vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2026-23-3-183-192vestim-1100Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEСравнительное лабораторное исследование методов получения аутологичных тромбоцитарных концентратов: доклинический протокол для лечения периимплантитаA comparative laboratory study of methods for obtaining autologous platelet concentrates: a preclinical protocol for the treatment of peri-implantitishttps://orcid.org/0000-0002-7450-3757РубниковичС. П.RubnikovichS. P.

Рубникович Сергей Петрович – член-корреспондент, д-р мед. наук, профессор, ректор

пр. Дзержинского, 83, 220083, г. Минск

Sergey P. Rubnikovich – Corresponding Member, D. Sc. (Med.), Professor, Rector

83, Dzerzhinsky Ave., 220083, Minsk 

rubnikovichs@mail.ruhttps://orcid.org/0000-0001-7004-3581ПериковаМ. Г.PerikovaM. G.

Перикова Мария Григорьевна – канд. мед. наук, доцент 

ул. Мира, 310, 355017, г. Ставрополь 

Maria G. Perikova – Ph. D. (Med.), Associate Professor 

310, Mira Str., 355017, Stavropol

masha.perikova@yandex.ruhttps://orcid.org/0000-0002-4924-5792СиракС. В.SirakS. V.

Сирак Сергей Владимирович – д-р мед. наук, профес­сор, заведующий кафедрой

ул. Мира, 310, 355017, г. Ставрополь

Sergey V. Sirak – D. Sc. (Med.), Professor, Head of the Department

310, Mira Str., 355017, Stavropol

sergejsirak@yandex.ruБелорусский государственный медицинский университетБеларусьBelarusian State Medical UniversityBelarusСтавропольский государственный медицинский университетРоссияStavropol State Medical UniversityRussian Federation202630082026233183192Copyright © Рубникович С.П., Перикова М.Г., Сирак С.В., 20262026Рубникович С.П., Перикова М.Г., Сирак С.В.Rubnikovich S.P., Perikova M.G., Sirak S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/1100

Настоящее исследование посвящено сравнительному лабораторному анализу различных методов получения аутологичных тромбоцитарных концентратов (АТК) для лечения периимплантита. Цель исследования – лабораторное обоснование оптимальной технологии получения АТК, обеспечивающего максимальные биорегенеративные потенции и минимальный воспалительный ответ при лечении периимплантита.Исследование проводилось на образцах венозной крови, взятой у 30 здоровых доноров (15 мужчин, 15 женщин; возраст – 25–55 лет) без отклонений показателей общего анализа крови. Две группы образцов были получены по разным технологиям: 1-я группа – традиционным методом получения PRP (Platelet-Rich Plasma), 2-я группа – по технологии Cortexil PRP. Полученные АТК изучены с помощью следующих лабораторных методов: атомно-силовая мик­ роскопия (АСМ), подсчет тромбоцитов, лейкоцитов, эритроцитов в каждом слое с использованием автоматического гематологического анализатора, определение концентрации факторов роста и цитокинов – методом ИФА (ELISA).Собранные данные показали преимущество технологии Cortexil PRP, которая обеспечивает оптимальную терапевтическую концентрацию тромбоцитов в PRP, превосходящую традиционную методику PRP по наиболее важным показателям. Так, P/WBC >85 в группе Cortexil – значение, превышающее порог эффективной регенерации (>60); высокий P/R >165 указывает на минимальную эритроцитарную примесь в PRP по технологии Cortexil PRP. АСМ показала, что более 80 % тромбоцитов в Cortexil PRP сохраняли дискоидную форму (признак неактивированного состояния).Качество тромбоцитарного концентрата должно оцениваться по его биологическому профилю, где P/WBC >60 – оптимальная концентрация для подавления воспаления; P/R >120 – для проникновения в кость; >75 % дискоидных тромбоцитов – для контролируемого высвобождения факторов роста; низкий IL-1β/TNF-α – для предотвращения резорбции.

This study presents a comparative laboratory investigation of various methods for obtaining autologous platelet concentrates for the treatment of peri-implantitis.The aim of the study was to provide laboratory validation of an optimal technology for producing autologous platelet concentrates that maximize bioregenerative potential and minimize inflammatory response in the treatment of peri-implantitis.The study was conducted on venous blood samples collected from 30 healthy donors (15 men, 15 women, aged 25–55 years) with no abnormalities in complete blood counts. Two groups of samples were obtained using different technologies: Group 1 – traditional PRP extraction, Group 2 – Cortexil PRP technology. The resulting autologous platelet concentrates were analyzed using the following laboratory methods: atomic force microscopy (AFM); platelet, leukocyte, and erythrocyte counts in each layer using an automated hematology analyzer; and determination of growth factor and cytokine concentrations using ELISA.The obtained data demonstrated the advantage of the Cortexil PRP technology, which provides an optimal therapeutic concentration of platelets in PRP, surpassing the traditional PRP technique in the most important parameters. Thus, the P/WBC ratio in the Cortexil group exceeded 85, surpassing the threshold for effective regeneration (>60); a high P/R >165 indicates minimal red blood cell admixture in PRP using the Cortexil PRP technology. AFM showed that 82 % of platelets in Cortexil PRP retained a discoid shape, a sign of an inactivated state.The quality of platelet concentrate should be assessed by its biological profile, where a P/WBC ratio >60 provides optimal conditions for suppressing inflammation; a P/R ratio >120 is recommended for bone penetration; >75 % discoid platelets are required for controlled release of growth factors; and low IL-1β and TNF-α levels are required to prevent resorption.

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The authors declare that there are no conflicts of interest present.