vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestim-374Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEВЛИЯНИЕ СИНТЕТИЧЕСКОГО АНАЛОГА ФРАГМЕНТА АРГИНИН-ВАЗОПРЕССИНА НА ПРОЦЕСС ОБУЧЕНИЯ КРЫСINFLUENCE OF THE SYNTHETIC ANALOG OF THE ARGININE-VASOPRESSIN FRAGMENT ON RAT TRAININGБеляковаА. С.BelyakovaA. S.

инженер-лаборант

alixletter@yandex.ruСинюшинА. А.SinjushinA. A.

канд. биол. наук, доцент

asinjushin@mal.ruВоскресенскаяО. Г.VoskresenskayaO. G.

канд. биол. наук, ст. науч. сотрудник

voskresenskaya05@mail.ruГолубовичВ. П.GolubovichV. P.

д-р хим. наук, про- фессор

golubovich@iboch.bas-net.byКаменскийА. А.KamenskyA. A.

д-р биол. наук, профессор, заведующий кафедрой

voskresenskaya05@mail.ruМосковский государственный университет имени М. В. ЛомоносоваM. V. Lomonosov Moscow State UniversityИнститут биоорганической химии НАН БеларусиInstitute of Bioorganical Chemistry of the National Academy of Sciences of Belarus20170908201702104116Copyright © Белякова А.С., Синюшин А.А., Воскресенская О.Г., Голубович В.П., Каменский А.А., 20172017Белякова А.С., Синюшин А.А., Воскресенская О.Г., Голубович В.П., Каменский А.А.Belyakova A.S., Sinjushin A.A., Voskresenskaya O.G., Golubovich V.P., Kamensky A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/374

Аргинин-вазопрессин – пептидный гормон, имеющий ряд экстрагормональных эффектов. В частности, он оказывает влияние на процессы обучения и выработки устойчивости к стрессовым факторам среды. В настоящей работе изучено влияние синтетического аналога фрагмента аргинин-вазопрессина, Ac-D-SPRG, на процесс обучения крыс. Этот аналог имитирует фрагмент исходного гормона, образующийся при его эндогенном расщеплении. Для повышения устойчивости соединения вещества в его состав введен D-серин. Исследования проведены на половозрелых самцах нелинейных белых крыс с использованием стандартных поведенческих тестов. Показано, что данный препарат не оказывает значимого влияния на обучение при положительном пищевом подкреплении. В тесте на выработку условной реакции пассивного избегания применение тестируемого препарата в больших дозах оказывало положительное действие. Наиболее существенным действие аналога установлено в тесте на выработку условной реакции активного избегания. Главный объект действия аналога – реакция на условный звуковой сигнал. По итогам работы сделан вывод о том, что улучшение обучения животных с помощью используемого аналога тем сильнее, чем более конкретным, дифференцированным и обособленным является условный сигнал. 

Arginine-vasopressin comprises an intriguing peptide hormone having a number of additional effects. It influences the efficiency of training and resistance against stress factors. This work reports the results for the influence of the synthetic analog of the arginine-vasopressin fragment, Ac-D-SPRG on the rat training. A given substance mimics a natural tetrapeptide which results from endogenous lysis of arginine-vasopressin. To make this tetrapeptide more stable in physiological conditions, D-serine was introduced in its sequence. The work was carried out on mature male non-linear rats. The substance appeared to have no influence on the training with positive food reinforcement. In a passive avoidance conditioned reaction test, a substance under test had a positive action in high doses. The most significant effect was recorded in a test of a conditioned reaction of active avoidance of pain stimulus. The main target of the analog action is the reaction to a conditioned sound signal. We concluded that the tested analog improves the animal training with regard to exact, differentiated and expressed conditioned stimulus.

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The authors declare that there are no conflicts of interest present.