЛОВЫМИ КЛЕТКАМИ ОБОНЯТЕЛЬНОЙ ВЫСТИЛКИ ПОЛОСТИ НОСАSUPPRESSION OF CD8+ T-LYMPHOCYTES AND NATURAL KILLER CELLS CYTOTOXIC FUNCTION BY HUMAN OLFACTORY MUCOSA-DERIVED MESENCHYMAL STEM CELLSАнтоневичН. Г.AntonevichN. H.

науч. сотрудник

ул. Филимонова, 23, 220114, г. Минск

Researcher

23, Filimonov Str., 220114

antonevich.n@gmail.comГончаровА. Е.HancharouA. Y.

канд. мед. наук, заведующий лабораторией

ул. Филимонова, 23, 220114, г. Минск

Ph. D. (Med.), Head of the Laboratory

23, Filimonov Str., 220114

andrei.hancharou@gmail.comБущикО. В.BuschikO. V.

мл. науч. сотрудник

ул. Филимонова, 23, 220114, г. Минск

Junior Researcher

23, Filimonov Str., 220114

oksanabuschik@gmail.comРындаЕ. Г.RyndaA. G.

мл. науч. сотрудник

ул. Филимонова, 23, 220114, г. Минск, Республика Беларусь). E-mail: 

Junior Researcher

23, Filimonov Str., 220114

alenarynda@gmail.comЧеканВ. Л.ChekanV. L.

канд. мед. наук, доцент

ул. Сухая, 8, 220004, г. Минск

Ph. D. (Med.), Assistant Professor

8, Sukhaya Str., 220004

lor3377@yandex.ruСтринкевичЭ. А.StrinkevichE. A.

канд. мед. наук, ассистент

ул. Сухая, 8, 220004, г. Минск

Ph. D. (Med.), Assistant

8, Sukhaya Str., 220004

lorkafedra@tut.byРеспубликанский научно-практический центр эпидемиологии и микробиологии, МинскRepublican Research and Practical Center for Epidemiology and Microbiology, MinskБелорусская государственная медицинская академия последипломного образования, МинскBelarusian Medical Academy of Postgraduate Education, Minsk20170912201704716Copyright © Антоневич Н.Г., Гончаров А.Е., Бущик О.В., Рында Е.Г., Чекан В.Л., Стринкевич Э.А., 20172017Антоневич Н.Г., Гончаров А.Е., Бущик О.В., Рында Е.Г., Чекан В.Л., Стринкевич Э.А.Antonevich N.H., Hancharou A.Y., Buschik O.V., Rynda A.G., Chekan V.L., Strinkevich E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/395

В работе изучены иммуномодулирующие свойства мезенхимальных стволовых клеток обонятельной выстилки (МСК ОВ) полости носа человека в отношении функциональной активности цитотоксических Т-лимфоцитов (ЦТЛ) и естественных киллерных клеток (ЕКК) – клеточно-опосредованной цитотоксичности. Впервые показано, что иммуномодулирующий эффект МСК ОВ на ЦТЛ и ЕКК проявляется в подавлении механизмов реализации цитотоксической функции (снижении продукции перфорина и гранзима В, уменьшении экспрессии CD107a), что снижает способность эффекторных клеток уничтожать клетки-мишени. Полученные данные могут быть использованы при разработке биомедицинских клеточных продуктов на основе МСК ОВ для лечения заболеваний, в иммунопатогенезе которых ведущая роль принадлежит цитотоксической активности ЦТЛ и ЕКК. 

In this study, the immunomodulatory effects of human olfactory mucosa-derived stem cells (hOM-MSCs) against the cell-mediated cytotoxic activity of cytotoxic T-lymphocytes (CTLs) and natural killer cells (NKCs) are evaluated. It has been shown that the immunomodulatory activity is realized through a suppression of cytotoxic mechanisms (decrease in the expression of perforin, granzime B, CD107a), which eventually leads to a reduced ability to induce apoptosis in target tumor cells. The obtained results can be applied for development of biomedical cell products based on hOM-MSCs to treat diseases in which the leading role is played by the cytotoxic activity of CTL and NKCs. 

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The authors declare that there are no conflicts of interest present.