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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vestim</journal-id><journal-title-group><journal-title xml:lang="ru">Известия Национальной  академии наук Беларуси. Серия медицинских наук</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of the National Academy of Sciences of Belarus, Medical series</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1814-6023</issn><issn pub-type="epub">2524-2350</issn><publisher><publisher-name>The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vestim-241</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>КЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНА</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>CLINICAL AND EXPERIMENTAL MEDICINE</subject></subj-group></article-categories><title-group><article-title>Векторная конструкция для накопления армированных РНК</article-title><trans-title-group xml:lang="en"><trans-title>Vector construction for production of armored nucleic acids</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Землянский</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Zemlianski</surname><given-names>V. A.</given-names></name></name-alternatives><email xlink:type="simple">labsanvir@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дедюля</surname><given-names>К. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Dziadziulia</surname><given-names>K. L.</given-names></name></name-alternatives><email xlink:type="simple">labsanvir@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Поклонская</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Paklonskaya</surname><given-names>N. V.</given-names></name></name-alternatives><email xlink:type="simple">labsanvir@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Амвросьева</surname><given-names>Т. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Amvrosieva</surname><given-names>T. V.</given-names></name></name-alternatives><email xlink:type="simple">labsanvir@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff xml:lang="ru" id="aff-1"><institution>Республиканский научно-практический центр эпидемиологии и микробиологии</institution><country>Belarus</country></aff><pub-date pub-type="collection"><year>2015</year></pub-date><pub-date pub-type="epub"><day>06</day><month>06</month><year>2016</year></pub-date><volume>0</volume><issue>2</issue><fpage>19</fpage><lpage>22</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Землянский В.А., Дедюля К.Л., Поклонская Н.В., Амвросьева Т.В., 2016</copyright-statement><copyright-year>2016</copyright-year><copyright-holder xml:lang="ru">Землянский В.А., Дедюля К.Л., Поклонская Н.В., Амвросьева Т.В.</copyright-holder><copyright-holder xml:lang="en">Zemlianski V.A., Dziadziulia K.L., Paklonskaya N.V., Amvrosieva T.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vestimed.belnauka.by/jour/article/view/241">https://vestimed.belnauka.by/jour/article/view/241</self-uri><abstract><p>Рассмотрена перспективная технология создания армированных нуклеиновых кислот - псевдовирусных частиц, содержащих устойчивый к действию РНКаз фрагмент РНК. Описан процесс их разработки и получения, перспективы использования в качестве контролей для полимеразной цепной реакции с обратной транскрипцией.</p></abstract><trans-abstract xml:lang="en"><p>A widespread use of RT-PCR as an effective method of RNA-viruses diagnostics has created a need for stable and relevant controls. Unfortunately, controls based on plasmids and vial viruses have several critical disadvantages. The armored RNA technology shows ways to avoid these limitations. Armored RNA is a complex of MS2 bacteriophage coat protein and RNA produced in Escherichia coli by the induction of an expression plasmid that encodes the coat protein and an RNA control sequence. The RNA sequences are protected from RNase digestion within the pseudoviral complex. These particles can work as a control on every stage of PCR virus detection - lysis, purification, revert transcription and amplification. This article describes the process of development and construction of armored RNA for a subsequent use as control for RT-PCR detection of RNA-viruses.</p></trans-abstract></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Землянский В. А., Дедюля К. Л., Амвросьева Т. В. // Современные проблемы инфекционной патологии человека: сб. науч. тр. Минск, 2014. № 7. С. 157-161.</mixed-citation><mixed-citation xml:lang="en">Землянский В. А., Дедюля К. Л., Амвросьева Т. В. // Современные проблемы инфекционной патологии человека: сб. науч. тр. Минск, 2014. № 7. С. 157-161.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Huang Q. et al. // Clin. Chem. 2006. Vol. 52 (7). P. 1446-1448.</mixed-citation><mixed-citation xml:lang="en">Huang Q. et al. // Clin. Chem. 2006. Vol. 52 (7). 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