vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2026-23-2-95-103vestim-1082Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEОценка цитотоксичности антисептика широкого спектра действия на модели гемолиза эритроцитов в эксперименте in vitroEvaluation of the cytotoxicity of a broad-spectrum antiseptic using a model of erythrocyte hemolysis in an in vitro experimenthttps://orcid.org/0000-0002-7450-3757РубниковичС. П.RubnikovichS. P.

Рубникович Сергей Петрович – член-корреспондент, д-р мед. наук, профессор, ректор

пр. Дзержинского, 83, 220083, г. Минск

Sergey P. Rubnikovich – Corresponding Member,Sc. (Med.), Professor, Rector

83, Dzerzhinsky Ave., 220083, Minsk

rubnikovichs@mail.ruhttps://orcid.org/0000-0002-1686-1820БекжановаО. Е.BekjanovaO. E.

Бекжанова Ольга Есеновна – д-р мед. наук, профес сор, заведующий кафедрой

ул. Фараби, 2, 100109, г. Ташкент

 

Olga Е. Bekjanova – D. Sc. (Med.), Professor, Head of the Department 

2, Farabi Str., 100109, Tashkent

bekjanovaolga@mail.ruhttps://orcid.org/0009-0007-7744-4469ХасановаЛ. Э.KhasanovaL. E.

Хасанова Лола Эмильевна – д-р мед. наук, профессор, заведующий кафедрой 

ул. Фараби, 2, 100109, г. Ташкент

Lola E. Khasanova – D. Sc. (Med.), Professor, Head of the Department 

2, Farabi Str., 100109, Tashkent

lola.e.khasanova@gmail.comhttps://orcid.org/0009-0004-5547-2037ШамсиеваШ. Ф.ShamsievaSh. F.

Шамсиева Шохиста Фазлиддиновна – ассистент 

ул. Фараби, 2, 100109, г. Ташкент

Shohista F. Shamsieva – Assistant 

2, Farabi Str., 100109, Tashkent

shamsiyevashohista8@gmail.comhttps://orcid.org/0009-0009-8516-172XАлимоваС. Х.AlimovaS. X.

Алимова Севара Хаитматовна – канд. мед. наук, доцент 

ул. Фараби, 2, 100109, г. Ташкент

Sevara X. Alimova – Ph. D. (Med.), Associate Professor 

2, Farabi Str., 100109, Tashkent

alimovasevara217@gmail.comhttps://orcid.org/0009-0000-9979-4821АстанакуловаМ. М.AstanakulovaM. M.

Астанакулова Муниса Мирзаевна – канд. мед. наук, доцент 

ул. Фараби, 2, 100109, г. Ташкент

Munisa M. Astanakulova – Ph. D. (Med.), Associate Professor 

2, Farabi Str., 100109, Tashkent

munisa.suyunova@mail.ruhttps://orcid.org/0009-0002-2890-0978БабаджановаН. Т.BabadjanovaN. T.

Бабаджанова Нодира Таировна – канд. мед. наук, ассистент 

ул. Фараби, 2, 100109, г. Ташкент

Nodira T. Babadjanova – Ph. D. (Med.), Assistant 

2, Farabi Str., 100109, Tashkent

Babadjanovanodira9@gmail.comhttps://orcid.org/0009-0002-3396-1441МирзаевХ. Ш.MirzaevX. Sh.

Мирзаев Хусан Шокиржонович – ассистент

ул. Фараби, 2, 100109, г. Ташкент

Xusan Sh. Mirzaev – Assistant 

2, Farabi Str., 100109, Tashkent

xusanmirzaev91@gmail.comБелорусский государственный медицинский университетBelarusian State Medical UniversityТашкентский государственный медицинский университетTashkent State Medical University20262705202623295103Copyright © Рубникович С.П., Бекжанова О.Е., Хасанова Л.Э., Шамсиева Ш.Ф., Алимова С.Х., Астанакулова М.М., Бабаджанова Н.Т., Мирзаев Х.Ш., 20262026Рубникович С.П., Бекжанова О.Е., Хасанова Л.Э., Шамсиева Ш.Ф., Алимова С.Х., Астанакулова М.М., Бабаджанова Н.Т., Мирзаев Х.Ш.Rubnikovich S.P., Bekjanova O.E., Khasanova L.E., Shamsieva S.F., Alimova S.X., Astanakulova M.M., Babadjanova N.T., Mirzaev X.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/1082

На сегодняшний день использование вместо известных ирригационных растворов новых антисептических препаратов привлекательно, поскольку последние имеют меньше побочных эффектов.

Цель исследования – оценить и сравнить цитотоксичность современных ирригационных растворов и антисептика Стоматидина в эксперименте in vitro.

Исследования проводились в четырех группах (n = 10): группа 1 – 3,0%-й р-р гипохлорита натрия (NaOCl); группа 2 – 2,0%-й р-р хлоргексидина (СНХ); группа 3 – антисептик Стоматидин; группа 4 – Стоматидин, активированный ультразвуком. 100,0 мкл каждого ирриганта добавляли к 2 мл разведенной суспензии эритроцитов, полученной от человека-добровольца.

Наличие у гемоглобина отчетливого спектра поглощения позволило оценивать степень гемолиза при помощи спектрофотометрии в единицах оптической плотности. С этой целью был проведен гемолитический анализ в микроцентрифужных пробирках, содержащих 10%-ю суспензию эритроцитов после термостатирования при температуре 37 °C в течение 60 мин. Процент гемолиза определялся с помощью спектрофотометрического считывания при 540 нм до и после термостатирования.

Увеличение процента гемолиза эритроцитов было максимальным у 3,0%-го р-ра NaOCl; далее – у 2,0%-го СНХ, минимальная цитотоксичность установлена у Стоматидина; увеличение процента гемолиза эритроцитов у Стоматидина и Стоматидина, активированного ультразвуком, не имело статистически значимых различий.

The use of new antiseptic preparations as irrigation solutions to replace well-known irrigants is an attractive option, as they have fewer side effects.

The purpose of the research was to evaluate and compare the cytotoxicity of modern irrigation solutions and the antiseptic Stomatidin in an in vitro experiment.

The study was conducted with four groups (n = 10): group 1 – 3.0 % sodium hypochlorite (NaOCl) solution; group 2 – 2.0 % chlorhexidine (CHX); group 3 – the antiseptic Stomatidin; and group 4 – ultrasound-activated Stomatidin. One hundred microliters of each irrigant were added to 2 mL of a diluted erythrocyte suspension obtained from a human volunteer.

The distinct absorption spectrum of hemoglobin enabled the assessment of the degree of hemolysis using spectrophotometry in units of optical density. For this purpose, a hemolytic analysis was performed in microcentrifuge tubes containing a 10 % erythrocyte suspension after incubation at 37 °C for 60 minutes. The percentage of hemolysis was determined by spectrophotometric reading at 540 nm before and after incubation.

The increase in the percentage of erythrocyte hemolysis was highest with the 3.0 % NaOCl solution, followed by 2.0 % CHX. The lowest cytotoxicity was observed with Stomatidin. The increase in the percentage of erythrocyte hemolysis for Stomatidin and ultrasound-activated Stomatidin showed no statistically significant differences.

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The authors declare that there are no conflicts of interest present.