vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2026-23-1-57-67vestim-1075Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEПротивоопухолевая активность in vitro глицирретовой кислоты и ее сочетаний с модуляторами кальциевой сигнализацииAntitumor activity in vitro of glycyrrhetinic acid and its combinations with calcium signaling modulatorsТерпинскаяТ. И.TerpinskayaT. I.

Терпинская Татьяна Ильинична – канд. биол. наук, доцент, вед. науч. сотрудник 

ул. Академическая, 28, 220072, г. Минск

Tatyana I. Terpinskaya – Ph. D. (Biol.), Associate Professor, Leading Researcher 

28, Akademicheskaya Str., 220072, Minsk

terpinskayat@mail.ruМаркевичК. В.MarkevichK. V.

Маркевич Карина Викторовна – мл. науч. сотрудник, аспирант

ул. Академическая, 28, 220072, г. Минск

Karina V. Markevich – Junior Researcher, Postgraduate Student 

28, Akademicheskaya Str., 220072, Minsk

karinamorozova_karina@mail.ruПолукошкоЕ. Ф.PolukoshkoE. F.

Полукошко Елена Федоровна – ст. науч. сотрудник

ул. Академическая, 28, 220072, г. Минск

Elena F. Polukoshko – Senior Researcher 

28, Akademicheskaya Str., 220072, Minsk

efpoluko@list.ruИнститут физиологии Национальной академии наук БеларусиInstitute of Physiology of the National Academy of Sciences of Belarus2026030320262315767Copyright © Терпинская Т.И., Маркевич К.В., Полукошко Е.Ф., 20262026Терпинская Т.И., Маркевич К.В., Полукошко Е.Ф.Terpinskaya T.I., Markevich K.V., Polukoshko E.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/1075

Исследована противоопухолевая активность in vitro глицирретовой кислоты и ее сочетаний с модуляторами кальциевого обмена клетки и ингибиторами кальций-связывающих белков.

Показано, что глицирретовая кислота дозозависимо подавляет рост клеток глиомы С6 и Hela. Усиление ингибирующего действия может быть достигнуто посредством сочетанного применения глицирретовой кислоты с А23187 (кальциевым ионофором), хлорпромазином (ингибитором кальмодулина), циклоспорином (ингибитором кальциневрина), U73122 (ингибитором фосфолипазы С), рутениевым красным (ингибитором ваниллоидных каналов временного рецепторного потенциала и митохондриального Са2+-унипортера), что обеспечивает более выраженный эффект, чем применение глицирретовой кислоты и указанных соединений по отдельности. Опухолевые клетки глиомы С6 и Hela были более чувствительны к глицирретовой кислоте, исследованным модуляторам кальциевого обмена клетки и сочетаниям глицирретовой кислоты с этими модуляторами, чем нормальные клетки первичной культуры головного мозга крысы.

The antitumor activity in vitro of glycyrrhetinic acid and its combinations with modulators of cellular calcium metabolism and inhibitors of calcium-binding proteins was studied. It was shown that glycyrrhetinic acid dose-dependently suppresses the growth of glioma C6 and HeLa. Enhancement of the inhibitory effect can be achieved by combining glycyrrhetinic acid with A23187 (calcium ionophore), chlorpromazine (calmodulin inhibitor), cyclosporine (calcineurin inhibitor), U73122 (phospholipase C inhibitor), ruthenium red (inhibitor of vanilloid channels of transient receptor potential and mitochondrial Ca2+-uniporter), which provides a more pronounced effect than the use of glycyrrhetinic acid and the above compounds separately. Tumor cells of glioma C6 and HeLa were more sensitive to glycyrrhetinnic acid, the studied modulators of cellular calcium metabolism and combinations of glycyrrhetinic acid with these modulators than normal cells of primary rat brain culture.

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The authors declare that there are no conflicts of interest present.